Adhesion, Proliferation and Migration of NIH/3T3 Cells on Modified Polyaniline Surfaces

Abstract

Polyaniline shows great potential and promises wide application in the biomedical field thanks to its intrinsic conductivity and material properties, which closely resemble natural tissues. Surface properties are crucial, as these predetermine any interaction with biological fluids, proteins and cells. An advantage of polyaniline is the simple modification of its surface, e.g., by using various dopant acids. An investigation was made into the adhesion, proliferation and migration of mouse embryonic fibroblasts on pristine polyaniline films and films doped with sulfamic and phosphotungstic acids. In addition, polyaniline films supplemented with poly (2-acrylamido-2-methyl-1-propanesulfonic) acid at various ratios were tested. Results showed that the NIH/3T3 cell line was able to adhere, proliferate and migrate on the pristine polyaniline films as well as those films doped with sulfamic and phosphotungstic acids; thus, utilization of said forms in biomedicine appears promising. Nevertheless, incorporating poly (2-acrylamido-2-methyl-1-propanesulfonic) acid altered the surface properties of the polyaniline films and significantly affected cell behavior. In order to reveal the crucial factor influencing the surface/cell interaction, cell behavior is discussed in the context of the surface energy of individual samples. It was clearly demonstrated that the lesser the difference between the surface energy of the sample and cell, the more cyto-compatible the surface is.

Intact polyaniline coating as a conductive guidance is beneficial to repairing sciatic nerve injury

“…Immersion of PANi‐coated conduits into saline for at least 5 days can remove the doping acid and other small molecules introduced in the process of synthesis and thus reduce the possible cytotoxicity . The cytocompatibility of the intact PANi coating on zein film showed good for both PC 12 cells and NIH 3 T3 cells (Figure ), which was consistent with published works …”

“…[33][34][35] The cytocompatibility of the intact PANi coating on zein film showed good for both PC 12 cells and NIH 3 T3 cells (Figure 2), which was consistent with published works. 36,37 Direct nerve repair is preferred when the gap is small and two ends can be approximated with minimal tension. 38 When the gap is longer than 5 mm, direct microsurgery will cause the tension of nerve fibers.…”

Characterization of a nontoxic pyomelanin pigment produced by the yeast Yarrowia lipolytica

“…The first one is used in standard procedures for testing nanomaterial and for studying material biocompatibility. 45 SDs were less than 14%. DHHP, 1,1-diphenyl-2-picrylhydrazyl ingredients.…”

“…This was performed through three different but complementary methods for two different cell lines, namely mouse fibroblast (NIH3T3) and human keratinocyte (HaCaT). The first one is used in standard procedures for testing nanomaterial and for studying material biocompatibility . Keratinocytes were selected since it is the most abundant cells of epithelial layer of the skin.…”

Dual Crosslinked Collagen/Chitosan Film for Potential Biomedical Applications

“…The cell compatibility was detected according to previously described procedures [37]. The test samples (films Col/Chi, Col/Chi-Ta and DC-Col/Chi) were sterilized by UV radiation prior to testing.…”