Adhesion ofPseudomonas aeruginosa to collagen biomaterials: Effect of amikacin and ciprofloxacin on the colonization and survival of the adherent organisms

Abstract

The adherence of P. aeruginosa to collagen membrane, sponge, and to a new anti-infective COLL dressing and the susceptibility of the organisms attached to the biomaterials to amikacin were investigated in vitro. After 17 h of attachment, the bacteria demonstrated an increased resistance to amikacin compared with their free-floating counterparts. Amikacin, even at a concentration exceeding 150 times the minimal bactericidal concentration (MBC) for the strain tested, did not eradicate the attached bacteria from the surface of collagen membrane. However, when the drug at a high concentration (over 16 times the minimal inhibitory concentration, MIC) was present in the incubation medium before it had been inoculated with P. aeruginosa, a reduction of 2 log10 units in the organisms adherent to the surface of collagen membrane was observed. We conclude that slow release of the antibiotic from the COLL dressing could control the bacterial colonization on the surface. In fact, the released amikacin at the final concentration of 32 times the MBC reduced the number of adherent bacteria by 6 log10 units. In contrast, ciprofloxacin at the same final bactericidal concentration completely eradicated the bacteria from the surface of COLL dressing. However, as ciprofloxacin is not recommended for use as a topical antimicrobial agent, a further search is needed to find an agent with a similar anticolonization activity.

Improved collagen bilayer dressing for the controlled release of drugs

“…The SEM observation shows that the pore size of the sponge varies from 500 to 800 μm, because of the swelling nature of the material in aqueous medium, which allowed the drug to enter into the sponges and to bind on drying. Another superiority of the bilayer system over the other system is evident from the water‐vapor transmission study, a value of 1570 ± 25 g/m 2 /day1, which is more than the water‐vapor transmission rate of the commercially available dressings currently employed, for example, Comfeel (Coloplast A/s), Dermiflex (Johnson & Johnson), Duroderm (Conva Tec Ltd.), Metoderm (Conva Tec Ltd.), where the water‐vapor transmission rate ranges from 823 to 308 g/m 2 /day 29. Water‐uptake studies in the dressing show a high swelling rate at physiological pH, which confirms the high efficiency of dressing to absorb wound exudates.…”

Possible role of the adhesin ace and collagen adherence in conveying resistance to disinfectants on Enterococcus faecalis

“…This study found that E. faecalis that adhered to collagen was more resistant against IKI and CHX at different periods. One study that has investigated the effect of adhesion by Pseudomonas aeruginosa to collagen membrane also found significant resistance of the adhering bacteria against an aminoglycoside (22). However, the authors believed that this effect was the result of biofilm formation on the collagen membrane surface, a well‐recognized mechanism of antimicrobial resistance.…”

Preparation and drug release activity of scaffolds containing collagen and poly(caprolactone)

“…In the recent years, a number of polymeric materials have been studied as promising drug delivery system to provide improved delivery efficiency and to reduce side effects of drug toxicity 1. Collagen based biomaterials are considered as most promising substances for wound healing and skin regeneration 2. Successful wound graft out come requires adherence to wounds, histocompatibility, control of fluids loss and infection, absence of antigenecity and toxicity, mechanical stability and compliance, cost effectiveness, and ease of availability 3, 4.…”

“…Several antibiotics have been investigated as agents for the reduction of wound contaminations and to improve wound healing. But, amikacin and gentamycin have been implanted into the scaffolds without loss of its activity 2, 14. Moreover, these antibiotics are active against both gram‐positive and gram‐negative bacteria.…”