Adipose Tissue-Derived Stem Cell Sheet Application for Tissue Healing In Vivo: A Systematic Review

Abstract

Adipose tissue-derived stem cells (ASCs) are known to be tissue-healing promoters due to their cellular plasticity and secretion of paracrine factors. Cultured ASC sheets provide a novel method of ASC application and can retain ASCs at the targeted tissue. The purpose of this systematic review is to evaluate preclinical studies using ASC sheet transplantation therapy for promoting tissue healing. First, we searched databases to identify studies of ASC sheet therapy in different experimental animal models, and then determined the quality score of studies using SYRCLE's risk bias tool. A total of 18 included studies examined the role of ASC sheets on tissue healing and function in models for myocardial infarction, dilated cardiomyopathy, full-thickness skin wounds, hind limb ischemia, esophageal strictures, and oral ulcers. ASC sheet application after myocardial infarction improved survival rate, cardiac function, and capillary density and reduced the extent of fibrosis. Application of ASC sheets to a full-thickness skin wound decreased the wound size and stimulated wound maturation. In the hind limb ischemia model, ASC sheet application improved limb perfusion and capillary density, and decreased the amount of ischemic tissue and inflammation. ASC sheet application to mucosal wounds of the digestive tract accelerated wound healing and decreased the degree of stricture and fibrosis. Taken together, transplanted ASC sheets had a positive effect on tissue healing and reconstruction in these preclinical studies. The reported favorable effects of ASC sheet therapy in various tissue healing applications may be implemented in future translational studies. It is suggested that future preclinical animal model studies of ASC sheet therapy should concern standardization of culture techniques and investigate the mechanisms of action. In addition, clearly indicated experimental setups according to the SYRCLE's guidelines should improve study quality and validity.

The Influence of Cell Culture Density on the Cytotoxicity of Adipose-Derived Stem Cells Induced by L-Ascorbic Acid-2-Phosphate

“…Our data highlights the benefit of co-administration of A2-P and NAC for ASC culture. The information is particularly useful for ASC sheet fabrication, where a high concentration of A2-P is usually required to enhance ECM deposition 17,34 .…”

Extracorporeal Shock Wave‐Supported Adipose‐Derived Fresh Stromal Vascular Fraction Preserved Left Ventricular (LV) Function and Inhibited LV Remodeling in Acute Myocardial Infarction in Rat

“…Most experimental or clinical trials indicate that an adequate number of stem cells are essential for ischemia-related organ dysfunction to be treated successfully [ 6 , 11 – 13 ]. Stem cell culture and expansion are therefore necessary [ 14 – 16 ], but these take too long for AMI patients for whom rapid treatment is critical. Accordingly, an innovative method that could provide adequate stem cells quickly without cell culture and be effective as a treatment for AMI would be of great clinical importance.…”

Cell Stress Reduction by a Novel Perfusion-Culture System Using Commercial Culture Dish

“…In vitro cell culturing also allows researchers to analyze the phenomena of life in an experimental system, which is easier than analyzing cells in vivo [7]. To repeatedly obtain stable experimental results by fully exploiting these advantages, it is necessary to apply methods that effectively culture homogeneous cells with good reproducibility (high-quality cells) [8].…”