Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes

Compera, Nina; Atwell, Scott; Wirth, Johannes; Toerne, Christine von; Hauck, Stefanie M.; Meier, Matthias

doi:10.1039/d2lc00245k

Cited by 13 publications

(6 citation statements)

References 54 publications

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“…However, how this method compares against other known scaffold and scaffold-free techniques of 3D spheroid formation has not yet been investigated. Thus, the development of techniques enabling quicker turnaround time in screening (e.g., hydrogels with an embossed surface for the mass production of human ADSC-derived spheroids [ 169 ]) or other screening models developed by 3D printing (e.g., adipose microtissue-on-chip [ 170 ]) are essential to ensure a smooth flow driving drug discovery.…”

Section: Potential Techniques To Study Lipedemamentioning

confidence: 99%

Lipedema: Insights into Morphology, Pathophysiology, and Challenges

2022

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Lipedema is an adipofascial disorder that almost exclusively affects women. Lipedema leads to chronic pain, swelling, and other discomforts due to the bilateral and asymmetrical expansion of subcutaneous adipose tissue. Although various distinctive morphological characteristics, such as the hyperproliferation of fat cells, fibrosis, and inflammation, have been characterized in the progression of lipedema, the mechanisms underlying these changes have not yet been fully investigated. In addition, it is challenging to reduce the excessive fat in lipedema patients using conventional weight-loss techniques, such as lifestyle (diet and exercise) changes, bariatric surgery, and pharmacological interventions. Therefore, lipedema patients also go through additional psychosocial distress in the absence of permanent treatment. Research to understand the pathology of lipedema is still in its infancy, but promising markers derived from exosome, cytokine, lipidomic, and metabolomic profiling studies suggest a condition distinct from obesity and lymphedema. Although genetics seems to be a substantial cause of lipedema, due to the small number of patients involved in such studies, the extrapolation of data at a broader scale is challenging. With the current lack of etiology-guided treatments for lipedema, the discovery of new promising biomarkers could provide potential solutions to combat this complex disease. This review aims to address the morphological phenotype of lipedema fat, as well as its unclear pathophysiology, with a primary emphasis on excessive interstitial fluid, extracellular matrix remodeling, and lymphatic and vasculature dysfunction. The potential mechanisms, genetic implications, and proposed biomarkers for lipedema are further discussed in detail. Finally, we mention the challenges related to lipedema and emphasize the prospects of technological interventions to benefit the lipedema community in the future.

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Section: Potential Techniques To Study Lipedemamentioning

confidence: 99%

Lipedema: Insights into Morphology, Pathophysiology, and Challenges

2022

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“…The platform features a vasculature-like perfusion that integrates 3D tissues comprising all major WAT-associated cellular components (Figure A) and recapitulates pivotal WAT functions, such as energy storage and mobilization as well as endocrine and immunomodulatory activities. An alternative platform automated the formation, long-term culture, and retrieval of 3D adipose microtissues . The adipose microtissues on the chip platform were then used to periodically simulate food intake by alternating the glucose level in the cell-feeding media every 6 h over the course of 1 week.…”

Section: Tissue Organ Organoids and Whole Organismsmentioning

confidence: 99%

“…An alternative platform automated the formation, long-term culture, and retrieval of 3D adipose microtissues. 139 The adipose microtissues on the chip platform were then used to periodically simulate food intake by alternating the glucose level in the cell-feeding media every 6 h over the course of 1 week. Coupling mass spectrometry to the cell culture platform allowed one to obtain robust and complex proteomes with over 1800 identified proteins and revealed unique protein profiles of the proteomes of adipocytes under low/high glucose conditions.…”

Section: ■ Bioassay and Diagnostic Applicationsmentioning

confidence: 99%

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Aubry

Lee

2023

Anal. Chem.

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“…Fat tissue, as a major energy reserve, will contribute to obesity due to the imbalance between energy intake and expenditure, and its associated comorbidities present a looming challenge to healthcare delivery throughout the world 165 , 166 . The interaction of immune cells and adipocytes may lead to chronic low-grade inflammation, which will then result in insulin resistance.…”

Section: The Application Of Single-organ-on-a-chip In Drug Evaluationmentioning

confidence: 99%

Organ-on-a-chip meets artificial intelligence in drug evaluation

Deng,

Li,

Cao

et al. 2023

Theranostics

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Drug evaluation has always been an important area of research in the pharmaceutical industry. However, animal welfare protection and other shortcomings of traditional drug development models pose obstacles and challenges to drug evaluation. Organ-on-a-chip (OoC) technology, which simulates human organs on a chip of the physiological environment and functionality, and with high fidelity reproduction organ-level of physiology or pathophysiology, exhibits great promise for innovating the drug development pipeline. Meanwhile, the advancement in artificial intelligence (AI) provides more improvements for the design and data processing of OoCs. Here, we review the current progress that has been made to generate OoC platforms, and how human single and multi-OoCs have been used in applications, including drug testing, disease modeling, and personalized medicine. Moreover, we discuss issues facing the field, such as large data processing and reproducibility, and point to the integration of OoCs and AI in data analysis and automation, which is of great benefit in future drug evaluation. Finally, we look forward to the opportunities and challenges faced by the coupling of OoCs and AI. In summary, advancements in OoCs development, and future combinations with AI, will eventually break the current state of drug evaluation.

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Adipose microtissue-on-chip: a 3D cell culture platform for differentiation, stimulation, and proteomic analysis of human adipocytes

Abstract: In this study, we developed a microfluidic large-scale integration chip platform to automate longitudinal 3D cell culture studies. Proteome analysis of on chip cultured adipocytes under changing glucose conditions revealed metabolic stress profiles.

Cited by 13 publications

References 54 publications

Lipedema: Insights into Morphology, Pathophysiology, and Challenges

Lipedema: Insights into Morphology, Pathophysiology, and Challenges

Advances in Microfluidics: Technical Innovations and Applications in Diagnostics and Therapeutics

Organ-on-a-chip meets artificial intelligence in drug evaluation

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