Vitamin C promotes the proliferation of human adipose-derived stem cells via p53-p21 pathway

Abstract

ABSTRACT. Although adipose-derived stem cells (ADSCs) have demonstrated a promising potential for the applications of cell-based therapy and regenerative medicine, excessive reactive oxygen species (ROS) are harmful to ADSCs cell survival and proliferation. Vitamin C is an important antioxidant, and is often added into culture media as an essential micronutrient. However, its roles on the proliferation of human ADSCs have not been studied. Therefore, in this study, human ADSCs were isolated, and detected by flow cytometry for the analysis of their cell surface antigens. Cell proliferation and cell cycle progression were measured with cell counting kit-8 assay and flow cytometry, respectively. Western blotting was used to detect the expression levels of cyclin E1, p53, p21, and CDK2 proteins. The effect of vitamin C pretreatment on the production of hydrogen peroxide (H 2 O 2 )-mediated ROS in the ADSCs was evaluated by flow cytometry. Our results indicated that vitamin C treatment significantly increased cell proliferation, and changed the cell cycle distribution of ADSCs by decreasing the percentage of G 1 phase, and concurrently increased the percentage of S and G 2 /M phase. Western blot analysis indicated that vitamin C treatment up-regulated the expression levels of cyclin E1 and CDK2, but down-regulated p53 and p21 proteins expression, which contributed to cell proliferation and cell cycle progression. Vitamin C pretreatment significantly reduced the production of H 2 O 2 -induced ROS in the ADSCs. These findings suggest that vitamin C can promote the proliferation and cell cycle progression in the ADSCs possibly through regulation of p53-p21 signal pathway.

Vitamin C improves the therapeutic potential of human amniotic epithelial cells in premature ovarian insufficiency disease

“…1). In accordance with previous studies, VC treatment was demonstrated to increase the proliferation rate in various kinds of mesenchymal stem cells [29,32]. VPA was reported to accelerate the proliferation and differentiation of neural stem cells but inhibit the propagation of mesenchymal stem cells and epithelial cells [33][34][35].…”

“…2c). Previous studies indicated that VC promoted the cell cycle by upregulating cyclin E1 and CDK2 and downregulating p53 and p21 [32]. The life span and telomere expression of hAECs was extended by 50 μg/ml VC (Fig.…”

Accelerated corneal endothelial cell loss in two patients with granulomatosis with polyangiitis following phacoemulsification

“…Perioperative topical ascorbic acid (AA) has been shown preventive effects of corneal endothelial dysfunction in high-risk patients undergoing phacoemulsification [16]. AA has shown promoting proliferation effects in other human cells [25,26]. In vitro evidence also shed light on the proliferative effects of AA on corneal endothelial cells [27].…”

Unravelling the developmental roadmap towards human brown adipose tissue

“…At D4, paraxial mesoderm-like precursors became brown adipocyte precursor cells upon treatment for 48h with insulin, FGF2, Chiron, and LDN-193189 (a BMP inhibitor). Also, ascorbic acid was used to promote proliferation 26 . Following adipocyte precursor formation, the cells were treated with adipogenic induction between D6-D8, requiring culturing cells in DMEM/HAMF12 medium supplemented with triiodothyronine (T3), dexamethasone, 3-isobutyl-1-methylxanthine (IBMX), biotin, pantothenate, insulin, rosiglitazone, ascorbic acid and serum.…”