Adhesive substrate-modulation of adaptive immune responses

Absence of Immune Responses with Xenogeneic Collagen and Elastin

“…The same might apply to studies with ECMp in a murine system, where bovine collagen I and vitronectin influenced the maturation of murine blood monocytederived iDC and caused higher IL-12p40 release, T cell activation, and proliferation. 44 However, it has also been reported, that murine bone marrow-derived DC were completely unresponsive to maturation stimuli after initial culture on collagen I, 25 which is consistent with our results. Whether the lack of iDC maturation demonstrated for all ECMp tested in our study will also result in reduced T cell proliferation within a mixed lymphocyte culture is not clear.…”

Material-driven fibronectin fibrillogenesis to engineer cell function.

“…Understanding fundamental mechanisms of leukocyte and biomaterial interactions are important in this regard. It has been shown that adsorbed proteins on implanted biomaterials modulate inflammatory responses (Acharya et al, 2008). As said before (see 'Matrix protein interaction with synthetics surfaces') after implantation of the biomaterial, some ECM proteins spontaneously adsorb onto synthetic biomaterials (Anderson, et al, 2008;Chang et al, 2009) and can modulate leukocyte adhesion and inflammatory responses to implants (Anderson et al, 2008;Hunter et al, 1999;Jenney & Anderson, 2000;Keselowsky et al, 2007;Shen & Horbett, 2001;Tang & Eaton, 1993).…”

“…As said before (see 'Matrix protein interaction with synthetics surfaces') after implantation of the biomaterial, some ECM proteins spontaneously adsorb onto synthetic biomaterials (Anderson, et al, 2008;Chang et al, 2009) and can modulate leukocyte adhesion and inflammatory responses to implants (Anderson et al, 2008;Hunter et al, 1999;Jenney & Anderson, 2000;Keselowsky et al, 2007;Shen & Horbett, 2001;Tang & Eaton, 1993). The adaptive immune response to the new biomaterial can be altered by these inflammatory responses (Acharya et al, 2009;Acharya et al, 2008).…”

“…The RGD integrin binding motif is present in several ECM proteins including FN and VN (Dickinson et al, 1994;Humphries et al, 2006), and is one of the motives that has been investigated with numerous cell types (Fittkau et al, 2005;Mann et al, 2001;Schaffner & Dard, 2003). However, the role that the specific DCs integrin binding plays in the modulation of DCs functions has only recently been explored (Acharya et al, 2008;Acharya et al, 2010). DCs adhesion to ECM proteins is of interest physiologically as DCs act as sentinels constantly patrolling the body and they reside for much of their lifetime in connective tissues comprised largely of ECM proteins in both lymphoid and non-lymphoid organs (Liu & Nussenzweig, 2010;Schmidt et al, 2012).…”

“…It has been shown that DCs interactions with various biomaterials modulate DCs functions (Babensee, 2008;Jones, 2008) and that DCs express multiple integrins (Ammon et al, 2000;Brown et al, 1997;Dalgaard et al, 2005;Dubey et al, 1995;Hamakawa et al, 2006;Lu et al, 1995;Sung et al, 2006), but there are surprisingly few investigations into the effects of integrin binding to ECM proteins on DCs maturation and only a few investigations have begun to elucidate the mechanisms involved into the modulation of DC functions (Acharya et al, 2009;Acharya et al, 2008;Acharya et al, 2011;Brand et al, 1998;Kohl et al, 2007;Shankar et al, 2010). DCs have been cultured on surfaces presenting a uniform gradient of the integrin targeting RGD peptide and demonstrate the utility of the surface density gradient platform as a high-throughput method to investigate RGD densitydependent DCs adhesive responses (Acharya et al, 2010).…”