Adrenergic Influence on the Permeability of Sheep Diaphragmatic Parietal Pleura

Salbutamol Modulation of Ion Transport in Sheep Parietal Pleura Is Protein Dependent

“…More specifically, adrenaline was found to increase the electrical resistance of the parietal pleura when applied apically in protein free medium, through stimulation of mainly b-adrenergic receptors as well as a-adrenergic receptors depending on the origin of the pleura (costal or diaphragmatic). 10,11,14) These findings were in accordance with in vivo experimental studies reproducing hydrothoraces. In these studies, b-adrenergic stimulation was found to either inhibit (in protein free) or accelerate (in the presence of protein) the resolution of the experimental hydrothoraces.…”

“…However, recent studies on the parietal pleura have revealed that the electrophysiological properties of the parietal pleura are influenced by adrenergic stimulation as it has been demonstrated by transmesothelial electrical resistance measurements. 10,11) Furthermore, previous in vivo studies in rabbits have shown that b 2 -adrenergic stimulation decreased the net rate liquid absorption of Ringer's lactate hydrothoraces by 25%, while b 2 -adrenergic stimulation in the setting of 1% albumin-Ringer's lactate hydrothoraces increased it by 29%. 8) These findings have been attributed to amiloride insensitive Na ϩ transport alterations.…”

“…6,7) Interestingly, it has been already shown that pleural fluid clearance is affected by b 2 -agonists, influencing solute coupled liquid absorption through the mesothelium. [8][9][10][11] In physiological conditions solute coupled liquid absorption through the mesothelium is the main pathway of pleural fluid absorption and clearance. In pleural effusions lymphatic drainage is the main route of fluid absorption, while solute coupled liquid absorption is still a key mechanism of fluid removal.…”

“…In pleural effusions lymphatic drainage is the main route of fluid absorption, while solute coupled liquid absorption is still a key mechanism of fluid removal. [8][9][10][11] The direct effect of salbutamol in the presence of protein on the parietal pleural membrane has never been studied. However, recent studies on the parietal pleura have revealed that the electrophysiological properties of the parietal pleura are influenced by adrenergic stimulation as it has been demonstrated by transmesothelial electrical resistance measurements.…”

“…8) These findings have been attributed to amiloride insensitive Na ϩ transport alterations. 8,10,11) The objective of this study was to investigate the effect of salbutamol on the parietal pleura equivalent short circuit current (I E ). Our experiments were oriented in mimicking the effect of salbutamol in conditions of exudative pleural effusions (high protein content) that are encountered in ARDS, by applying it apically on the parietal pleura.…”