Adolescent intermittent ethanol exposure enhances adult stress effects in male rats

Healey, Kati L.; Kibble, Sandra; Dubester, Kira; Bell, Amelia; Swartzwelder, H. Scott

doi:10.1016/j.pbb.2022.173513

Cited by 6 publications

(3 citation statements)

References 88 publications

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“…However, results from the forced swim stress test showed a significant interaction of AIE and sex, indicating altered immobility among males and suggesting that AIE exposure may lead to differential stress responsiveness between the sexes in adulthood. This interpretation is consistent with our recent finding that AIE elevated the stress responsivity of male (but not female) rats to environmental stress (Healey et al, 2023). Thus, it may be that AIE-exposed females show an altered anxiety-like phenotype and AIE-exposed males show an altered stress-reactivity phenotype in adulthood.…”

Section: Discussionsupporting

confidence: 93%

Adolescent Intermittent Ethanol Exposure Alters Adult Exploratory and Affective Behaviors, and CerebellarGrin2BExpression in C57BL/6J Mice

Healey

Waters

Knight

et al. 2023

Preprint

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More than 90% of alcohol consumption by young people is in the pattern binge drinking. During adolescence, the brain undergoes maturational changes that influence alterations in behavior control, affective behaviors, and cerebellar brain volume and function in adulthood. We investigated long-term impacts of adolescent binge alcohol exposure on affective behaviors and cerebellar gene expression in male and female mice. We exposed C57BL/6J mice to adolescent intermittent ethanol (AIE) or air (control) vapor inhalation from postnatal day 28-42. After prolonged abstinence, in young adulthood we assessed behavior in the open field, light/dark, tail suspension, and forced swim stress tests to determine changes in affective behaviors including anxiety-like, depressive-like, and stress reactivity behavior. mRNA levels of FMR1 and other interacting gene expressions (Grin2a, Grin2B, Grm5, PSD-95, and Eaat1) were measured in the cerebellum of control and adult AIE-exposed mice. In adult AIE-exposed mice, we show decreased movement in the open field in both sexes and modest changes in females in anxiety-like behavior (center zone activity). In the forced swim stress test, adult AIE-exposed male mice spent less time immobile compared to their same-sex controls, indicative of sex-specific changes in stress reactivity. Male and female AIE-exposed mice showed increased Grin2B mRNA expression in the adult cerebellum compared to their same-sex controls. Together, these data show that adolescent binge-like ethanol exposure altered affective behaviors and modified Grin2B expression in adulthood. This indicates the cerebellum may serve as an important brain region that is susceptible to long-term molecular changes with adolescent alcohol exposure.

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Section: Discussionsupporting

confidence: 93%

Adolescent Intermittent Ethanol Exposure Alters Adult Exploratory and Affective Behaviors, and CerebellarGrin2BExpression in C57BL/6J Mice

Healey

Waters

Knight

et al. 2023

Preprint

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“…Given the high concentration in plasma, all samples were diluted 1:25 times. Then, all steps reported by the protocol were followed as reported in [ 38 ]. The microplate reader read the optical density at 405 nm, with correction between 570 and 590 nm.…”

Section: Methodsmentioning

confidence: 99%

Neuroactive Steroid–Gut Microbiota Interaction in T2DM Diabetic Encephalopathy

Diviccaro,

Cioffi,

Piazza

et al. 2023

Biomolecules

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The pathological consequences of type 2 diabetes mellitus (T2DM) also involve the central nervous system; indeed, T2DM patients suffer from learning and memory disabilities with a higher risk of developing dementia. Although several factors have been proposed as possible contributors, how neuroactive steroids and the gut microbiome impact brain pathophysiology in T2DM remain unexplored. On this basis, in male Zucker diabetic fatty (ZDF) rats, we studied whether T2DM alters memory abilities using the novel object recognition test, neuroactive steroid levels by liquid chromatography–tandem mass spectrometry, hippocampal parameters using molecular assessments, and gut microbiome composition using 16S next-generation sequencing. Results obtained reveal that T2DM worsens memory abilities and that these are correlated with increased levels of corticosterone in plasma and with a decrease in allopregnanolone in the hippocampus, where neuroinflammation, oxidative stress, and mitochondrial dysfunction were reported. Interestingly, our analysis highlighted a small group of taxa strictly related to both memory impairment and neuroactive steroid levels. Overall, the data underline an interesting role for allopregnanolone and microbiota that may represent candidates for the development of therapeutic strategies.

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“…The elevated-plus-maze (EPM) test was conducted in accordance with established procedures [31]. The EPM apparatus was elevated 60 cm from the floor, with two open arms (30 cm × 5 cm × 0.5 cm) and two closed arms (30 cm × 5 cm × 16 cm) connected by a central platform (5 cm × 5 cm).…”

Section: Elevated-plus-mazementioning

confidence: 99%

Sargassum horneri Extract Attenuates Depressive-like Behaviors in Mice Treated with Stress Hormone

Park,

Kim,

Kim

et al. 2023

Antioxidants

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Sargassum horneri, a brown seaweed, is known for its various health benefits; however, there are no reports on its effects on depression. This study aimed to investigate the antidepressant effects of S. horneri ethanol extract (SHE) in mice injected with corticosterone (CORT) and to elucidate the underlying molecular mechanisms. Behavioral tests were conducted, and corticotropin-releasing hormone (CRH), adrenocorticotropic hormone (ACTH), and CORT levels were measured. A fluorometric monoamine oxidase (MAO) enzyme inhibition assay was performed. Neurotransmitters like serotonin, dopamine, and norepinephrine levels were determined. Moreover, the ERK-CREB-BDNF signaling pathway in the prefrontal cortex and hippocampus was evaluated. Behavioral tests revealed that SHE has antidepressant effects by reducing immobility time and increasing time spent in open arms. Serum CRH, ACTH, and CORT levels decreased in the mice treated with SHE, as did the glucocorticoid-receptor expression in their brain tissues. SHE inhibited MAO-A and MAO-B activities. In addition, SHE increased levels of neurotransmitters. Furthermore, SHE activated the ERK-CREB-BDNF pathway in the prefrontal cortex and hippocampus. These findings suggest that SHE has antidepressant effects in CORT-injected mice, via the regulation of the hypothalamic-pituitary-adrenal axis and monoaminergic pathway, and through activation of the ERK-CREB-BDNF signaling pathway. Thus, our study suggests that SHE may act as a natural antidepressant.

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Adolescent intermittent ethanol exposure enhances adult stress effects in male rats

Cited by 6 publications

References 88 publications

Adolescent Intermittent Ethanol Exposure Alters Adult Exploratory and Affective Behaviors, and CerebellarGrin2BExpression in C57BL/6J Mice

Adolescent Intermittent Ethanol Exposure Alters Adult Exploratory and Affective Behaviors, and CerebellarGrin2BExpression in C57BL/6J Mice

Neuroactive Steroid–Gut Microbiota Interaction in T2DM Diabetic Encephalopathy

Sargassum horneri Extract Attenuates Depressive-like Behaviors in Mice Treated with Stress Hormone

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