Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds

Kang, Xihai; Xie, Yizhen; Powell, Heather M.; Lee, L. James; Belury, Martha A.; Lannutti, John J.; Kniss, Douglas A.

doi:10.1016/j.biomaterials.2006.08.052

Cited by 122 publications

(90 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCL and PU differ in mechanical properties, degradation profile and possibly in cellular interaction and in vivo behavior. PCL has been used with murine embryonic precursor cells to engineer fat tissue (Kang et al 2007) but to the best of our knowledge, this is the first study that evaluates the potential of PCL scaffolds with human adipose tissuederived precursor cells for adipose tissue engineering. PCL is a relatively stiff and slowly degradable polymer that can be easily processed into scaffolds, has been tested intensively and is approved in the form of Osteomesh by the FDA for tissue engineering (Woodruff and Hutmacher 2010).…”

Section: Assessment Of In Vivo Adipogenesismentioning

confidence: 98%

Engineering of vascularized adipose constructs

et al. 2011

View full text Add to dashboard Cite

Adipose tissue engineering offers a promising alternative to the current surgical techniques for the treatment of soft tissue defects. It is a challenge to find the appropriate scaffold that not only represents a suitable environment for cells but also allows fabrication of customized tissue constructs, particularly in breast surgery. We investigated two different scaffolds for their potential use in adipose tissue regeneration. Sponge-like polyurethane scaffolds were prepared by mold casting with methylal as foaming agent, whereas polycaprolactone scaffolds with highly regular stacked-fiber architecture were fabricated with fused deposition modeling. Both scaffold types were seeded with human adipose tissue-derived precursor cells, cultured and implanted in nude mice using a femoral arteriovenous flow-through vessel loop for angiogenesis. In vitro, cells attached to both scaffolds and differentiated into adipocytes. In vivo, angiogenesis and adipose tissue formation were observed throughout both constructs after 2 and 4 weeks, with angiogenesis being comparable in seeded and unseeded constructs. Fibrous tissue formation and adipogenesis were more pronounced on polyurethane foam scaffolds than on polycaprolactone prototyped scaffolds. In conclusion, both scaffold designs can be effectively used for adipose tissue engineering.

show abstract

Section: Assessment Of In Vivo Adipogenesismentioning

confidence: 98%

Engineering of vascularized adipose constructs

et al. 2011

View full text Add to dashboard Cite

show abstract

“…In addition to the above-mentioned tissues, the feasibilities of using nanofiber scaffolds to culture other stem cells [155][156][157][158][159][160] , and tissues such as heart [161,162] and ligament [163,164] have been reported.…”

Section: Othersmentioning

confidence: 98%

Applications of electrospun nanofibers

et al. 2008

View full text Add to dashboard Cite

“…However, PCL degrades at a significantly slower rate than PLA, PGA and PLGA [256]. This slow degradation makes PCL less attractive for this type of biomedical application, but more attractive for sutures, longterm implants and controlled-release applications [250].…”

Section: Polyurethanes (Pus)mentioning

confidence: 99%

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

et al. 2013

View full text Add to dashboard Cite

a b s t r a c tDiabetic foot ulcers (DFUs) are a chronic, non-healing complication of diabetes that lead to high hospital costs and, in extreme cases, to amputation. Diabetic neuropathy, peripheral vascular disease, abnormal cellular and cytokine/chemokine activity are among the main factors that hinder diabetic wound repair. DFUs represent a current and important challenge in the development of novel and efficient wound dressings. In general, an ideal wound dressing should provide a moist wound environment, offer protection from secondary infections, remove wound exudate and promote tissue regeneration. However, no existing dressing fulfills all the requirements associated with DFU treatment and the choice of the correct dressing depends on the wound type and stage, injury extension, patient condition and the tissues involved. Currently, there are different types of commercially available wound dressings that can be used for DFU treatment which differ on their application modes, materials, shape and on the methods employed for production. Dressing materials can include natural, modified and synthetic polymers, as well as their mixtures or combinations, processed in the form of films, foams, hydrocolloids and hydrogels. Moreover, wound dressings may be employed as medicated systems, through the delivery of healing enhancers and therapeutic substances (drugs, growth factors, peptides, stem cells and/or other bioactive substances). This work reviews the state of the art and the most recent advances in the development of wound dressings for DFU treatment. Special emphasis is given to systems employing new polymeric biomaterials, and to the latest and innovative therapeutic strategies and delivery approaches.

show abstract

Adipogenesis of murine embryonic stem cells in a three-dimensional culture system using electrospun polymer scaffolds

Cited by 122 publications

References 54 publications

Engineering of vascularized adipose constructs

Engineering of vascularized adipose constructs

Applications of electrospun nanofibers

Recent advances on the development of wound dressings for diabetic foot ulcer treatment—A review

Contact Info

Product

Resources

About