Adipose-Derived Stromal Vascular Fraction Differentially Expands Breast Progenitors in Tissue Adjacent to Tumors Compared to Healthy Breast Tissue

Abstract

Supplemental Digital Content is available in the text.

Stem cells for cartilage repair: what exactly were used for treatment, cultured adipose-derived stem cells or the unexpanded stromal vascular fraction?

“…Researches, reviewers and editors are encouraged to look closely at the true identity of the cells using the characteristics put forward through the IFATS and ISCT joint statement. While there are published reports declaring the use of ASCs but in fact what were used was the SVF 5 , other authors using the same material as used in this study refer this correctly as the SVF rather than ASCs 6,7 in their title and abstract. We cannot but emphasize the importance of having an accurate title and abstract as these highlights the objectives, methods, results and conclusion of the study.…”

Current clinical applications of adipose-derived stem cells in humans and animals

“…Following washing and shaking of the lipoaspirate, centrifugation is the next important step, required in both methods for concentration of stem cells. The agitation isolation technique reduces the regulatory burden compared to collagenase-based isolation, but it also prevents contamination when the cells are immediately administrated to a patient [12]. However, ADSCs isolated using agitation remain tightly bound by collagen-based extracellular matrix, resulting in a poor cell yield is much lower compared to conventional collagenase isolation [12,13].…”

“…The agitation isolation technique reduces the regulatory burden compared to collagenase-based isolation, but it also prevents contamination when the cells are immediately administrated to a patient [12]. However, ADSCs isolated using agitation remain tightly bound by collagen-based extracellular matrix, resulting in a poor cell yield is much lower compared to conventional collagenase isolation [12,13]. Through adherence to cell culture dishes, ADSCs can be isolated and secondly expanded in vitro [1].…”

“…Despite its significant advantages in the field of tissue augmentation, ADSC graft enrichment needs to be used carefully after breast cancer as it may increase the proliferation and metastasis of tumour cells [10,11]. Furthermore, SVF enrichment employed in breast augmentation was observed to be more efficient in cases following mastectomy rather than in cosmetic corrections [12]. Additionally, studies showed that ADSCs have an effect on active, but not on resting cancer cells making it important on a precautionary basis, to perform a reconstructive therapy with ASC-augmented fat only after complete establishment of cancer remission [13,14].…”

Hydrogels in adipose tissue engineering—Potential application in post‐mastectomy breast regeneration

“…ADSCs are rapidly becoming the gold standard as a cell source for tissue engineering and regenerative medicine. They are contained within the SVF of adipose tissue and are hypothesised to improve wound healing, tissue regeneration, and graft retention (Chatterjee, Laliberte, Blelloch, Ratanshi, & Safneck, ). According to the International Federation for Adipose Therapeutics and Science and International Society for Cellular Therapy joint statement on ADSCs, these cells are identified phenotypically as a CD45−, CD235a−, CD31−, and CD34+ population.…”