Adipose tissue engineering: The future of breast and soft tissue reconstruction following tumor resection

Patrick, C.

doi:10.1002/1098-2388(200010/11)19:3<302::aid-ssu12>3.0.co;2-s

Cited by 194 publications

(124 citation statements)

References 33 publications

Supporting

Mentioning

123

Contrasting

Unclassified

Order By: Relevance

“…Sensitivity to hypoxia varies among cells: 40% of cells cultured under hypoxia do not survive after *5 days for endothelial cells, 42 after *12 h for cardiomyocytes, 43 and after only *2 h for preadipocytes 44 (cells of interest in adipose tissue engineering). To sustain viable (not necessarily functional) cells inside the scaffolds, O 2 transport rate must match rate of O 2 consumption, which is of the order of 1-10 nmol O 2 min À1 (10 6 cells) À1 for a range of cell types.…”

Section: Discussionmentioning

confidence: 99%

Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Buijs

Jorgensen

et al. 2009

Tissue Engineering Part A

View full text Add to dashboard Cite

The objective of this study was to investigate the influence of pore geometry on the transport rate and depth after repetitive mechanical deformation of porous scaffolds for tissue engineering applications. Flexible cubic imaging phantoms with pores in the shape of a circular cylinder, elliptic cylinder, and spheroid were fabricated from a biodegradable polymer blend using a combined 3D printing and injection molding technique. The specimens were immersed in fluid and loaded with a solution of a radiopaque solute. The solute distribution was quantified by recording 20 mm pixel-resolution images in an X-ray microimaging scanner at selected time points after intervals of dynamic straining with a mean strain of 8.6 AE 1.6% at 1.0 Hz. The results show that application of cyclic strain significantly increases the rate and depth of solute transport, as compared to diffusive transport alone, for all pore shapes. In addition, pore shape, pore size, and the orientation of the pore cross-sectional asymmetry with respect to the direction of strain greatly influence solute transport. Thus, pore geometry can be tailored to increase transport rates and depths in cyclically deformed scaffolds, which is of utmost importance when thick, metabolically functional tissues are to be engineered.

show abstract

Section: Discussionmentioning

confidence: 99%

Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Buijs

Jorgensen

et al. 2009

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…For example adipose tissue has been used in plastic surgery for a long term in order to correct body defects related to age, traumas or pathological conditions that alter patient appearance (i.e. wrinkle-filling or scar removing) (Ellenborgen 2000;Patrick 2000Patrick , 2001De Ugarte et al 2003;Casteilla et al 2004;Coleman 2004;Barrilleux et al 2006). During the last decades many authors have confirmed the advantages of lipoaspirate transplant: in fact they have noticed consistent improvement of both skin structure and tissue turgidity near treated zones (Von Heimburg et al 2001;Andrè 2002;Matsumoto et al 2006).…”

Section: Adipose Stromal Vascular Fraction Encapsulation For Cartilagmentioning

confidence: 99%

Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine

et al. 2008

View full text Add to dashboard Cite

Cell encapsulation, a strategy whereby a pool of live cells is entrapped within a semipermeable membrane, represents an evolving branch of biotechnology and regenerative medicine. For example, over the last 20 years, male and female gametes and embryos have been encapsulated with or without somatic cells for different purposes, such as in vitro gametogenesis, embryo culture, cell preservation and semen controlled release. Beside that, cell encapsulation technology in alginate, which is a natural biodegradable polymer that mimics the extracellular matrix and supports both cell functions and metabolism, has been developed with the aim of obtaining three-dimensional (3D) cultures. In this context, adipose-derived stromal vascular fraction (SVF) has attracted more and more attention because of its enormous potential in tissue regeneration. In fact, the SVF represents a rich source of mesenchymal cells (ADSCs), potentially able to differentiate into adipocytes, chondrocytes, osteoblasts, myocytes, cardiomyocytes, hepatocytes, and neuronal, epithelial and endothelial cells. These cells are ideal candidates for use in regenerative medicine, tissue engineering, including gene therapy and cell replacement cancer therapies. As long as technological resources are available for large-scale cell encapsulation intended for advanced therapies (gene therapy, somatic cell therapy and tissue engineering), the state-of-the-art in this field is reviewed in terms of scientific literature.

show abstract

“…Preadipocytes are fibroblast-like cells that uptake lipid during differentiation (Patrick, 2000;Patrick et al, in press). They grow easily with standard cell culture technologies, can be expanded ex vivo, and the molecular biology involved in preadipocyte differentiation has largely been elucidated through active research in the obesity and diabetes regimens (MacDougald and Lane, 1995;Loftus and Lane, 1997;Mandrup and Lane, 1997).…”

Section: Fat Cellsmentioning

confidence: 99%

“…The scope of this review will be limited to adipose tissue engineering applied to soft tissue defects. Adipose tissue engineering applied to breast reconstruction has been covered recently by this author (Patrick, 2000;Robb et al, in press). The three fundamental components of a tissue construct, namely cells, scaffold, and microenvironment, are discussed first, followed by proposed strategies for tissue augmentation.…”

mentioning

confidence: 99%

Tissue engineering strategies for adipose tissue repair

Patrick¹

2001

Anat. Rec.

232

160

View full text Add to dashboard Cite

Tissue engineering is a relatively young field that combines engineering, clinical science, and life sciences to, in part, repair or regrow tissues. Adipose tissue has recently become a focus area for tissue engineering, encouraged by the large number of reconstructive, cosmetic, and correctional indications that could be addressed with clinically translatable adipose tissue engineering strategies. This review discusses the three aspects of an adipose construct, namely cell types, scaffold, and microenvironment, and presents current tissue engineering strategies under pursuit.

show abstract

Adipose tissue engineering: The future of breast and soft tissue reconstruction following tumor resection

Cited by 194 publications

References 33 publications

Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Solute Transport in Cyclically Deformed Porous Tissue Scaffolds with Controlled Pore Cross-Sectional Geometries

Alginate cell encapsulation: new advances in reproduction and cartilage regenerative medicine

Tissue engineering strategies for adipose tissue repair

Contact Info

Product

Resources

About