ADPRT Val762Ala and XRCC1 Arg194Trp Polymorphisms and Risk of Gastric Cancer in Sichuan of China

Polymorphisms of XRCC1 and ADPRT Genes and Risk of Noncardia Gastric Cancer in a Chinese Population: a Case-control Study

“…These distinct patterns suggest that etiologic factors for noncardia GC may differ from those for cardia GC. In a recent study, we noted that XRCC1 Arg194Trp was associated with GC risk in Sichuan, a western province in China (Wen et al, 2012). This is consistent with findings in two other studies for overall GC risk in Southwestern and Southeastern China (Shen et al, 2009;Yuan et al, 2010).…”

“…Overall, borderline risk (Ratnasinghe et al, 2004;Yan et al, 2009) or even decreased risk (Shen et al, 2000) was observed for the XRCC1 Arg194Trp genotype for cardia GC risk, leading us to suspect that XRCC1 Arg194Trp genotypes may implicate varied host susceptibility to GC at different anatomical subsites (Shen et al, 2000). Based on our previous results (Loh et al, 2009;Wen et al, 2012), we conducted additional investigation into association of XRCC1 Arg194Trp and ADPRT Val762Ala polymorphisms with risk of the noncardia subtype in Western China.…”

Interleukin-4 and -8 Gene Polymorphisms and Risk of Gastric Cancer in a Population in Southwestern China

“…age, sex, education background and marital status) and putative risk factors such as smoking, alcohol consumption, and family history of cancer. Smokers, drinkers, family history of cancer, and cardia and non-cardia cancers were defined in our previous study (Wen et al, 2012). The research was approved by the ethics committees of four hospitals, and informed consent was obtained from all recruited subjects.…”

“…DNA extraction and SNP genotyping procedures were reported in prior studies Wen et al, 2012;Pan et al, 2013). Approximately 2-5ml of whole blood was collected after field survey and stored at -20 °C.…”

“…Primers for polymerase chain reaction amplification and single base extension (SBE) assays were designed using Sequenom Assay Design 3.1 software (Table 1). IL4-590 C>T and IL8-251T>A genotypes were analyzed using MALDI-TOF MS according to Justenhoven et al (Justenhoven et al, 2004), and procedures were explained in our previous study (Wen et al, 2012). The MassARRAY Analyzer Compact with ACQUAIRE Module (Sequenom) …”