ADP Requirement for prevention by a cytosolic factor of Mg2+ and Ca2+ release from rat liver mitochondria

Respiration‐dependent stimulation by inorganic phosphate of Mg2+ release from rat liver mitochondria

“…The sequence of events excludes the possibility that swelling of mitochondria is responsible for the stimulation by phosphate of Mg2' release. In accordance with the findings of Binet and Volfin [4,7] we might conclude from these data that loss of a distinct proportion of mitochondrial Mg2+ causes structural changes. Apparently that small portion of Mg2', which is delivered in the absence of phosphate or under conditions of inhibited respiration, comes from the intermembrane space and has no bearing on the permeability of the mitochondria.…”

“…The slow Mg2+ release is accelerated by Ca2+ [ 1 ] or ADP and an uncoupler [2] . Stimulation by Ca2+ of Mg2+ outflow depends on the oxidation of endogenous substrates [3]' and is prevented by ADP and the cytosolic metabolic factor of Kun and his coworkers [ 1, 2,4] .…”

Stimulation of mitochondrial calcium ion efflux by thiol-specific reagents and by thyroxine. The relationship to adenosine diphosphate retention and to mitochondrial permeability

“…The latter authors used conditions under which ADP was required as a cofactor to the cytoplasmic factor. Although ATP has a protective action, the latter authors (Binet & Volfin, 1974), and Leblanc & Clauser (1974a) agree in the finding that ADP is the more effective nucleotide. The damage resulting from exposure to high Ca2+ appears to ensue as the Ca2+ emerges after its transient accumulation; this can be inferred from the respiration-rate traces of Drahota et al (1965).…”

“…Vol. 182 al., 1971;Binet & Volfin, 1974). The latter authors used conditions under which ADP was required as a cofactor to the cytoplasmic factor.…”

Development and Use of Chlorotetracycline Fluorescence as a Measurement Assay of Chloroplast Envelope-Bound Mg2+

“…External Mg2+ interaction with the cell membrane has been associated with the maintenance of normal ion transport and electrical activity in the heart muscle (15). Membrane-associated Mg2e has been found to have a regulatory role on liver mitochondrial membrane integrity (2). This last regulatory function may involve divalent cation regulation of a K+/H+ exchange mechanism in the mitochondrial membrane (13).…”