Adipose-Derived Stem Cells Therapy for Radiation-Induced Skin Injury

Huayllani, María T.; Ruiz‐Garcia, Henry; Boczar, Daniel; Ávila, Francisco R.; Lu, Xiaona; Rinker, Brian; Moran, Steven L.; Sarabia-Estrada, Rachel; Quiñones‐Hinojosa, Alfredo; Forte, Antonio J.

doi:10.1097/sap.0000000000003039

Cited by 7 publications

(5 citation statements)

References 32 publications

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“…93 There is evidence for positive effects of ADSC on radiation-induced skin injury. 94 Some complex defects may require fat grafting and there is some evidence that MSC can enhance fat graft take. 95 The use of such therapies have also been investigated in conjunction with other techniques, such as platelet rich plasma (PRP) 96 , and combination with an acellular dermal matrix.…”

Section: Wider Clinical Applications For Stem Cell Derived Therapiesmentioning

confidence: 99%

Innovations in Stem Cell Therapy for Diabetic Wound Healing

Yue

Cheema

et al. 2023

Advances in Wound Care

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Significance:The global burden of diabetic wounds, particularly diabetic foot ulcers, continues to have large economic and social impact throughout the world. Current strategies are not sufficient to overcome this burden of disease. Finding newer, more advanced regenerative cell and tissue-based strategies to reduce morbidity remains paramount.Recent Advances: Recent advances in stem cell therapies are discussed. We also highlight the practical issues of translating these advancing technologies into the clinical setting.Critical Issues: We discuss the use of somatic and induced pluripotent stem cells and the stromal vascular fraction, as well as innovations including the use of 3D bioprinting of skin. We also explore related issues of using regenerative techniques in clinical practice, including the current regulatory landscape and translatability of in vivo research.Future Directions: Advances in stem cell manipulation showcase the best therapeutic resources available to enhance mechanisms of wound healing such as angiogenesis, cell proliferation, and collagen synthesis; potential methods include changing the scaffold microenvironment including relative oxygen tension, and the use of gene modification and nanotechnology. Secretome engineering, particularly the use of extracellular vesicles may be another potential cell-derived therapeutic that may enable use of cell-free translational therapy. Wider clinical applications for stem cell derived therapies .

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Section: Wider Clinical Applications For Stem Cell Derived Therapiesmentioning

confidence: 99%

Innovations in Stem Cell Therapy for Diabetic Wound Healing

Yue

Cheema

et al. 2023

Advances in Wound Care

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“…Some new studies regarding stem cells and tissue engineering are also emerging, 7,8 but they are still far from clinical transformation and have conflicting findings. For this reason, there is an urgent need to find a suitable, efficient and clinically applicable means to repair RSI 9 …”

Section: Introductionmentioning

confidence: 99%

“…For this reason, there is an urgent need to find a suitable, efficient and clinically applicable means to repair RSI. 9 The application of platelet-rich plasma (PRP) based on autologous blood-derived biomaterials plays a role in various regenerative medicine treatments, such as orthopaedic surgery, cardiac surgery and maxillofacial surgery. 10,11 Due to the high concentration of platelets, PRP can promote tissue synthesis, and secretion of up to 300 proteins (including signalling proteins, membrane proteins and cytoskeleton regulatory proteins), and a variety of biological activities, which plays a key role in maintaining haemostasis, promoting tissue repair and angiogenesis and regulating the inflammatory responses.…”

mentioning

confidence: 99%

Autologous i‐PRF promotes healing of radiation‐induced skin injury

Tian

Jingcheng

Zhong

et al. 2023

Wound Repair Regeneration

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Skin, as an exposed tissue, often suffers damage after exposure to radiotherapy and accidental events, which may lead to the formation of chronic refractory wounds.However, effective treatment options are usually limited for severe radiation-induced skin injury (RSI). Platelet-rich plasma (PRP) has been identified to promote wound healing, but whether a new generation of blood-derived biomaterial, injectable platelet-rich fibrin (i-PRF), is effective in repairing RSI remains unclear. In this study, blood was drawn from humans and Sprague-Dawley rats to prepare PRP and i-PRF, and the regenerative functions of PRP and i-PRF were investigated by exposing the dorsal skin of SD rats to local radiation (45 Gy) and exposing HDF-α cells and human umbilical vein endothelial cells (HUVECs) cells to X-rays (10 Gy). The healing effect of i-PRF on RSI was analysed by tube formation assay, cell migration and apoptosis assays, ROS assay, wound healing assay, histological characterisation and immunostaining. The results showed that exposure to high doses of radiation reduced cell viability, increased ROS levels and induced cell apoptosis, thereby causing dorsal trauma of rats. However, both PRP and i-PRF could resisted RSI, and they were capable of reducing inflammation and promoting angiogenesis and vascular regeneration. i-PRF has a higher concentration of platelets and platelet-derived growth factors, which has a more convenient preparation method and better repair effect and possesses a good application prospect for the repair of RSI.

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“…In recent years, mesenchymal stem cells (MSCs) have come to the fore for tissue regeneration and wound repair 9,10 . Numerous preclinical studies report the positive effects of MSCs on wound healing by promoting angiogenesis, regulating extracellular matrix (ECM), and accelerating wound closure 11,12 . Compared to other kinds of MSCs, adipose-derived mesenchymal stem cells (ADSCs) are widely available and can secrete most of the cytokines related to wound repair 13 .…”

mentioning

confidence: 99%

“…9,10 Numerous preclinical studies report the positive effects of MSCs on wound healing by promoting angiogenesis, regulating extracellular matrix (ECM), and accelerating wound closure. 11,12 Compared to other kinds of MSCs, adipose-derived mesenchymal stem cells (ADSCs) are widely available and can secrete most of the cytokines related to wound repair. 13 Moreover, the positive effect of ADSCs therapy on wound healing is realized through the paracrine effect mediated by ADSCs secretory factors, exosomes (ADSC-exos).…”

mentioning

confidence: 99%

The Optimal Effective Dose of Adipose-Derived Stem Cell Exosomes in Wound Healing

Kemaloğlu,

Dursun,

Yay

et al. 2024

Ann Plast Surg

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Introduction Although the effect of adipose-derived mesenchymal stem cell exosomes (ADSC-exos) on wound healing with different doses are shown in various studies, efficient and sufficient doses of ADSC-exos are still unknown. The study aimed to determine the optimal dose of ADSC-exos in wound healing. Methods The 45 Sprague-Dawley rats were randomly divided into five groups, with seven animals in each. After dorsal circular defects were created, each wound was injected as follows: group 1: saline, group 2: 10 μg/mL of ADSC-exos, group 3: 100 μg/mL of ADSC-exos, group 4: 200 μg/mL of ADSC-exos, and group 5: 400 μg/mL of ADSC-exos. The effects of ADSC-exos on epithelization, angiogenesis, and collagen formation were analyzed macroscopically, histopathologically, and immunohistochemically on day 14. Results A total of 200 μg/mL and 400 μg/mL ADSC-exos groups had higher epithelial tongue length, epithelial tongue area, and angiogenesis scores than the other groups. Although there was no statistical difference in fibrosis scores among groups, collagen fibers were becoming well-organized as the ADSC-exos doses increased. While the wound area was clinically smaller in the 200 μg/mL ADSC-exos group, there was no statistically significant difference among groups on day 14. Conclusions A total of 200 μg/mL of ADSC-exos was found to be the adequate and effective dose for re-epithelialization and angiogenesis in cutaneous wound healing. Moreover, the collagen density increased with a more regular pattern in the 200 μg/mL group, which can be important in scar regulation.

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Adipose-Derived Stem Cells Therapy for Radiation-Induced Skin Injury

Cited by 7 publications

References 32 publications

Innovations in Stem Cell Therapy for Diabetic Wound Healing

Innovations in Stem Cell Therapy for Diabetic Wound Healing

Autologous i‐PRF promotes healing of radiation‐induced skin injury

The Optimal Effective Dose of Adipose-Derived Stem Cell Exosomes in Wound Healing

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