Adipocytokine, omentin inhibits doxorubicin-induced H9c2 cardiomyoblasts apoptosis through the inhibition of mitochondrial reactive oxygen species

Kazama, Kyosuke; Okada, Muneyoshi; Yamawaki, Hideyuki

doi:10.1016/j.bbrc.2015.01.032

Cited by 39 publications

(27 citation statements)

References 40 publications

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“…Omentin suppresses agonist-stimulated hypertrophic response of cultured cardiac myocytes via activation of AMPK. Omentin is also reported to prevent doxorubicin-inducible cardiomyocyte death in vitro via suppression of mitochondrial reactive oxygen species 83) . Thus, these observations suggest that omentin serves as a cardioprotective adipocytokine (Fig.…”

Section: Resultsmentioning

confidence: 99%

Adipocytokines in Cardiovascular and Metabolic Diseases

Ouchi

2016

JAT

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Obesity, particularly excess visceral fat accumulation, is highly associated with the development of metabolic syndrome and atherosclerotic cardiovascular disease. Adipose tissue produces a variety of secreted proteins, referred to as adipocytokines, which directly affect nearby or remote organs. Dysregulation of adipocytokines caused by obese conditions contributes to the pathogenesis of various metabolic and cardiovascular disorders. This review focuses on the significance of several adipocytokines that potentially exert beneficial actions on obesity-related diseases, including atherosclerosis and ischemic heart disease.J Atheroscler Thromb, 2016; 23: 645-654.

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Section: Resultsmentioning

confidence: 99%

Adipocytokines in Cardiovascular and Metabolic Diseases

Ouchi

2016

JAT

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“…High levels of ROS are associated with metabolic diseases like diabetes [16,20,36], which may contribute to the loss of myoblast function, increase myoblast cell death [33] and further exacerbate muscle repair in aging. Fulle and colleagues [25] have demonstrated that a high percentage of the myogenic precursor cells from elderly muscles undergo apoptosis triggered by mitochondrial-associated caspase-9 and this appears to be closely linked to the high ROS levels that are found in aged muscles [20].…”

Section: Introductionmentioning

confidence: 99%

Dietary resveratrol confers apoptotic resistance to oxidative stress in myoblasts

Haramizu

Asano

Butler

et al. 2017

The Journal of Nutritional Biochemistry

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High levels of reactive oxygen species (ROS) contributes to muscle cell death in aging and disuse. We have previously found that resveratrol can reduce oxidative stress in response to aging and hindlimb unloading in rodents in vivo, but it was not known if resveratrol would protect muscle stem cells during repair or regeneration when oxidative stress is high. To test the protective role of resveratrol on muscle stem cells directly, we treated the C2C12 mouse myoblast cell line with moderate (100 μM) or very high (1 mM) levels of H2O2; in the presence or absence of resveratrol. The p21 promoter activity declined in myoblasts in response to high ROS and this was accompanied a greater nuclear to cytoplasmic translocation of p21 in a dose dependent matter in myoblasts as compared to myotubes. Apoptosis, as indicated by TdT-mediated dUTP nick-end (TUNEL) labeling was greater in C2C12 myoblasts as compared to myotubes (P< 0.05) after treatment with H2O2. Caspase-9, -8, and -3 activities were elevated significantly (P< 0.05) in myoblasts treated with H2O2. Myoblasts were more susceptible to ROS-induced oxidative stress than myotubes. We treated C2C12 myoblasts with 50 μM of resveratrol for periods up to 48 h to determine if myoblasts could be rescued from high ROS-induced apoptosis by resveratrol. Resveratrol reduced the apoptotic index, and significantly reduced the ROS-induced caspase-9, -8, and -3 activity in myoblasts. Furthermore, Bcl-2 and the Bax/Bcl-2 ratio were partially rescued in myoblasts by resveratrol treatment. Similarly, muscle stem cells isolated from mouse skeletal muscles showed reduced Sirt1 protein abundance with H2O2 treatment but this could be reversed by resveratrol. Reduced apoptotic susceptibility in myoblasts as compared to myotubes to ROS is regulated at least in part, by enhanced p21 promoter activity and nuclear p21 location in myotubes. Resveratrol confers further protection against ROS by improving Sirt1 levels and increasing antioxidant production, which reduces mitochondrial associated apoptotic signaling, and cell death in myoblasts.

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“…Decreased serum level of omentin-1 was reported in polycystic ovary syndrome [6] , diabetes [7] and coronary artery disease [8] . Moreover, omentin was reported to prevent apoptosis of cardiomyoblasts via inhibition of mitochondrial ROS production [9] .…”

Section: Introductionmentioning

confidence: 99%

Omentin-1 in serum and seminal plasma correlate with semen quality

et al. 2017

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Introduction: Adipokines are differently regulated in the male reproductive tract and they were reported to affect sperm functionality. Serum omentin-1 was reported to have a range of effects on various cell types. Aim: To explore the presence of omentin-1in human semen, to investigate its relation with semen parameters in both fertile and infertile men and to compare its serum and seminal plasma levels in particular conditions such as; smoking, varicocele and leukocytospermia. Methods: Thirty nine infertile and 26 fertile men were enrolled in this case control study. They were subjected to history taking, clinical/physical examination, semen analysis, and measurement of omentin-1 in serum and seminal plasma, DNA fragmentation index (DFI) and seminal reactive oxygen species (ROS). Results: Omentin-1 was detected in all analyzed semen and serum samples (n=65). The concentration of omentin-1 was significantly higher in seminal plasma than in serum. Serum and seminal omentin-1 were positively correlated with sperm concentration and percentage of progressive motility and were negatively correlated with percentage of abnormal forms, DFI and ROS. The mean seminal plasma level of omentin-1 was significantly decreased in smokers and in patients with leukocytospermia. The mean serum level of omentin-1 was significantly decreased in patients with varicocele. Both serum and seminal omentin-1 levels were significantly lower in infertile patients than fertile men. Conclusions: Semen and serum Omentin-1 correlate with semen quality. In cases of leukocytospermia and smoking, seminal omentin-1 concentrations were decreased, suggesting that it may play a regulatory role in inflammation of the male reproductive system.

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Adipocytokine, omentin inhibits doxorubicin-induced H9c2 cardiomyoblasts apoptosis through the inhibition of mitochondrial reactive oxygen species

Cited by 39 publications

References 40 publications

Adipocytokines in Cardiovascular and Metabolic Diseases

Adipocytokines in Cardiovascular and Metabolic Diseases

Dietary resveratrol confers apoptotic resistance to oxidative stress in myoblasts

Omentin-1 in serum and seminal plasma correlate with semen quality

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