Adipogenic miRNA and meta-signature miRNAs involved in human adipocyte differentiation and obesity

Shi, Chunmei; Huang, Fei; Gu, Xue‐Ping; Zhang, Min; Wen, Juan; Wang, Xing; You, Lianghui; Cui, Xianwei; Ji, Chenbo; Guo, Xirong

doi:10.18632/oncotarget.8518

Cited by 99 publications

(79 citation statements)

References 34 publications

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“…More complete understanding of fat deposition mechanism is vital for beef cattle breeding and beef production. MicroRNAs are important post-transcriptional molecules regulating adipogenesis and lipid accumulation as previously discovered35. However, their precise roles of miRNAs in bovine fat deposition are far from clear.…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Guo

Zhang

Huang

et al. 2017

Sci Rep

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MicroRNAs (miRNAs) are important post-transcriptional regulators involved in animal adipogenesis, however, their roles in bovine fat deposition remain poorly understood. In the present study, we conducted a comparative RNA sequencing to identify the key miRNAs involved in beef lipid accumulation by comparing the backfat small RNA samples between Wagyu (high intramuscular fat) and Holstein (moderate intramuscular fat) cattle. Fifteen miRNAs such as bta-miR-142-3p, bta-miR-379, bta-miR-196a, bta-miR-196b, bta-miR-30f and bta-miR-2887 were identified to have a higher expression level in Wagyu cattle compared with Holstein, whereas bta-miR-320a, bta-miR-874 and bta-miR-1247-3p had a lower expression level in Wagyu. Furthermore, a total of 1345 potential target genes of differentially expressed miRNAs were predicted using bioinformatics tools, in which PPARα and RXRα were known to play a critical role in adipocyte differentiation and lipid metabolism. In conclusion, the present study constructed a high-throughput RNA sequencing screen and successfully identified miRNAs such as bta-miR-874, bta-miR-320a and bta-miR-196b which may affect beef fat deposition. The present findings may provide a theoretical foundation for the utilization of beef cattle germplasm resources.

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Section: Discussionmentioning

confidence: 99%

Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Guo

Zhang

Huang

et al. 2017

Sci Rep

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“…The dysfunction of miRNAs has been associated with a large number of diseases. For instance, the importance of miR-21 in different types of diabetes mellitus has been described by Sekar et al [8], and the miRNAs of the hsa-let-7 family and others are associated with obesity and related metabolic diseases [9]. miRNAs also participate in arthritic diseases [10], kidney disease [11], cardiovascular diseases [12], etc.…”

Section: Introductionmentioning

confidence: 99%

Tools for Sequence-Based miRNA Target Prediction: What to Choose?

Riffo‐Campos

Riquelme

Brebi-Mieville

2016

IJMS

354

232

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MicroRNAs (miRNAs) are defined as small non-coding RNAs ~22 nt in length. They regulate gene expression at a post-transcriptional level through complementary base pairing with the target mRNA, leading to mRNA degradation and therefore blocking translation. In the last decade, the dysfunction of miRNAs has been related to the development and progression of many diseases. Currently, researchers need a method to identify precisely the miRNA targets, prior to applying experimental approaches that allow a better functional characterization of miRNAs in biological processes and can thus predict their effects. Computational prediction tools provide a rapid method to identify putative miRNA targets. However, since a large number of tools for the prediction of miRNA:mRNA interactions have been developed, all with different algorithms, the biological researcher sometimes does not know which is the best choice for his study and many times does not understand the bioinformatic basis of these tools. This review describes the biological fundamentals of these prediction tools, characterizes the main sequence-based algorithms, and offers some insights into their uses by biologists.

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“…MiR-346 regulates hBMSCs osteoblast differentiation by targeting GSK-3β and activating the Wnt/β-catenin pathway [31]. Moreover, miR-27b has been verified to participate in lipid metabolism and adipogenesis [32,33], which is important for revealing these mechanisms and finding treatments for obesity, diabetes mellitus, and other obesity related metabolic diseases. In our study, we identified that miR-27b expression was significantly lower during adipogenic differentiation.…”

Section: Discussionmentioning

confidence: 99%

MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL

Hu¹,

Tang²,

Hu³

et al. 2018

Cell Physiol Biochem

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Background/Aims: In this study, the molecular mechanisms of miR-27b and lipoprotein lipase (LPL) that regulate human adipose-derived mesenchymal stem cells (hASCs) adipogenic differentiation were detected. Methods: Microarray analysis was applied to screen for differentially expressed miRNAs and mRNA during hASCs adipocyte differentiation induction. MiR-27b and LPL were found to have abnormal expression. Then, a dual luciferase reporter assay was employed to validate the targeting relationship between miR-27b and LPL. We also utilized qRT-PCR, western blot, cellular immunofluorescence and an oil red O staining assay to analyze the regulation of miR-27b and LPL during adipogenic differentiation. Results: The microarray analysis demonstrated that, during adipogenic differentiation, miR-27b was down-regulated, while LPL was up-regulated but tended to become stable 14 days after induction. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-27b and LPL. After overexpressing and silencing miR-27b, LPL was found to be reversely regulated by miR-27b according to qRT-PCR and western blot. The fat-formation-related biomarkers CCAAT-enhancer binding protein α (c/EBPα) and peroxisome proliferator-activated receptors γ (PPARγ) had decreasing levels after over-expressing miR-27b or knockdown of LPL followed by adipogenic differentiation. Meanwhile, the oil red O staining assay revealed that the accumulation of lipid droplets decreased. There was no change in the expression of c/EBPα, PPARγ, or lipid droplet accumulation when overexpressing miR-27b and LPL. Conclusion: During the adipogenic differentiation of hASCs, miR-27b expression decreased, and LPL expression increased. The abnormal expression of miR-27b and LPL effectively regulated the adipogenic differentiation of hASCs.

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Adipogenic miRNA and meta-signature miRNAs involved in human adipocyte differentiation and obesity

Cited by 99 publications

References 34 publications

Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Tools for Sequence-Based miRNA Target Prediction: What to Choose?

MiR-27b Impairs Adipocyte Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells by Targeting LPL

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