Adipose-derived stem cells for breast reconstruction after breast surgery – preliminary results

Tsekouras, Anastasios; Mantas, Dimitrios; Tsilimigras, Diamantis I.; Ntanasis‐Stathopoulos, Ioannis; Kontos, Michael C.; Zografos, George

doi:10.1080/23320885.2017.1316201

Cited by 15 publications

(17 citation statements)

References 26 publications

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“…Due to the abundance of adipose tissue, as well as the minimally invasive procedures required to collect it, ASCs are a celebrated approach for tissue engineering and regenerative medicine. For example, lipoaspirate preparations may be “enriched” by the addition of ASCs to improve graft volume retention (204, 403). However, the findings described below suggest that caution may be advised in use of ASCs in patients with a history of cancer.…”

Section: Microenvironmental Links Between Adipose Tissue and Cancermentioning

confidence: 99%

Contribution of Adipose Tissue to Development of Cancer

Cozzo

Fuller

Makowski

2017

Comprehensive Physiology

168

130

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Solid tumor growth and metastasis require the interaction of tumor cells with the surrounding tissue, leading to a view of tumors as tissue-level phenomena rather than exclusively cell-intrinsic anomalies. Due to the ubiquitous nature of adipose tissue, many types of solid tumors grow in proximate or direct contact with adipocytes and adipose-associated stromal and vascular components, such as fibroblasts and other connective tissue cells, stem and progenitor cells, endothelial cells, innate and adaptive immune cells, and extracellular signaling and matrix components. Excess adiposity in obesity both increases risk of cancer development and negatively influences prognosis in several cancer types, in part due to interaction with adipose tissue cell populations. Herein, we review the cellular and noncellular constituents of the adipose “organ,” and discuss the mechanisms by which these varied microenvironmental components contribute to tumor development, with special emphasis on obesity. Due to the prevalence of breast and prostate cancers in the United States, their close anatomical proximity to adipose tissue depots, and their complex epidemiologic associations with obesity, we particularly highlight research addressing the contribution of adipose tissue to the initiation and progression of these cancer types. Obesity dramatically modifies the adipose tissue microenvironment in numerous ways, including induction of fibrosis and angiogenesis, increased stem cell abundance, and expansion of proinflammatory immune cells. As many of these changes also resemble shifts observed within the tumor microenvironment, proximity to adipose tissue may present a hospitable environment to developing tumors, providing a critical link between adiposity and tumorigenesis.

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Section: Microenvironmental Links Between Adipose Tissue and Cancermentioning

confidence: 99%

Contribution of Adipose Tissue to Development of Cancer

Cozzo

Fuller

Makowski

2017

Comprehensive Physiology

168

130

View full text Add to dashboard Cite

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“…Two fractions can be obtained from the adipose tissue isolate based upon cell density. The separation can be enhanced by density-based centrifugation employing compounds such as Percoll, gravity-based phase separation, and filtration that leaves mature adipocyte on the surface and the aqueous fraction at the bottom [9]. Filtration will be more precise as it can specifically isolate cells based on size, while centrifugation will pellet all the cells [10].…”

Section: Isolation Of Svfmentioning

confidence: 99%

“…Filtration will be more precise as it can specifically isolate cells based on size, while centrifugation will pellet all the cells [10]. The aqueous fraction is where the SVF is found, which is the source of ADSCs [9]. There are two hurdles ofconventional isolation.…”

Section: Isolation Of Svfmentioning

confidence: 99%

“…There are two hurdles ofconventional isolation. Firstly, conventional isolation not only requires the infrastructure and equipment that are not available to every healthcare facility, but also, because of the presence of collagenase in the product, which is required to process the crude samples, may provide a regulatory hurdle [11,9]. A non-enzymatic methods of SVF isolation, may be mechanical agitation, which bases on breaking down the adipose tissue allowing the release stromal cells.…”

Section: Isolation Of Svfmentioning

confidence: 99%

“…In fact, vascular endothelial growth factor-transduced ADSCs showed high capillary density, improving neovascularization [7]. ADSCs are effective not only in cosmetic breast augmentation [8], where their use proved to be highly effective in a study involving 40 patients, but can also be harnessed in breast reconstruction following breast resection [9]. Despite its significant advantages in the field of tissue augmentation, ADSC graft enrichment needs to be used carefully after breast cancer as it may increase the proliferation and metastasis of tumour cells [10,11].…”

Section: Fat Grafting and Tumorigenesismentioning

confidence: 99%

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Current clinical applications of adipose-derived stem cells in humans and animals

Dompe

Wąsiatycz

Mozdziak

et al. 2019

Medical Journal of Cell Biology

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Adipose derived stem cells are a type of mesenchymal stem cell that, because of their straightforward isolation procedure and ready availability, have been intensively studied in the recent years regarding their possible clinical applications. Additionally, ADSCs have the ability to differentiate into tri-germ lineages, as well as exhibit paracrine activity. Their capacity to differentiate into many different cell lineages such as osteocytes, adipocytes, neural cells, vascular endothelial cells, cardiomyocytes, pancreatic cells, and hepatocytes, has granted them a significant place in consideration for tissue engineering and for their application in regenerative medicine. Moreover, their endocrine activity has a great impact upon therapies as it grants immunosuppressive properties and low immunogenicity.Running title: Clinical applications of ADSCs

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Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

Sutthiwanjampa

Shin

Ryu

et al. 2021

Bioengineering & Transla Med

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Medical devices made from poly(dimethylsiloxane) (PDMS)‐based silicone implants have been broadly used owing to their inert properties, biocompatibility, and low toxicity. However, long‐term implantation is usually associated with complications, such as capsular contracture due to excessive local inflammatory response, subsequently requiring implant removal. Therefore, modification of the silicone surface to reduce a risk of capsular contracture has attracted increasing attention. Human adipose‐derived stem cells (hASCs) are known to provide potentially therapeutic applications for tissue engineering, regenerative medicine, and reconstructive surgery. Herein, hASCs coating on a PDMS (hASC‐PDMS) or itaconic acid (IA)‐conjugated PDMS (hASC‐IA‐PDMS) surface is examined to determine its biocompatibility for reducing capsular contracture on the PDMS surface. In vitro cell cytotoxicity evaluation showed that hASCs on IA‐PDMS exhibit higher cell viability than hASCs on PDMS. A lower release of proinflammatory cytokines is observed in hASC‐PDMS and hASC‐IA‐PDMS compared to the cells on plate. Multiple factors, including in vivo mRNA expression levels of cytokines related to fibrosis; number of inflammatory cells; number of macrophages and myofibroblasts; capsule thickness; and collagen density following implantation in rats for 60 days, indicate that incorporated coating hASCs on PDMSs most effectively reduces capsular contracture. This study demonstrates the potential of hASCs coating for the modification of PDMS surfaces in enhancing surface biocompatibility for reducing capsular contracture of PDMS‐based medical devices.

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Adipose-derived stem cells for breast reconstruction after breast surgery – preliminary results

Cited by 15 publications

References 26 publications

Contribution of Adipose Tissue to Development of Cancer

Contribution of Adipose Tissue to Development of Cancer

Current clinical applications of adipose-derived stem cells in humans and animals

Assessment of human adipose‐derived stem cell on surface‐modified silicone implant to reduce capsular contracture formation

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