Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Guo, Yuntao; Zhang, Xiuxiu; Huang, Wanlong; Miao, Xiangyang

doi:10.1038/srep44026

Cited by 51 publications

(41 citation statements)

References 59 publications

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“…It is a good indicator of fat deposition in beef cattle. Although RNA-Seq technology has previously been used to explore the transcriptomes of subcutaneous adipose tissue in beef cattle (Guo, Zhang, Huang, & Miao, 2017;Salleh et al, 2017), to our knowledge, the molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of beef cattle based on RNA-Seq technology have not been reported. The high-throughput sequencing of mRNA (RNA-Seq) technology is a powerful tool for quantitative analysis of the expression profile for the whole transcriptomes, and it has become the important components of systems genomic methods (Kadarmideen, 2014;Suravajhala, Kogelman, & Kadarmideen, 2016).…”

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confidence: 99%

“…The high-throughput sequencing of mRNA (RNA-Seq) technology is a powerful tool for quantitative analysis of the expression profile for the whole transcriptomes, and it has become the important components of systems genomic methods (Kadarmideen, 2014;Suravajhala, Kogelman, & Kadarmideen, 2016). Although RNA-Seq technology has previously been used to explore the transcriptomes of subcutaneous adipose tissue in beef cattle (Guo, Zhang, Huang, & Miao, 2017;Salleh et al, 2017), to our knowledge, the molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of beef cattle based on RNA-Seq technology have not been reported.…”

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confidence: 99%

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RNA‐Seq analysis reveals the potential molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle

Liang

Zhao

et al. 2019

Animal Physiology Nutrition

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Daidzein has been reported to be effective in regulating lipid metabolism in animals. However, the molecular mechanisms of daidzein on adipogenesis in beef cattle are not yet reported and the results of daidzein on affecting lipid metabolism in other species have been conflicting. High‐throughput sequencing of mRNA (RNA‐Seq) technology was performed to elucidate the underlying molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle. A total of 893 differentially expressed genes (DEGs) were identified by differential expression analysis, among which 405 genes were upregulated and 488 genes were downregulated. Bioinformatics analysis suggested that these DEGs were significantly enriched to the pathways related to lipid metabolism including ECM–receptor interaction, Glycolysis/Gluconeogenesis and Hedgehog signalling pathway. Daidzein significantly affected the candidate genes (Shh, Pec, Gli, Wnt6, DLK, IGFBP2, ID3 and C/EBPE) related to adipocyte differentiation. Besides, daidzein improved the ability of subcutaneous adipocytes in synthesizing triglycerides by directly using the long‐chain fatty acids and enhanced the efficiency of triglyceride synthesis of subcutaneous adipocytes in Xianan steers. In conclusion, daidzein plays a positive role not only in adipogenic differentiation, but also in triglyceride synthesis in subcutaneous adipose tissue of Xianan beef cattle.

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confidence: 99%

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confidence: 99%

RNA‐Seq analysis reveals the potential molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle

Liang

Zhao

et al. 2019

Animal Physiology Nutrition

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“…Recently, there has been an increase in the utilization of Next Generation Sequencing (NGS) to examine the transcriptome for the identification of differential expression as well as for the opportunity to discover novel transcripts, including new alternative isoforms and miRNAs [14][15][16][17][18][19][20][21][22][23][24][25][26]. Therefore, in the present study, a comparison of the miRNA profiles of the adipose tissue of the Laiwu pig and Large White pig was performed using RNA-seq.…”

Section: Introductionmentioning

confidence: 99%

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

Zhang¹,

Huang²,

Guo³

et al. 2017

Oncotarget

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The domestic pig, an important species in the animal production industry, is also a model system for studying fat deposition and fat-associated diseases in humans. In order to investigate the genetic relationships of the miRNAs that may regulate fat deposition, we performed a genome-wide analysis of miRNAs derived from subcutaneous adipose tissue of Laiwu and Large White pig using RNA-seq. A total of 26,486,906 reads were obtained. Further analysis indicated that 39 known miRNAs and 56 novel miRNAs had significantly differential expression between the two breeds of pigs. Gene Ontology and KEGG pathway analysis revealed that the predicted targets of these differentially expressed miRNAs were involved in several fat-associated pathways, such as the PPAR, MAPK and Wnt signaling pathways. In addition, we found that three miRNAs, ssc-miR-133a-3p, ssc-miR-486 and ssc-miR-1, had an impact on the development of porcine subcutaneous fat through the PPAR signaling pathway. This study provides clues to understand the potential mechanisms of adipogenesis and fat deposition between two different pig breeds. Furthermore, these results may also contribute to the research involving human obesity.

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“…MiR-296 participated in lipoapoptosis regulation by reducing PUMA (also known as BCL2 binding component 3) [47]. The expressional level of miR-874 was lower in Wagyu (high intramuscular fat) than that in Holstein (moderate intramuscular fat) cattle [48].…”

Section: Discussionmentioning

confidence: 99%

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte differentiation by sponging miR-199a-5p to up-regulate CAV-1

Wang

Chen

et al. 2020

Preprint

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Background Local Chinese local pig breeds have better meat quality, such as thinner muscle fiber and higher intramuscular-fat (IMF) content. However, its molecular regulation mechanism has not been discussed in-depth. Studies indicated that long non coding RNAs (lncRNAs) participate in the regulation of muscle and fat development. The expressional differences of lncRNAs in the longissimus dorsi (LD) muscle were identified between Huainan pigs (local Chinese pigs, fat-type, HN) and Large White pigs (lean-type, LW) at 38, 58, and 78 days post conception (dpc).Results In total, 2131 novel lncRNAs were identified in 18 samples, and 291, 305, and 683 differentially expressed lncRNAs (DELs) were found between these two breeds at three stages, respectively. GO and KEGG analysis of the DEL co expressed mRNAs showed that muscle development and energy metabolism were more active at 58 dpc in HN, but at 78 dpc in LW pigs. Muscle cell differentiation and myofibril assembly might contribute to earlier myogenesis and primary-muscle-fiber assembly in HN, and cell proliferation, insulin, and the mitogen-activated protein kinase (MAPK) pathway might contribute to longer proliferation in LW pigs. The PI3K/Akt and cAMP pathways were associated with higher IMF deposition in HN. IMFlnc1 was selected for functional verification, and results indicated that it regulated the expressional level of caveolin-1 (CAV-1) as a competing endogenous RNA (ceRNA) for miR-199a-5p.Conclusion Our data contributed to understanding lncRNAs in porcine-muscle development and IMF deposition, and provided valuable information for improving pig-meat quality.

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Identification and characterization of differentially expressed miRNAs in subcutaneous adipose between Wagyu and Holstein cattle

Cited by 51 publications

References 59 publications

RNA‐Seq analysis reveals the potential molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle

RNA‐Seq analysis reveals the potential molecular mechanisms of daidzein on adipogenesis in subcutaneous adipose tissue of finishing Xianan beef cattle

WITHDRAWN: An integrated analysis of microRNAs involved in fat deposition in different pig breeds

LncRNA IMFlnc1 promotes porcine intramuscular adipocyte differentiation by sponging miR-199a-5p to up-regulate CAV-1

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