Adjuvant treatment with Xiaoqinglong formula for bronchial asthma

Wang, Long; Zheng, Xiuli; Yi, Hui; Wang, Baojia; Yang, Yu; Feng, Xianrong; Wang, Ziyang; Ma, Li; Zhang, Xiaobo

doi:10.1097/md.0000000000017053

Cited by 9 publications

(8 citation statements)

References 7 publications

(5 reference statements)

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“…In recent years, with the aggravation of environmental problems, its morbidity and mortality rates are gradually increasing, bringing a huge burden to public health security. 16 As previously reported, patients with asthma showed lower FEV1 and higher IgE levels during the acute episode. 17,18 In the current study, 70 asthma children and 66 healthy controls were recruited.…”

Section: Discussionsupporting

confidence: 54%

Differential Expression of lncRNA CASC2 in the Serum of Childhood Asthma and Its Role in Airway Smooth Muscle Cells Proliferation and Migration

Yang¹,

Sun²,

Ren³

et al. 2022

JAA

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Asthma is a common chronic disease in children. Abnormal expression of lncRNAs can be used as biomarkers for early diagnosis of asthma. The present study aimed to explore the expression change and clinical value of lncRNA CASC2 in asthma, and further investigate its potential mechanism. Patients and Methods: Seventy asthma children and 66 healthy controls were recruited. Levels of mRNAs were detected using qRT-PCR. Receiver operating characteristic (ROC) curves were drawn for diagnostic value evaluation. Asthma cell models were established using PDGF-BB in Human airway smooth muscle cells (ASMCs). Levels of Th1/Th2 related cytokines were detected using ELISA. Lipofectamine 3000 was used for cell transfection. The target relationship was verified using luciferase activity assay. Results: CASC2 was at a low level in asthma children in comparison with the healthy controls. Serum CASC2 can distinguish healthy individuals from asthma children. Overexpression of CASC2 inhibited PDGF-BB induced cell proliferation and migration. CASC2 upregulation inhibited the release of Th2 related cytokines (IL-4 and IL-10), but promoted the release of Th1-related cytokine (IFN-γ). In PDGF-BB treated ASMCs, the reduced expression of contractile phenotype marker (α-SMA) was detected, but the trend was reversed by CASC2 upregulation. LncRNA CASC2 serves as a ceRNA of miR-31-5p, overexpression of miR-31-5p reversed the influence of CASC2 on asthma in vitro. Conclusion: Serum CASC2 can distinguish healthy individuals from asthma children. CASC2 may be involved in childhood asthma through inhibiting ASMCs proliferation, migration and inflammation via sponging miR-31-5p.

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Section: Discussionsupporting

confidence: 54%

Differential Expression of lncRNA CASC2 in the Serum of Childhood Asthma and Its Role in Airway Smooth Muscle Cells Proliferation and Migration

Yang¹,

Sun²,

Ren³

et al. 2022

JAA

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“…Therefore, it is necessary to develop new and significant treatments for asthma. Clinical studies have found that traditional Chinese medicine (TCM), a type of alternative medicine, has good clinical effects on asthma [ 6 – 8 ]. As a supplementary medicine, TCM is widely used in the treatment of asthma with good results and few side effects [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

A Network Pharmacology Approach to Reveal the Underlying Mechanisms of Rhizoma Dioscoreae Nipponicae in the Treatment of Asthma

Wang

Zhou

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. In this study, network pharmacological methods were used to analyze the targets of Rhizoma Dioscoreae Nipponicae (RDN) and investigate the potential underlying mechanism of RDN in the treatment of asthma. Methods. Asthma-related targets were obtained from the GeneCards and DisGeNET databases. The bioactive components of RDN were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database, and the targets of these compounds were predicted using the BATMAN-TCM database. The network of RDN component targets was constructed using Cytoscape. A protein-protein interaction (PPI) network was constructed in Cytoscape to determine the potential targets of RDN for the treatment of asthma. The hub genes of RDN in the treatment of asthma were screened using network topological parameters. Gene ontology (GO) and the KEGG pathways were analyzed. Molecular docking and in vivo experiments were performed to validate the network pharmacology results. Results. A total of four bioactive components and 55 targets were identified. The results of the enrichment analysis suggested that the treatment of asthma with RDN involved signaling pathways, such as those related to systemic lupus erythematosus, alcoholism, viral carcinogenesis, the cell cycle, prostate cancer, transcriptional misregulation in cancer, hepatitis B, thyroid hormone signaling, and PI3K-AKT signaling, as well as other signaling pathways. Molecular docking showed that the active components of RDN could stably bind to the predicted target. In vivo experiments showed that RDN could regulate the expression of target genes and inhibit the activation of the PI3K-AKT signaling pathway. Conclusion. To a certain extent, this study reveals the potential bioactive components and molecular mechanisms of RDN in the treatment of asthma and provides new insights for the development of new drugs for asthma.

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“…TCM has been widely used and demonstrated to be effective in the treatment of bronchial asthma (8)(9)(10). Zhike pingchuan granules (ZKPC) play a role in relieving coughing and reducing Zhike pingchuan granules improve bronchial asthma by regulating the IL-6/JAK2/STAT3 pathway YUMEI REN 1* , YULING LIU 2* , SHOUCHUAN WANG 3,4 , ZHEN LEI 5 , YONGBIN YAN 6 , XUTAO GUAN 7 , JIANGHONG HOU 8 , SHAN ZHU 9 , HAIJUN SHAN 9 , XINLEI TIAN 9 , QUAN WANG 10 , CAIHONG CAO 9 , YINGYING ZHANG 9 and YUNFENG MA 11 1 (11) demonstrated that Ginkgo lactone showed anti-inflammatory and anti-oxidant effects in a rat model of Aβ 1-40 induced Alzheimer's disease, improving nerve injury and cognitive function. Thorpe et al (12) indicated that the Gingko biloba extract EGb 761 could inhibit inflammation and thermal hyperalgesia for the treatment of inflammatory pain.…”

Section: Introductionmentioning

confidence: 99%

Zhike pingchuan granules improve bronchial asthma by regulating the IL‑6/JAK2/STAT3 pathway

Ren

Liu²,

Shouchuan

et al. 2021

Exp Ther Med

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The present study aimed to investigate the effects of zhike pingchuan granules (ZKPC) on bronchial asthma and the underlying mechanism. A bronchial asthma mouse model was established by aerosol inhalation of ovalbumin. The changes in lung pathomorphology were observed by hematoxylin and eosin staining. The levels of IL-1β, TNF-α and IL-6 in bronchoalveolar lavage fluid (BALF) and serum were detected by corresponding ELISA kits. Levels of reactive oxygen species, malondialdehyde and superoxide dismutase in lung tissues were analyzed using corresponding kits. The expression of proteins related to apoptosis and the IL-6/janus kinase 2 (JAK2)/STAT3 pathway was detected by western blot analysis. The results showed that ZKPC significantly restored the dry/wet ratio and alleviated lung pathomorphology of bronchial asthmatic mice. In addition, ZKPC inhibits inflammation, oxidative stress levels and cell apoptosis in bronchial asthmatic mice and also suppressed the IL-6/JAK2/STAT3 pathway. Fedratinib (a JAK2 inhibitor) further strengthened the alleviative effects of ZKPC on bronchial asthma. In conclusion, ZKPC improved bronchial asthma by suppressing the IL-6/JAK2/STAT3 pathway.

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Adjuvant treatment with Xiaoqinglong formula for bronchial asthma

Cited by 9 publications

References 7 publications

Differential Expression of lncRNA CASC2 in the Serum of Childhood Asthma and Its Role in Airway Smooth Muscle Cells Proliferation and Migration

Differential Expression of lncRNA CASC2 in the Serum of Childhood Asthma and Its Role in Airway Smooth Muscle Cells Proliferation and Migration

A Network Pharmacology Approach to Reveal the Underlying Mechanisms of Rhizoma Dioscoreae Nipponicae in the Treatment of Asthma

Zhike pingchuan granules improve bronchial asthma by regulating the IL‑6/JAK2/STAT3 pathway

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