Administration of low-dose LH induces ovulation and prevents vascular hyperpermeability and vascular endothelial growth factor expression in superovulated rats

Gómez, Raúl; Lima, Israely; Simón, Carlos; Pellicer, A.

doi:10.1530/rep.1.00129

Cited by 22 publications

(9 citation statements)

References 28 publications

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“…The same was observed in other studies [29]. Potential explanations include the promotion of growth of larger, mature follicles only by the hCG and a decreased production of endothelial growth factor (VEGF), which tends to be secreted from the relatively smaller follicles [30]. VEGF is known as an important factor in development of OHSS.…”

Section: Discussionsupporting

confidence: 64%

Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

Gomaa

Casper

Esfandiari

et al. 2012

Reprod Biol Endocrinol

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BackgroundTo compare the outcome of IVF cycles in women receiving controlled ovarian stimulation with recFSH or recFSH plus low dose hCG.MethodsA retrospective case control study, performed at a private practice affiliated with an academic institute. Patients were infertile women who were treated with IVF/ICSI and controlled ovarian stimulation in a long GnRH agonist protocol using either low dose hCG in addition to recFSH [N = 88] or recFSH alone [N = 99]. Primary outcomes were mean FSH dose, number of mature eggs, number of fertilized eggs, and serum levels of estradiol. Secondary outcomes were endometrial thickness, cycle cancellations and pregnancy rates.ResultsA significant increase in number of mature and fertilized eggs was observed in women over 40 years of age using low dose hCG in addition to recFSH. The estradiol level was significantly higher on the day of hCG administration and the serum level of FSH on cycle day 7 and on the day of hCG administration were lower.ConclusionAddition of low dose hCG to recFSH compared with recFSH alone significantly modified cycle characteristics in patients >/= 40 years and could be of potential benefit for IVF cycles in older infertile women.

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Section: Discussionsupporting

confidence: 64%

Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

Gomaa

Casper

Esfandiari

et al. 2012

Reprod Biol Endocrinol

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“…Indeed, transgenic mice overexpressing PlGF in skin under the control of keratin-14 promoter showed a substantial increase in number, branching and size of dermal blood vessels, together with enhanced vascular leakiness [37], while inhibition of PlGF was associated with reduced plasma extravasation in pathological conditions [38]. Moreover, the gonadotropin LH, which is well-known to stimulate VEGF leading to OHSS [39], has been shown to increase the follicular fluid PlGF/sFlt-1 ratio in women undergoing ovarian stimulation [40]. Further studies are needed to evaluate the potential role of PlGF in OHSS pathogenesis and examine its utility as a predictor for early and/or late OHSS.…”

Section: Discussionmentioning

confidence: 99%

Follicular fluid placental growth factor is increased in polycystic ovarian syndrome: correlation with ovarian stimulation

Tal

Seifer

Grazi

et al. 2014

Reprod Biol Endocrinol

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BackgroundPolycystic ovarian syndrome (PCOS) is characterized by increased ovarian angiogenesis and vascularity. Accumulating evidence indicates that vascular endothelial growth factor (VEGF) is increased in PCOS and may play an important role in these vascular changes and the pathogenesis of this disease. Placental growth factor (PlGF), a VEGF family member, has not been previously characterized in PCOS women. We investigated levels and temporal expression patterns of PlGF and its soluble receptor sFlt-1 (soluble Fms-like tyrosine kinase) in serum and follicular fluid (FF) of women with PCOS during controlled ovarian stimulation.MethodsThis was a prospective cohort study of 14 PCOS women (Rotterdam criteria) and 14 matched controls undergoing controlled ovarian stimulation. Serum was collected on day 3, day of hCG and day of oocyte retrieval. FF was collected on retrieval day. PlGF, sFlt-1 and anti-mullerian hormone (AMH) protein concentrations were measured using ELISA. Since sFlt-1 binds free PlGF, preventing its signal transduction, we calculated PlGF bioavailability as PlGF/sFlt-1 ratio.ResultsSerum PlGF and sFlt-1 levels were constant throughout controlled ovarian stimulation, and no significant differences were observed in either factor in PCOS women compared with non-PCOS controls at all three measured time points. However, FF PlGF levels were increased 1.5-fold in PCOS women compared with controls (p < 0.01). Moreover, FF PlGF correlated positively with number of oocytes retrieved and the ovarian reserve marker anti-mullerian hormone (AMH) and negatively with age. In addition, FF sFlt-1 levels were decreased 1.4-fold in PCOS women compared to controls (p = 0.04). PlGF bioavailability in FF was significantly greater (2-fold) in PCOS women compared with non-PCOS controls (p < 0.01).ConclusionsThese data provide evidence that FF PlGF correlates with ovarian stimulation and that its bioavailability is increased in women with PCOS undergoing controlled ovarian stimulation. This suggests that PlGF may play a role in PCOS pathogenesis and its angiogenic dysregulation.

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“…E xcept during this periovulatory period, the plasma concentrations of both FSH and LH remain at low basal levels [17,36,37]. In many mammalian species, including rats, the midcycle surge of LH drives a series of events that include oocyte maturation, follicular rupture, and luteinization of granulosa and thecal cells [15,38,39]. The LH surge is accompanied by an FSH surge that might be relevant to these processes [40].…”

Section: Discussionmentioning

confidence: 99%

“…Four rat models, the postnatal, immature, adult and acyclic aged persistent estrous (PE) rat models, are widely used in the literature [9][10][11][12][13][14][15][16][17][18][19][20][21]. Since follicular development in the rat occurs postnatally [11][12][13], we examined the postnatal rat model to determine the relationships between ovarian cAMP l e v e l , c A M P -P D E a c t i v i t y a n d f o l l i c u l a r development.…”

mentioning

confidence: 99%

“…Since follicular development in the rat occurs postnatally [11][12][13], we examined the postnatal rat model to determine the relationships between ovarian cAMP l e v e l , c A M P -P D E a c t i v i t y a n d f o l l i c u l a r development. The immature rats primed with gonadotropin model offers the synchronous nature o f f o l l i c u l a r d e v e l o p m e n t , o v u l a t i o n a n d luteinization [14][15][16]. Adult rats usually display typical estrous cycle patterns [17,18].…”

mentioning

confidence: 99%

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Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

Wang

Wei

Jiang

et al. 2007

Journal of Reproduction and Development

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Abstract. Cyclic AMP (cAMP) is a second messenger that plays a critical role in follicular recruitment, development and luteinization in the mammalian ovary. The cellular level of cAMP is largely dependent on the activity of phosphodiesterase (PDE), which degrades cAMP into 5'-AMP. The present study was conducted to investigate the level of cAMP and the activity of cAMP-PDE in postnatal rats; immature rats during gonadotropin-primed follicular development, ovulation and luteinization; adult rats during normal estrous cycling; and aged rats that spontaneously developed persistent estrous (PE) by radioimmunoassay (RIA). All four rat models were confirmed by histological examination of one ovary and assayed using the other ovary by RIA. In the postnatal rats, the ovarian cAMP level was high on day 10 after birth, while ovarian cAMP-PDE activity was highest at 21 days of age. In the immature female rats, both the ovarian cAMP level and cAMP-PDE activity increased remarkably after treatment with equine chorionic gonadotropin (eCG), increased continuously 24 h after injection of human chorionic gonadotropin (hCG) for induction of ovulation and luteinization, and then declined significantly. In the adult rats during the normal estrous cycle, the ovarian cAMP levels were low on the day of estrus, and there were no significant changes in ovarian cAMP-PDE activity throughout the estrous cycle. In the PE rats, the ovarian cAMP levels were similar to those of the adult rats on the day of estrus but were lower than those on the other days of the estrous cycle; ovarian cAMP-PDE activity was lower than that in the adult rats on any day of the estrous cycle. Together, these findings indicate that the ovarian cAMP level and cAMP-PDE activity were regulated in a stage-dependent manner during ovarian follicular development, atresia and luteinization and providing evidences that cAMP and cAMP-specific PDEs are involved in these physiological processes.

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Administration of low-dose LH induces ovulation and prevents vascular hyperpermeability and vascular endothelial growth factor expression in superovulated rats

Cited by 22 publications

References 28 publications

Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

Addition of low dose hCG to rFSh benefits older women during ovarian stimulation for IVF

Follicular fluid placental growth factor is increased in polycystic ovarian syndrome: correlation with ovarian stimulation

Changes of Cyclic AMP Levels and Phosphodiesterase Activities in the Rat Ovary

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