Adseverin modulates morphology and invasive function of MCF7 cells

Tanic, Jelena; Wang, Y.; Lee, W.; Coelho, Nuno M.; Glogauer, Michael; McCulloch, Christopher A.

doi:10.1016/j.bbadis.2019.07.015

Cited by 3 publications

(2 citation statements)

References 29 publications

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“…High levels of SCIN were observed in gastric cancer and silenced SCIN suppressed metastasis of gastric cancer cells, and decreased filopodium formation[ 18 ]. SCIN is involved in subcortical actin remodeling and promotes the formation of cell extensions and collagen degradation in MCF7 cells, thereby affecting matrix invasion and metastasis[ 25 ]. In this study, aggregated and arranged actin stress fibers were observed in the glioma cells, while the knockdown of SCIN caused the formation of sparse and disordered actin stress fibers.…”

Section: Discussionmentioning

confidence: 99%

Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

Lin,

Zhao,

Sun

et al. 2024

World J Clin Oncol

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BACKGROUND Glioma is one of the most common intracranial tumors, characterized by invasive growth and poor prognosis. Actin cytoskeletal rearrangement is an essential event of tumor cell migration. The actin dynamics-related protein scinderin (SCIN) has been reported to be closely related to tumor cell migration and invasion in several cancers. AIM To investigate the role and mechanism of SCIN in glioma. METHODS The expression and clinical significance of SCIN in glioma were analyzed based on public databases. SCIN expression was examined using real-time quantitative polymerase chain reaction and Western blotting. Gene silencing was performed using short hairpin RNA transfection. Cell viability, migration, and invasion were assessed using cell counting kit 8 assay, wound healing, and Matrigel invasion assays, respectively. F-actin cytoskeleton organization was assessed using F-actin staining. RESULTS SCIN expression was significantly elevated in glioma, and high levels of SCIN were associated with advanced tumor grade and wild-type isocitrate dehydrogenase. Furthermore, SCIN-deficient cells exhibited decreased proliferation, migration, and invasion in U87 and U251 cells. Moreover, knockdown of SCIN inhibited the RhoA/focal adhesion kinase (FAK) signaling to promote F-actin depolymerization in U87 and U251 cells. CONCLUSION SCIN modulates the actin cytoskeleton via activating RhoA/FAK signaling, thereby promoting the migration and invasion of glioma cells. This study identified the cancer-promoting effect of SCIN and provided a potential therapeutic target for the treatment of glioma.

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Section: Discussionmentioning

confidence: 99%

Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

Lin,

Zhao,

Sun

et al. 2024

World J Clin Oncol

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“…The actin-binding protein Adseverin, a member of the Gelsolin superfamily, has been identified as containing multiple proteins with the ability to promote F-Actin depolymerization and nucleation. Adseverin has been indicated as a mediating factor in osteoclastogenesis (Hassanpour, Jiang, Wang, Kuiper, & Glogauer, 2014), cancer cell invasion and proliferation (Jian et al, 2018;Tanic et al, 2019), and cellular exocytosis (Bruun, Høy, & Gromada, 2000;Trifar o, Gasman, & Gutiérrez, 2008). From the limited investigations into Adseverin that have been performed, the protein appears to have a role in the regulation of the stability of the actin cytoskeleton (Chellaiah et al, 2000;Cunningham, Stossel, & Kwiatkowski, 1991;Dumitrescu Pene, Rosé, Lejen, Marcu, & Trifar o, 2005).…”

Section: Introductionmentioning

confidence: 99%

The actin‐binding protein Adseverin mediates neutrophil polarization and migration

et al. 2021

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Through the process of chemotactic migration, neutrophils are able to recruit to an inflammatory site and eliminate pathogens, thus playing a vital role in host defense.The process of neutrophil chemotaxis is mediated by dynamic actin reorganization and polymerization. Adseverin, an actin-binding protein and member of the Gelsolin superfamily of proteins, has been hypothesized to regulate goal directed movement through the capping and severing of actin filaments, but has never been investigated in the context of neutrophil chemotaxis. Using an Adseverin knockout mouse model, we show that Adseverin plays a role in subcortical F-actin assembly at the leading edge during chemotaxis through the generation of free barbed ends on existing actin filaments. In addition, in the absence of Adseverin, neutrophils show reduced speed of migration in vitro and in vivo. This study indicates that Adseverin is a regulator of actin filament generation during neutrophil chemotaxis.

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The AHR target gene scinderin activates the WNT pathway by facilitating the nuclear translocation of β-catenin

Perez‐Castro

Venkateswaran

Garcia

et al. 2022

Journal of Cell Science

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The ligand-activated transcription factor Aryl Hydrocarbon Receptor (AHR) regulates cellular detoxification, proliferation, and immune evasion in a range of cell types and tissues, including cancer cells. In this study, we used RNA-seq to identify the signature of AHR target genes regulated by the pollutant 2,3,7,8-tetrachlorodibenzodioxin (TCDD) and the endogenous ligand kynurenine (Kyn), a tryptophan-derived metabolite. This approach identified a signature of six genes (CYP1A1, ALDH1A3, ABCG2, ADGFR1, and SCIN) as commonly activated by endogenous or exogenous ligands of AHR in multiple colon cancer cell lines. Among these, the actin-severing protein Scinderin (SCIN) was necessary for cell proliferation; SCIN downregulation limited cell proliferation and its expression increased it. SCIN expression is elevated in a subset of colon cancer patient samples, which also contain elevated β-catenin levels. Remarkably, SCIN expression promotes nuclear translocation of β-catenin and activates the WNT pathway. Our study identified a new mechanism for adhesion-mediated signaling in which SCIN, likely via its ability to alter the actin cytoskeleton, facilitates the nuclear translocation of β-catenin.

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Adseverin modulates morphology and invasive function of MCF7 cells

Cited by 3 publications

References 29 publications

Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

Scinderin promotes glioma cell migration and invasion via remodeling actin cytoskeleton

The actin‐binding protein Adseverin mediates neutrophil polarization and migration

The AHR target gene scinderin activates the WNT pathway by facilitating the nuclear translocation of β-catenin

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