ADRENO-CORTICAL AND MEDULLARY RESPONSES TO ADENOSINE 3′,5′-MONOPHOSPHATE¹

The Assay of Adenosine-3',5'-phosphate in Skeletal Muscle*

“…In other particulate and slice preparations cyclic-AMP production is stimulated by serotonin (Mansour et al, 1960), vasopressin (Brown et al, 1963), ACTH (Haynes, 1958), and thyrotropic hormone (Klainer et al, 1962). Cyclic AMP has been said to increase steroid production in adrenal slices (Hayes ef al., 1959) and perfused adrenal (Hilton et al, 1961), to promote water reabsorption in toad bladder (in a manner similar to vasopressin) (Orloff and Handler, 1962), and to stimulate relaxing-factor particles in skeletal muscle (Uchida and Mommaerts, 1963). The suggestions have been made that the inotropic effect of epinephrine on cardiac muscle is mediated by cyclic AMP through phosphorylase activation, or possibly by cyclic AMP independent of its effect on phosphorylase (Mayer et al, 1963).…”

Cleavage of Cholesterol Side Chain by Adrenal Cortex. IV. Effect of Phosphate and Various Nucleotides on a Soluble Enzyme System*

“…With intact cells, corticosteroid production is stimulated by ACTH, 3',5'-AMP (Haynes et al, 1959;Koritz, 1962b;Birmingham et al, 1960;Hilton et al, 1961), Ca2+ (Birmingham et al, 1960), TPNH, and a combination of TPN+ and G-6-P (Koritz and Perón, 1958; Koritz, 1962b). The effect of ACTH requires also glucose and Ca2+ (Schonbaum et al, 1956;Birmingham et al, 1960) and is inhibited by puromycin (Ferguson, 1963).…”

Effects of Adenosine-3', 5'-monophosphate and 5-Hydroxytryptamine on the Secretory Activity of the Canine Testis

“…Adenosine-3?, 5?-monophosphate has been shown to be effective in the steroidogenesis in the adrenal cortex (Hynes 1958;Hilton et al 1961) and testis (Eik-Nes 1967). On the other hand, the effect of 5-hydroxytryptamine on androgen production and release by the testis is poorly understood although this problem has been studied in the adrenal cortex (Rosenkrantz 1959;Verdesca et al 1961).…”