Administration of a maple syrup extract to mitigate their hepatic inflammation induced by a high-fat diet: a transcriptome analysis

Kamei, Asuka; Watanabe, Yuki; Shinozaki, Fumika; Yasuoka, Akihito; Kondo, Takashi; Ishijima, Tomoko; Toyoda, Tsudoi; Arai, Soichi; Abe, Keiko

doi:10.1080/09168451.2015.1042833

Cited by 17 publications

(24 citation statements)

References 28 publications

(28 reference statements)

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“…Because no significant fluctuations were observed among the other damage markers, the liver damage in the H-group seems to be limited in extent. This is in accordance with the fact that no significant enrichment of DEGs detected in GO terms related to liver damage, such as inflammation or fibrosis [25]. …”

Section: Discussionsupporting

confidence: 86%

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

Tanaka¹,

Yasuoka

Shimizu

et al. 2017

Genes Nutr

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BackgroundTo elucidate the effects of altered dietary carbohydrate and fat balance on liver and adipose tissue transcriptomes, 3-week-old rats were fed three kinds of diets: low-, moderate-, and high-fat diets (L, M, and H) containing a different ratio of carbohydrate-fat (C-F) (65:15, 60:20, and 35:45 in energy percent, respectively).MethodsThe rats consumed the diets for 9 weeks and were subjected to biochemical and DNA microarray analyses.ResultsThe rats in the H-group exhibited lower serum triacylglycerol (TG) levels but higher liver TG and cholesterol content than rats in the L-group. The analysis of differentially expressed genes (DEGs) between each group (L vs M, M vs H, and L vs H) in the liver revealed about 35% of L vs H DEGs that were regulated in the same way as M vs H DEGs, and most of the others were L- vs H-specific. Gene ontology analysis of these L vs H DEGs indicated that those related to fatty acid synthesis and circadian rhythm were enriched. Interestingly, about 30% of L vs M DEGs were regulated in a reverse way compared with L vs H and M vs H DEGs. These reversed liver DEGs included M-up/H-down genes (Sds for gluconeogenesis from amino acids) and M-down/H-up genes (Gpd2 for gluconeogenesis from glycerol, Agpat9 for TG synthesis, and Acot1 for beta-oxidation). We also analyzed L vs H DEGs in white (WAT) and brown (BAT) adipose tissues and found that both oxidation and synthesis of fatty acids were inhibited in these tissues.ConclusionsThese results indicate that the alteration of dietary C-F balance differentially affects the transcriptomes of metabolizing and energy-storing tissues.Electronic supplementary materialThe online version of this article (doi:10.1186/s12263-017-0558-2) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 86%

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

Tanaka¹,

Yasuoka

Shimizu

et al. 2017

Genes Nutr

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“…To date, maple-derived foods are being quantified for phenolic contents based on the widely used industrial standard, namely, gallic acid equivalents (GAEs), by the Folin-Ciocalteu assay (further discussed below). 6, 7, 11, 15, 17, 19 In addition, to date, there are no chemically characterized product-specific standard/s available for the authentication of maple products.…”

Section: Introductionmentioning

confidence: 99%

Development and UFLC-MS/MS Characterization of a Product-Specific Standard for Phenolic Quantification of Maple-Derived Foods

Liu

Seeram

2016

J. Agric. Food Chem.

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The phenolic contents of plant foods are commonly quantified by the Folin-Ciocalteu assay based on gallic acid equivalents (GAEs). However, this may lead to inaccuracies since gallic acid is not always representative of the structural heterogeneity of plant phenolics. Therefore, product-specific standards have been developed for the phenolic quantification of several foods. Currently, maple-derived foods (syrup, sugar, sap/water, and extracts) are quantified for phenolic contents based on GAEs. Since lignans are the predominant phenolics present in maple, herein, a maple phenolic lignan-enriched standard (MaPLES) was purified (by chromatography) and characterized (by UFLC-MS/MS with lignans previously isolated from maple syrup). Using MaPLES and secoisolariciresinol (a commercially available lignan), the phenolic contents of the maple-derived foods increased threefold compared to GAEs. Therefore, lignan-based standards are more appropriate for phenolic quantification of maple-derived foods vs. GAEs. Also, MaPLES can be utilized for the authentication and detection of fake label claims on maple products.

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“…Composition of the HFD was varied in carbohydrate to fat, and the other ingredients were energetically made matched to the LFD. The amount of MS‐derived materials in a 0.02% MSXH‐diet is equivalent to that of the MS or MSXH used in the previous experiments . The mice were given a specific diet and water for 24 h ad libitum.…”

Section: Methodsmentioning

confidence: 99%

“…The total RNA samples were processed for DNA microarray analysis as described previously . In brief, cDNA was synthesized from 200 ng of purified total RNA, and biotinylated aRNA was then transcribed using T7 RNA polymerase by using GeneChip® 3’ IVT Express Kit (Affymetrix, Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

“…We have studied the metabolic effects of MS in reference to the hepatic transcriptome in rats and mice. In using whole MS for study with rats or MS extract (MSXH) as the ingredients in a test diet (20% w/w MS or MSXH prepared from an equal amount of MS) for study with mice, we found that MS exerts hepatoprotective effect . It is possible that these beneficial effects of MS are caused by its phenolic compounds.…”

Section: Introductionmentioning

confidence: 94%

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Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high‐fat diet

Kamei

Watanabe

Shinozaki

et al. 2016

Molecular Nutrition Food Res

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Administration of a maple syrup extract to mitigate their hepatic inflammation induced by a high-fat diet: a transcriptome analysis

Abstract: (2015) Administration of a maple syrup extract to mitigate their hepatic inflammation induced by a high-fat diet: a transcriptome analysis,

Cited by 17 publications

References 28 publications

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

Transcriptomic responses of the liver and adipose tissues to altered carbohydrate-fat ratio in diet: an isoenergetic study in young rats

Development and UFLC-MS/MS Characterization of a Product-Specific Standard for Phenolic Quantification of Maple-Derived Foods

Quantitative deviating effects of maple syrup extract supplementation on the hepatic gene expression of mice fed a high‐fat diet

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