Adipokine-cytokine profile of adipocytes of epicardial adipose tissue in ischemic heart disease complicated by visceral obesity

Груздева, О. В.; Викторовна, Груздева Ольга; Бородкина, Д А; Андреевна, Бородкина Дарья; Акбашева, О. Е.; Евгеньевна, Акбашева Ольга; Dileva, Julia A.; Александровна, Дылева Юлия; Антонова, Л. В.; Валерьевна, Антонова Лариса; Матвеева, В. Г.; Геннадьевна, Матвеева Вера; Иванов, Сергей Витальевич; Ivanov, S. V.; Uchasova, Evgenya; Геннадьевна, Учасова Евгения; Белик, Е. В.; Викторовна, Белик Екатерина; Fanaskova, E. V.; Викторовна, Фанаскова Елена; Каретникова, В Н; Николаевна, Каретникова Виктория; Kokov, А. N.; Николаевич, Коков Александр; Barbarash, O. L.; Леонидовна, Барбараш Ольга

doi:10.14341/omet2017438-45

Cited by 6 publications

(4 citation statements)

References 19 publications

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“…Дисбаланс в системе адипокинов способствует развитию адипозопатии и связанных с этим патологическим процессом заболеваний. В частности, аберрантное продуцирование адипокинов коррелирует с развитием и прогрессированием ожирения, атеросклероза, СД2 [7][8][9][10].…”

Section: обоснованиеunclassified

Effect of metformin on transcriptome and adipokinome of adipocytes of local fat deposts of patients with ischemic heart disease

et al. 2023

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BACKGROUND: The information available to date on the effect of metformin on the transcriptome and secretory capacity of adipocytes in human adipose tissue (AT) is scarce and contradictory. The study analyzed whether metformin in vitro modulates gene expression and secretion of adiponectin and leptin in the AT of subcutaneous (SAT), epicardial (EAT) and perivascular (PVAT) localization of patients with ischemic artery disease (IHD).AIM: To assess the effect of metformin at various concentrations (1 and 10 mmol / L) on the level of expression of adiponectin and leptin genes and their content in adipocytes of subcutaneous, epicardial and perivascular AT of patients with IHD.MATERIALS AND METHODS: The study included 134 patients with IHD and indications for direct myocardial revascularization by coronary artery bypass grafting (CABG). During the operation, biopsies of SAT, EAT and PVAT were obtained for 3–5 gram, which served as a source of adipocytes. Isolated adipocytes were cultured for 24 hours with and without metformin (1 and 10 mmol / L). After a day of incubation, the expression of the ADIPOQ and LEP genes and the level of secretion of adiponectin and leptin in the culture medium of adipocytes were determined. Statistical processing was performed using the GraphPad Prism 6 software package (GraphPad Software, La Jolla, CA, USA) and Statistica software, 6.1 (Dell Software, Inc., Round Rock, TX, USA).RESULTS: The adipocytes of the EAT and PVAT of patients with IHD were characterized by an imbalance in the adipokine system, manifested by a low level of ADIPOQ expression and a high LEP expression in comparison with adipocytes of the SAT. Metformin increased the level of ADIPOQ expression and its secretion by adipocytes regardless of their location, while a low concentration (1 mmol / L) in adipocytes of EAT had a stronger effect compared to 10 mmol / L. Metformin had a multidirectional effect on the level of leptin in adipocytes, which depended on their localization: both in low (1 mmol / L) and high concentrations (10 mmol / L), the drug reduced the level of LEP expression and protein secretion in the culture medium of adipocytes of the SAT. EAT had no significant effect on adipocytes. In PVAT, metformin increased the expression and secretion of leptin regardless of concentration.CONCLUSION: Metformin has a direct effect on adipocytes in SAT, EAT, and PVAT and is able to modulate their activity, which is a promising strategy for maintaining the balance of adipokines in AT, especially epicardial and perivascular localization.

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Section: обоснованиеunclassified

Effect of metformin on transcriptome and adipokinome of adipocytes of local fat deposts of patients with ischemic heart disease

et al. 2023

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“…Большой объем жировой ткани сам по себе является постоянным источником провоспалительных цитокинов, синтезируемых как самими адипоцитами, так и мигрировавшими в жировую ткань макрофагами, что приводит, как уже упоминалось, к формированию и поддержанию в организме хронического вялотекущего воспалительного процесса. В свою очередь патологическая секреция адипокинов в жировой ткани (ИЛ-1, ИЛ-6, TNFα) в сочетании с увеличением С-реактивного белка, лептин-адипонектинового отношения и снижением содержания протективных факторов (адипонектина, противоспалительного цитокина ИЛ-10) сопровождаются ухудшением иммунного ответа и неблагоприятным воздействием на все органы и ткани, в том числе на легочную паренхиму и бронхи [26,27]. Установлено, что увеличение провоспалительных биомаркеров находится в прямой зависимости от степени выраженности ОЖ.…”

Section: патофизиология тяжелого течения коронавирусной инфекции на фоне ожиренияunclassified

Coronavirus Infection an Obese Patient (Literature Review)

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Pimankina

2021

Arhivʺ vnutrennej mediciny

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In the modern world the problem of obesity in combination with new coronavirus infection has acquired a special danger. On the one hand, the prevalence of obesity among the population is steadily increasing, on the other-it has been proven that obese people are among the most vulnerable in terms of increased risk of infection and a serious prognosis. This is due to the presence and peculiarities of the development of various pathological mechanisms in this category of patients. These include: high expression of angiotensin-converting enzyme 2, a high probability of a «cytokine storm» developing, maintenance of a chronic inflammatory process in adipose tissue, changes in the activity of Dipeptidyl peptidase-4 enzyme. All these processes lead to an aggravation of metabolic disorders in adipose tissue and violation of immune protection. The world medical practice in the fight against the COVID-19 pandemic shows that patients with coronavirus infection against the background of obesity more often need hospitalization in intensive care units and connection to artificial ventilation equipment. Currently, many features of the course of coronavirus infection against the background of obesity have been identified and continue to be studied. These include: the presence of severe respiratory failure, a high risk of developing respiratory distress syndrome, thrombosis and thromboembolic complications, as well as worsening of the course of chronic cardiovascular diseases. All this eventually leads to the development of severe multiple organ failure, which is often the cause of death in this category of patients.

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“…Відносна кількість адипоцитів становить 267 клітин на 1 мм 2 , що достовірно більше, ніж у групі ВКД, і достовірно менше, ніж у контролі (рис. [2][3][4].…”

Section: т а б л и ц я 1 характеристика експериментальних груп тваринunclassified

“…Причиною такого явища може бути зміна щільності ліпідів, якими заповнений адипоцит, у результаті біохімічних перетворень. Для остаточного з'ясування причини, яка зумовлює збільшення розмірів адипоцитів без зростання маси жирової тканини, необхідні подальші імуногістохімічні та біохімічні дослідження [2].…”

Section: т а б л и ц я 1 характеристика експериментальних груп тваринunclassified

Morphofunctional state of rat visceral white adipose tissue under obesity development and silver nanoparticles administration

et al. 2020

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There was studied the morphofunctional state of the visceral white adipose tissue of rats with obesity and after introduction of silver salt solution and silver nanoparticles solution. In particular, we analyzed the morphofunctional changes in the visceral white adipose tissue of rats on a high-calorie diet, as well as the effects of the silver nitrate solution and the silver nanoparticles solution at a dose of 1 mg / 1 kg body weight and 0.5 mg / 1 kg body weight on the morphofunctional state of the visceral white adipose tissue of rats on a high-calorie diet. There were established morphofunctional changes in cells of the rats white adipose tissue on the high-calorie diet and with the introduction of silver preparations in different concentrations. The condition of the adipose tissue was evaluated on the basis of the following parameters: the relative mass of the visceral fat, the cross-sectional area of adipocytes, the relative number of adipocytes per unit area. There were compared effects of administration to animals with obesity of the silver nitrate solution at a dose of 1 mg / 1 kg body weight and 0.5 mg / 1 kg body weight, as well as a of the silver nanoparticles solution at a dose 1 mg / 1 kg body weight and 0.5 mg / 1 kg of body weight. All the above silver preparations reduced the manifestations of obesity in animals which were maintained in a high-calorie diet. In addition to the form of the silver preparation (silver nitrate solution or silver nanoparticle solution), its concentration is important. The highest efficiency was shown after the administration of the silver nanoparticles solution at a dose 0.5 mg / 1 kg of body weight. In rats of this experimental group, the studied parameters not only reached the control values, but they also exceeded them, which indicates expressed influence of the drug on the obesity manifestations. In addition, the rats showed signs of fat depletion.

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Adipokine-cytokine profile of adipocytes of epicardial adipose tissue in ischemic heart disease complicated by visceral obesity

Cited by 6 publications

References 19 publications

Effect of metformin on transcriptome and adipokinome of adipocytes of local fat deposts of patients with ischemic heart disease

Effect of metformin on transcriptome and adipokinome of adipocytes of local fat deposts of patients with ischemic heart disease

Coronavirus Infection an Obese Patient (Literature Review)

Morphofunctional state of rat visceral white adipose tissue under obesity development and silver nanoparticles administration

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