Adjuvant effects of crystal proteins from a Mexican strain of Bacillus thuringiensis on the mouse humoral response

Calderón, Máximo E. Román; González, Juan Manuel Alcocer; Franco‐Molina, Moises Armides; Guerra, Reyes Silvestre Tamez; Padilla, Cristina Rodríguez

doi:10.1016/j.biologicals.2007.01.006

Cited by 10 publications

(4 citation statements)

References 21 publications

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“…The adjuvanticity of Cry1Ac protoxin was tested by exposing mice to microgram amount of crystallized or soluble form of the protein either intragastrically (ig), intranasally (in) or intraperitoneally (ip) 194,210,213,215,220,223,225,234,235,237,238 . Several studies demonstrated that Cry1Ac is a mucosal and systemic adjuvant as potent as other known toxins, such cholera toxin 238 , and it enhances mostly serum and intestinal IgG antibody responses, especially at the large intestine.…”

Section: Adjuvanticity and Immunogenicity Of Cry Proteinsmentioning

confidence: 99%

Literature review in support of adjuvanticity/immunogenicity assessment of proteins

Parenti

Santoro

Río

et al. 2019

EFSA Supporting Publications

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Based on the risk assessment of genetically modified plants, according to Implementing Regulation (EU) No 503/201321 "In cases when known functional aspects of the newly expressed protein or structural similarity to known strong adjuvants may indicate possible adjuvant activity, the applicant shall assess the possible role of these proteins as adjuvants". To further investigate the topic, an EFSA procurement was launched requesting a comprehensive literature review and critically appraisal on adjuvanticity and immunogenicity of proteins. A systematic literature search and critical review was performed, identifying 299 relevant publications. From the evaluation of the relevant literature emerged that: i) a clear classification of adjuvant and immunogens of proteins cannot be done; ii) structural features able to modulate adjuvanticity and immunogenicity are mainly ascribed to therapeutic proteins and in the context of allergenicity and cross-reactivity; iii) factors affecting the propensity of a protein to stimulate immune response are aggregation, thermal processing, digestion, food matrix, among others; iv) different proteins are described to have immunomodulatory effects; v) risk assessment of adjuvant and immunogenic behaviour of proteins requires specific methodologies that can be adapted from other fields; vi) adjuvanticity and immunogenicity of Cry proteins in certain experimental conditions seems plausible but due to low dosage, oral route of administration, food and feed processing and digestion, it is unlikely to emerge as a safety issue in food and feed; vii) eliciting an immune response is a very complex matter as the body responds to immune offence by inducing many processes. Based on these considerations, it is expected that the availability of new humanized animal models and the possibility to deploy artificial intelligent systems on the vastity of human data will become a general direction aiming to help answering specific questions relating to the immune systems, including the adjuvanticity and immunogenicity of food/feed proteins.

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Section: Adjuvanticity and Immunogenicity Of Cry Proteinsmentioning

confidence: 99%

Literature review in support of adjuvanticity/immunogenicity assessment of proteins

Parenti

Santoro

Río

et al. 2019

EFSA Supporting Publications

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“…These toxins are also highly specific and completely biodegradable, so no toxic products are accumulated in the environment. In fact, Calderón et al (2007) suggest the potential use of some crystal proteins as adjuvants for the administration of heterologous antigens.…”

Section: Introductionmentioning

confidence: 98%

Insecticidal crystal proteins from native Bacillus thuringiensis: numerical analysis and biological activity against Spodoptera frugiperda

et al. 2008

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Fourteen strains of Bacillus thuringiensis collected from both larvae showing disease symptoms and soil samples in northwest Argentina were characterized by insecticidal activity against Spodoptera frugiperda. First instar larvae and protein profile SDS-PAGE analysis of whole cell proteins not only allowed the differentiation of native Bacillus thuringiensis but also revealed the possibility of applying protein profile analysis in classification of toxicity patterns. Cluster analysis showed that there were two main groups. Interestingly, one of them only contained the most pathogenic native strains. The biomass-bound protease activity of native pathogenic isolates and the reference strain Bt 4D1 is also reported.

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“…These toxins are also highly specific and completely biodegradable, hence no toxic products are accumulated in the environment. In fact, Calderón et al [4] suggest the potential use of some crystal proteins as adjuvants for the administration of heterologous antigens.The activity spectrum of Bt toxins continually increases as result of the ongoing isolation of new strains around the world.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

Álvarez¹,

Loto²

2012

Biodiversity Enrichment in a Diverse World

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Adjuvant effects of crystal proteins from a Mexican strain of Bacillus thuringiensis on the mouse humoral response

Cited by 10 publications

References 21 publications

Literature review in support of adjuvanticity/immunogenicity assessment of proteins

Literature review in support of adjuvanticity/immunogenicity assessment of proteins

Insecticidal crystal proteins from native Bacillus thuringiensis: numerical analysis and biological activity against Spodoptera frugiperda

Characterization and Biological Activity of Bacillus thuringiensis Isolates that Are Potentially Useful in Insect Pest Control

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