Adhesion to porcine squamous epithelium of saccharide and protein moieties of Lactobacillus fermentum strain 104-S

Henriksson, A.; Conway, Patricia L.

doi:10.1099/00221287-138-12-2657

Cited by 23 publications

(10 citation statements)

References 13 publications

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“…Several studies demonstrated the presence of a layer of gram-positive rod-shaped bacteria on different epithelial cells:gastric nonsecretory keratinized epithelium of pigs, mice and rats (1,(8)(9)(10), porcine oesophageal samples (2) and ileal porcine mucus (11).…”

Section: Introductionmentioning

confidence: 99%

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Hautefort¹

2000

Microbial Ecology in Health and Disease

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Section: Introductionmentioning

confidence: 99%

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Hautefort¹

2000

Microbial Ecology in Health and Disease

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“…to non-secreting tissue [21], suggesting that the There was no clear trend as to which area was the smooth colony forming variants are equipped with most specific in its composition for the three piglets;…”

Section: Discussionmentioning

confidence: 96%

Distribution of lactobacilli in the porcine gastrointestinal tract

Henriksson

André

Conway

1995

FEMS Microbiology Ecology

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Characteristics of the lactobacilli colonizing the various regions of the porcine gastrointestinal tract were investigated. The lumenal contents from the gastric, jejunal, cecal and colonic regions, and biopsies from the gastric non‐secreting and secreting regions were homogenized and serial dilutions were spread on Rogosa agar and colonies (50 per region and animal) were randomly sampled. The lactobacillus isolates were grouped according to their protein profiles of lysozyme‐treated whole cells. Several different groups of lactobacillus could be detected. Specific groups were associated and often unique for the stomach, jejunal, cecal and colonic regions of the gastrointestinal tract. A few groups were uniformly distributed through the tract. Although published studies of lactobacilli colonizing stomach mucosa have been restricted mainly to the non‐secreting region, significant levels were also detected on the secreting stomach tissue. Population densities ranged from 2 × 104 to 2 × 106 CFU per cm2 on both the secretory and non‐secretory regions. Of the isolates which colonized the gastric secreting epithelium, 26% formed smooth colonies and 74% formed rough colonies. In contrast, the non‐secreting epithelium was mainly colonized by lactobacilli forming smooth colonies (67%). From this study, one can suggest that different lactobacilli are associated with specific regions of the gastrointestinal tract, and that some specific groups appear to be restricted to defined regions.

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“…This confirms that cell surface components play a major role in the specific binding to Peyer's patches and that as protease reduced the binding capacity of L. fermentum 003, it is most probably proteinaceous components that are involved in binding to Peyer's patches. In most cases, bacterial cell surface components have been shown to contribute to the adhesive capacity of lactobacilli (Henriksson & Conway, 1992;Greene et al, 1994;Kapczynski et al, 2000;Tuomola et al, 2000;Lorca et al, 2002). It was suggested that the binding capacity of L. acidophilus strains adherence to collagen and fibronectin was lost after treatment with proteases (Lorca et al, 2002).…”

Section: Effect Of Enzymatic and Chemical Pretreatment Of L Fermentumentioning

confidence: 99%

Characteristics of the adhesion of PCC^Â®Lactobacillus fermentumVRI 003 to Peyer's patches

Kang

Conway

2006

FEMS Microbiology Letters

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The characteristics of the adhesion of PCC Lactobacillus fermentum VRI 003 to Peyer's patches was studied in vitro. The adhesion of L. fermentum 003 was strongly inhibited in the presence of d-mannose and methyl-alpha-d-mannoside although other carbohydrates tested, such as N-acetyl-glucosamine, d-galactose, d-glucose and l-fucose, did not affect the adhesion. Lactobacillus fermentum 003 was shown to strongly attach to mannose immobilized on a surface using BSA, suggesting that L. fermentum 003 specifically adhered to mannose-containing molecule(s). Pretreatment of L. fermentum 003 with proteinase K and trypsin decreased the adhesive capacity and bacterial surface extracts diminished adhesion of L. fermentum 003 indicating that cell surface proteins are involved in adhesion to Peyer's patches. It was concluded that a mannose-specific protein mediated adhesion of L. fermentum 003 to the Peyer's patches.

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Adhesion to porcine squamous epithelium of saccharide and protein moieties of Lactobacillus fermentum strain 104-S

Cited by 23 publications

References 13 publications

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Distribution of lactobacilli in the porcine gastrointestinal tract

Characteristics of the adhesion of PCC^Â®Lactobacillus fermentumVRI 003 to Peyer's patches

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Adhesion to porcine squamous epithelium of saccharide and protein moieties of Lactobacillus fermentum strain 104-S

Cited by 23 publications

References 13 publications

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Adhesion to the digestive mucosa is not sufficient for durable persistence of differentLactobacillus fermentumstrains in the digestive tract of mice

Distribution of lactobacilli in the porcine gastrointestinal tract

Characteristics of the adhesion of PCCÂ®Lactobacillus fermentumVRI 003 to Peyer's patches

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Characteristics of the adhesion of PCC^Â®Lactobacillus fermentumVRI 003 to Peyer's patches