Dermatophytosis is a fungal infection of the skin caused by a group of pathogenic fungi known as dermatophytes. Therefore, we investigated those medicinal plants that were being used by local people and traditional healers to treat skin infections in the Mopani District, Limpopo Province. A survey was conducted through the use of a semistructured program to gather information on the common names, plant parts used, methods of preparation, and administration of the medicine. Findings of the survey revealed that over 30 plant species were used for the treatment of skin infections in the area. The most common local mode of medicinal preparation to treat skin was decoction (37%), followed by paste (21%), infusion (19%), poultice (9%), smoke (7%), maceration (5%), and steam (2%). Of the species, only 12 ( Ficus sur L., Peltophorum africanum Sond., Vangueria infausta Burch. subsp. infausta, Diospyros mespilliformis Hochst. ex. A. DC., Ziziphus mucronata Willd. subsp. mucronata, Euclea divinorum Hiern, Ximenia caffra Sond., Dombeya rotundifolia Hochst., Ficus sycomorus L., Sideroxylon inerme L. subsp inerme, Parinari curattellifolia Planch. ex Benth., and Maytenus undata (Thunb.) Blakelock) were selected based on literature and ethnobotanical information. We further investigated the antifungal activity of acetone and aqueous extracts of the above mentioned selected plant species using serial dilution assay against Trichophyton rubrum, Microsporum canis, and Candida albicans. All plant extracts were active against the tested microorganisms with minimum inhibitory concentration (MIC) values ranging between 0.02 mg/mL and 1.25 mg/mL. In the bioautography assay, more active compounds were visible in acetone and water extracts of E. divinorum. No active compounds were observed in some plant extracts with excellent antifungal activity, as shown in the microdilution assay. Findings, in general, suggest that the identified plant species, especially those with extracts showing relatively low MIC values, are playing a big role in treating skin infections in Mopani District.
