Adipocytes Are the Control Tower That Manages Adipose Tissue Immunity by Regulating Lipid Metabolism

Park, Jeu; Sohn, Jin Ho; Han, Sun-Hee; Park, Yoon Jeong; Choe, Sung Sik; Kim, Jae Bum

doi:10.3389/fimmu.2020.598566

Cited by 9 publications

(8 citation statements)

References 110 publications

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“…These DEGs included more than 20 genes related to antigen processing and presentation, innate immunity and chemokines, which connected immune and inflammatory response with lipid metabolism during adipocyte hypertrophy [ 23 ], and were indispensable for the cross-talk between adipocytes and immune cells in adipose tissues [ 63 ]. Apparently, HG-induced adipocytes exhibited a pronounced “immune-like” capability that could activate and recruit immune cells to dialogue with themselves [ 64 ]. This could be related to the positive role of the immune-inflammatory response in maintaining the adipose tissue microenvironment to promote healthy adipocyte expansion [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Jiang,

Zhang,

Miao

et al. 2024

CIMB

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Glucose is a major energy substrate for porcine adipocytes and also serves as a regulatory signal for adipogenesis and lipid metabolism. In this study, we combined transcriptome and metabolome analyses to reveal the underlying regulatory mechanisms of high glucose (HG) on adipogenesis by comparing differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) identified in porcine adipocytes. Results showed that HG (20 mmol/L) significantly increased fat accumulation in porcine adipocytes compared to low glucose (LG, 5 mmol/L). A total of 843 DEGs and 365 DAMs were identified. Functional enrichment analyses of DEGs found that multiple pathways were related to adipogenesis, lipid metabolism, and immune-inflammatory responses. PPARγ, C/EBPα, ChREBP, and FOS were identified as the key hub genes through module 3 analysis, and PPARγ acted as a central regulator by linking genes involved in lipid metabolism and immune-inflammatory responses. Gene-metabolite networks found that PPARγ-13-HODE was the most important interaction relationship. These results revealed that PPARγ could mediate the cross-talk between adipogenesis and the immune-inflammatory response during adipocyte maturation. This work provides a comprehensive view of the regulatory mechanisms of glucose on adipogenesis in porcine adipocytes.

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Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Jiang,

Zhang,

Miao

et al. 2024

CIMB

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“…We found that licoricidin administration to mice by both oral gavage and fat pad injection effectively stimulated beige fat thermogenesis but had little effect on BAT thermogenesis. A possible explanation for this result is that adipose tissue is heterogeneous, and its function is dynamically orchestrated by the microenvironment within the tissue such as T cells and macrophages 43 , 44 . Alternatively, given that both β3-AR and UCP1 are highly enriched in BAT 45 , 46 , low doses of licoricidin may be insufficient to further increase BAT thermogenesis, demonstrating a beige fat-specific function of licoricidin in thermogenesis.…”

Section: Discussionmentioning

confidence: 99%

Vertical sleeve gastrectomy-derived gut metabolite licoricidin activates beige fat thermogenesis to combat obesity

Jin¹,

Meng²,

Xiao³

et al. 2023

Theranostics

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Obesity is a chronic metabolic disease, affecting individuals throughout the world. Bariatric surgery such as vertical sleeve gastrectomy (VSG) provides sustained weight loss and improves glucose homeostasis in obese mice and humans. However, the precise underlying mechanisms remain elusive. In this study, we investigated the potential roles and the mechanisms of action of gut metabolites in VSG-induced anti-obesity effect and metabolic improvement. Methods: High-fat diet (HFD)-fed C57BL/6J mice were subjected to VSG. Energy dissipation in mice was monitored using metabolic cage experiments. The effects of VSG on gut microbiota and metabolites were determined by 16S rRNA sequencing and metabolomics, respectively. The metabolic beneficial effects of the identified gut metabolites were examined in mice by both oral administration and fat pad injection of the metabolites. Results: VSG in mice greatly increased thermogenic gene expression in beige fat, which was correlated with increased energy expenditure. VSG reshaped gut microbiota composition, resulting in elevated levels of gut metabolites including licoricidin. Licoricidin treatment promoted thermogenic gene expression in beige fat by activating the Adrb3-cAMP-PKA signaling pathway, leading to reduced body weight gain in HFD-fed mice. Conclusions: We identify licoricidin, which mediates the crosstalk between gut and adipose tissue in mice, as a VSG-provoked anti-obesity metabolite. Identification of anti-obesity small molecules should provide new insights into treatment options for obesity and its associated metabolic diseases.

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“…Pref‐1 inhibits the adipogenic differentiation of adipocytes by inhibiting the early adipogenic differentiation factors C/EBPβ and C/EBPδ (da Silva & Durandt, 2020; Park & Kim, 2020; Unger, 2003). Park et al (2020) proved that with the increase of adipogenic differentiation time, the expression level of CAVIN3 gradually increased, and the expression of Pref‐1 gradually decreased in 3T3‐L1 cells. Further studies have found that CAVIN3 promotes adipogenic differentiation by down‐regulating the tumor necrosis factor α converting enzyme (TACE) and inhibiting the function of Pref‐1 (Park & Kim, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Adipose tissue is an important energy storage organ, which is widely involved in the biological metabolic processes of the body. Fat deposition affects the physiological functions and economic traits of animals (Park et al, 2020). Aberrant fat deposition may lead to poor disease resistance, decrease the production performance of pigs, and reduce feeding efficiency.…”

Section: Introductionmentioning

confidence: 99%

Caveolae‐associated protein 3 promotes adipogenic differentiation of porcine preadipocytes by promoting extracellular signal‐regulated kinase phosphorylation

Shi

Yang

Huang

et al. 2023

Animal Science Journal

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Fat deposition is one of the key factors affecting the economic development of pig husbandry. The aim of this study was to investigate the expression characteristics of caveolae‐associated protein 3 (CAVIN3) and to elucidate its effect and mechanism on adipogenic differentiation of porcine preadipocytes. Cell transfection, quantitative reverse transcription polymerase chain reaction (qRT‐PCR), western blot, and oil red O staining were used to detect the effect of CAVIN3 on the differentiation of porcine preadipocytes. The results showed that CAVIN3 was expressed in various tissues, with higher expression in adipose tissue, differentially expressed during cell adipogenic differentiation, and mainly distributed in the cytoplasm. Functional studies showed that, after CAVIN3 interference in preadipocytes, the expression of adipogenic factors and the content of lipid droplets were significantly decreased (p < 0.05). The results were reversed after CAVIN3 was overexpressed. The mechanism research showed that LY3214996 inhibited the extracellular signal‐regulated kinase (ERK) phosphorylation and further inhibited lipogenic factors expression. Overexpression of CAVIN3 attenuates the inhibitory effect of LY3214996 on ERK phosphorylation and attenuates its inhibitory effect on adipogenic differentiation. Therefore, this study demonstrated that CAVIN3 promotes the differentiation of porcine preadipocytes by promoting ERK phosphorylation. The present study can lay a theoretical foundation for further studying the molecular mechanism of porcine fat deposition.

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Adipocytes Are the Control Tower That Manages Adipose Tissue Immunity by Regulating Lipid Metabolism

Cited by 9 publications

References 110 publications

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes

Vertical sleeve gastrectomy-derived gut metabolite licoricidin activates beige fat thermogenesis to combat obesity

Caveolae‐associated protein 3 promotes adipogenic differentiation of porcine preadipocytes by promoting extracellular signal‐regulated kinase phosphorylation

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