Adjuvant Effect of Chicken Interferon-.GAMMA. for Inactivated Salmonella Enteritidis Antigen

Abstract

ABSTRACT. The adjuvant effect of chicken interferon-γ (ChIFN-γ) was examined for protecting chickens against intestinal colonization of Salmonella Enteritidis (SE) following oral exposure. Ten 7-week-old chickens per group were immunized with inactivated SE twice with or without co-administration of ChIFN-γ intramuscularly, and all chickens were challenged with SE. Sera collected from immunized groups with or without ChIFN-γ, and from unimmunized group were measured for SE antibody by agglutination test. The levels of antibodies were raised by 1 week post-immunization and did not show any difference between groups with and without ChIFN-γ. No antibodies were detected in unimmunized group before challenge. Fecal samples from each group were cultured at 1, 4, 7, and 13 days postchallenge to determine the incidence of intestinal colonization and the numbers of SE shed into the environment. Co-administration of ChIFN-γ, significantly reduced the incidence of intestinal colonization (P<0.05). At 13 days post-challenge, the bacterial counts of SE in organs were also reduced in ChIFN-γ administered group. These data suggest co-administration of ChIFN-γ with SE antigen enhances protection against SE challenge without acceleration of antibody production.

Development of a subunit vaccine containing recombinant chicken anemia virus VP1 and pigeon IFN-γ

“…It is widely accepted that neutralizing antibodies could possibly represent a valuable parameter by which to evaluate the efficacy of a vaccine against CAV (Sawant et al, 2015;Trinh et al, 2015b). Induction of cellular immune responses of IFN-␥ has been reported for a variety of antigens in previous studies (Haq et al, 2011;Takehara et al, 2003;van Slooten et al, 2000). Likewise, cell-mediated immunity, particularly the level of virusspecific IFN-␥, has been another potential correlate of protective immunity (Markowski-Grimsrud and Schat, 2003;Moeini et al, 2011;Sawant et al, 2015).…”

Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens

“…The co-administration of ChIFN-γ expressed using a baculovirus system with the inactivated Salmonella enteritidis antigen enhanced the level of protection against Salmonella enteritidis challenge without increasing the antibody production [39]. The co-administration of the recombinant ChIFN-γ with sheep red blood cell antigens resulted in stronger antibody responses and allowed a lower dose of the antigen to be used more effectively than in the chickens that received the antigen alone [25].…”

The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis

“…Cytokines are natural modulators of immune response and are being used as vaccine adjuvant to enhance host immune responses induced by DNA vaccines in coccidiosis (Kim et al 1999;Song et al 2000;Xu et al 2006). Takehara et al (2003) stated that interferon gamma (chIFN-γ) is a strong adjuvant candidate for both the general enhancement of the immune response induced by vaccine antigen and modulation of immune response generated. It induces a microbicidal state, which is involved in the resolution of the infection by intracellular parasites, thus enhancing vaccine efficacy.…”