Adipose-derived stem cells as a new therapeutic modality for ageing skin

Abstract

Stem cells are undifferentiated cells, which have the important properties of self-renewal and differentiation. Adipose-derived stem cells (ADSC) have relative advantages in accessibility and abundance compared to other kinds of stem cells. Regeneration therapy using ADSC has received attention in the treatment of various dermatologic diseases. In previous studies, ADSC were shown to have antioxidant, whitening and wound-healing effects in the skin through secretion of growth factors and by activating fibroblasts. In this study, we investigated whether ADSC could be used as an anti-ageing therapy, especially by dermal collagen synthesis and angiogenesis. Subcutaneous injection of ADSC significantly increased collagen synthesis in hairless mice, and dermal thickness, collagen density and fibroblast number also increased. In addition, procollagen type I protein and mRNA expression increased, which accounts for the increased dermal collagen density. Angiogenesis, which was visualized by CD31 and NG2 immunofluorescence stains, also increased in ADSC-treated skin. Our results suggest that ADSC therapy may be useful in ageing skin. Its effects are mainly mediated by stimulating collagen synthesis in dermal fibroblasts and increasing angiogenesis.

Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

“…[3][4][5] The in vitro expansion of ADSCs in culture medium is an important approach to obtain substantial cells before cell transplantation. However, it was reported that the in vitro cultured cells produced more reactive oxygen species (ROS) when compared to the in vivo conditions.…”

Hypoxic Culturing Enhances the Wound-Healing Potential of Adipose-Derived Stem Cells

“…In addition to our study, other groups have demonstrated the wound-healing effect of ASCs and their mechanism of action, and found that locally transplanted ASCs accelerate wound healing not only by differentiation and vasculogenesis but also by paracrine factors. 6,17 Induced pro-angiogenic potential of ASCs by hypoxia has been reported that the transplantation of ASCs cultured in hypoxia significantly increased the local blood flow and angiogenic gene expression was partially improved by hypoxia preconditioning. 18,19 Incubation under hypoxic conditions enhanced the proliferation of ASCs in either the presence or absence of serum.…”

Estabelecimento de cultura de células de pluripotência induzida a partir de células tronco derivadas do tecido adiposo de coelhos

“…Entre as fontes de células tronco mesenquimais, o tecido adiposo se destaca pela acessibilidade e segurança na coleta e abundância de células isoladas (HOUSMAN et al, 2002;CHERUBINO et al, 2010;DU et al, 2010;KIM et al, 2011;LAM;LONGAKER, 2012). Cada coleta resulta em aproximadamente 100 vezes mais células do que a coleta a partir da medula óssea, (DEY; EVANS, 2011), sendo o processo bem menos doloroso para o indivíduo (SACHS et al, 2012).…”