Adsorbed and Bound Residues in Fulvic Acid Fractions of a Contaminated Groundwater - Isolation:, Chromatographic and Spectroscopic Characterization

Zwiener, Christian; Kumke, Michael U.; Abbt‐Braun, Gudrun; Frimel, F. H.

doi:10.1002/(sici)1521-401x(199907)27:4<208::aid-aheh208>3.0.co;2-v

Cited by 18 publications

(15 citation statements)

References 11 publications

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“…[35,36] For that purpose, the fulvic acid solution obtained (about 130 mL) was laid on a XAD-7 column (Merck, Darmstadt, Germany; length: 20 cm, diameter: 2 cm). The column was washed with 12 mL of water (which was discarded), and the fulvic acids were eluted successively with 20 mL of 0.1 M NaOH and 60 mL of water.…”

Section: Fractionation Of the Non-extractable Soil Residues Derived Fmentioning

confidence: 99%

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Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Junge

Meyer

Ciecielski

et al. 2011

J. of Env. Sc. & Hlth., Part B

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Recently, we reported on soil fate of SDZ residues amended with pig manure treated with ¹⁴C-labeled sulfadiazine ¹⁴C-SDZ). The first objective of the present study was to determine whether this strategy can be substituted by application of ¹⁴C-SDZ to soil. The second objective was to characterize non-extractable SDZ residues by fractionation, size exclusion chromatography (SEC) and solid state ¹³C-NMR. The fate of ¹⁴C-SDZ was examined for 28 d, using two soils with and without amendment of pig manure. Mineralization of ¹⁴C-SDZ was low; extractable residues decreased to 7-30%. Compared to the previous study, results were similar. ¹⁴C-SDZ derived bound radioactivity was found in HCl-washings, fulvic, humic acids and humin. According to SEC, one bound ¹⁴C portion (70%) co-eluted with fulvic acids (above 910 g mol⁻¹); the other consisted of adsorbed/entrapped ¹⁴C-SDZ. The ¹³C-SDZ study was performed for 30 d; humic acids were examined by ¹³C-NMR. A signal (100-150 ppm) was referred to ¹³C-SDZ. SEC and ¹³C-NMR demonstrated rapid integration of SDZ into humics.

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Section: Fractionation Of the Non-extractable Soil Residues Derived Fmentioning

confidence: 99%

“…Prior to further characterization, the fulvic acids fractions were thus, purified twice over XAD-7 columns. [35,36] The recovery of Downloaded by [Northeastern University] at 18:47 03 January 2015 Fig. 2.…”

Section: Fractionation Of the 14 C-sdz Derived Non-extractable Residuesmentioning

confidence: 99%

Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Junge

Meyer

Ciecielski

et al. 2011

J. of Env. Sc. & Hlth., Part B

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show abstract

“…[17,21,22] For that purpose, the fulvic acid solution obtained after precipitation of the humic acids (about 130 mL; see before) was laid on an XAD-7 column (length: 20 cm, diameter: 2 cm; Merck, Darmstadt, Germany). The column was washed with 12 mL of water, and fulvic acids were eluted successively with 20 mL of 0.1M NaOH and 60 mL of water.…”

Section: Extraction and Sample Preparationmentioning

confidence: 86%

Metabolic fate of the¹⁴C-labeled herbicide clodinafop-propargyl in a sediment–water system

Yuan

Weitzel

Schäffer

et al. 2015

Journal of Environmental Science and Health, Part B

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The metabolic fate of (14)C-phenyl-labeled herbicide clodinafop-propargyl ((14)C-CfP) was studied for 28 days in lab assays using a sediment-water system derived from a German location. Mineralization was 5.21% of applied (14)C after 28 days exhibiting a distinct lag phase until day 14 of incubation. Portions of radioactivity remaining in water phases decreased at moderate rate to 18.48% after 28 days; 62.46% were still detected in water after 14 days. Soxhlet extraction of the sediment using acetonitrile released 35.56% of applied (14)C with day 28, while 33.99% remained as non-extractable residues. A remarkable increase of bound (14)C was observed between 14 and 28 days correlating with the distinct increase of mineralization. No correlation was found throughout incubation with microbial activity of the sediment as determined by dimethyl sulfoxide reduction. Dissolved oxygen and pH value of water phases remained almost constant for 28 days. Analyses of Soxhlet extracts of the sediment and ethyl acetate extracts of water phases by radio-TLC and radio-HPLC revealed that CfP was rapidly cleaved to free acid clodinafop (Cf), which was further (bio-) transformed. DT50 values (based on radio-HPLC) were below 1 day (CfP) and slightly above 28 days (Cf). Further metabolites were not detected. Fractionation of humic and non-humic components of the sediment demonstrated that CfP's non-extractable residues were predominantly associated with fulvic acids up to 14 days of incubation (3.36%), whereas after 28 days, the majority of radioactivity was found in the humin/mineral fraction (13.30% of applied (14)C). Due to high-performance size-exclusion chromatography of the fulvic acids fraction derived from assays incubated for 28 days, this portion of (14)C was firmly, possibly covalently bound to fulvic acids and did not consist of CfP or Cf. Using an isolation strategy comprising preincubation of sediment with CfP and mineralization of (14)C-CfP as criterion, a microorganism was isolated from the sediment examined. It grew on (14)C-CfP as sole carbon source with evolution of (14)CO2. The bacterium was characterized by growth on commonly used carbon sources and 16S rDNA sequence analysis. Its sequence exhibited high similarity with that of Nocardioides aromaticivorans strain H-1 (98.85%; DSM 15131, JCM 11674).

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“…Similar associations and assimilations of low molecular weight compounds into aquatic geopolymers, aggregates or organo-mineral complexes were observed (e.g., Murphy et al 1990;Chin and Gschwend 1992;Buffle and Leppard 1995;Klaus et al 1998;Zwiener et al 1999). An important aspect especially in riverine systems is the mobilisation of pollutants associated with colloids, suspended particulate matter or dissolved geopolymers and the subsequent enhanced spatial distribution (s.a. McCarthy and Zachara 1989;Johnson and Amy 1995).…”

Section: Introductionmentioning

confidence: 99%

“…Non-destructive methods include IR-, UV/VIS-, NMR-and ESR-spectroscopy, also in combination with liquid chromatography or size exclusion chromatography (e.g. Burns et al 1973;Senesi et al 1987;Schlautmann and Morgan 1993;Fabbri et al 1998b;Nanny 1999;Zwiener et al 1999). Destructive analytical approaches transfer the bound or macromolecular fraction into low molecular weight compounds either by pyrolysis or by chemical degradation.…”

Section: Introductionmentioning

confidence: 99%

Organic Contaminants in Riverine and Groundwater Systems

2006

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Adsorbed and Bound Residues in Fulvic Acid Fractions of a Contaminated Groundwater - Isolation:, Chromatographic and Spectroscopic Characterization

Cited by 18 publications

References 11 publications

Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Metabolic fate of the¹⁴C-labeled herbicide clodinafop-propargyl in a sediment–water system

Organic Contaminants in Riverine and Groundwater Systems

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Adsorbed and Bound Residues in Fulvic Acid Fractions of a Contaminated Groundwater - Isolation:, Chromatographic and Spectroscopic Characterization

Cited by 18 publications

References 11 publications

Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Characterization of non-extractable 14C- and 13C-sulfadiazine residues in soil including simultaneous amendment of pig manure

Metabolic fate of the14C-labeled herbicide clodinafop-propargyl in a sediment–water system

Organic Contaminants in Riverine and Groundwater Systems

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Metabolic fate of the¹⁴C-labeled herbicide clodinafop-propargyl in a sediment–water system