Effect of fungal treatment on composition, tannin levels, and digestibility of black locust (Robinia pseudoacacia) leaves.

Rakesh, Dev Dutt; Bhat, T. K.; Singh, Bhupinder

doi:10.2323/jgam.46.99

Cited by 12 publications

(7 citation statements)

References 14 publications

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“…A fungus identified as Aspergillus niger van Tieghem having tannin-protein complex-degrading activity was isolated from the faeces of hill cattle by Bhat et al [26,27]. This fungus which is a prolific degrader of tannins has high tannase activity [184] and has been tried to upgrade feed value of a tannin-rich tree fodder, Robinia pseudoacacia [168]. Recently, a tannase-producing bacterial strain Enterobacter ludwigii has been isolated from the rumen of migratory goats exposed to tannin-rich browse and may be of use for reducing tannin toxicity in these animals [197].…”

Section: Solid-state Fermentationmentioning

confidence: 99%

Value Addition of Feed and Fodder by Alleviating the Antinutritional Effects of Tannins

et al. 2013

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Tannins are one of the important plant secondary metabolites having wide prevalence in the plant kingdom. They are a prominent constituent of various types of feed, fodder and agro-industrial wastes. The intake of tannins at a low level has recently been found to have some positive effects in ruminants. However, the use of tannin-rich biomass as animal feed, having high content of tannins, is limited by the antinutritional effects of tannins at this level in an animal system. A number of physical, chemical, biological and miscellaneous approaches have been developed for inactivation or removal of tannins for enhancement of the feeding value of tannin-rich biomass. However, none of the individual method is successful in total inactivation or removal of tannins without loss of nutritive value, and this limits the utilization of a vast amount of plant resource. A cohesive and an integrated detanninification strategy is required for alleviating the antinutritional effects of tannins in animals and upgrading the feeding value of tanniniferous biomass.

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Section: Solid-state Fermentationmentioning

confidence: 99%

Value Addition of Feed and Fodder by Alleviating the Antinutritional Effects of Tannins

et al. 2013

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“…The difference in digestibility in the above two treatments was reported to be due to the different nature of leaves, fungi and fermentation times [30]. Rakesh et al [65] studied the effects of fungal treatment on composition, tannin levels and digestibility of black locust leaves and reported a 30% decrease in tannins after 30 days. Singh et al [72] used an in vitro syringe system and reported the degradation of tannic acid to gallic acid, pyrogallol and resorcinol in 24, 48 and 72 h, respectively, by mixed microflora of ruminal fluid from cattle that had no prior exposure to tannin-rich forage.…”

Section: Implication Of Tanninolytic Microorganismsmentioning

confidence: 99%

Interaction of gut microflora with tannins in feeds

Goel

Puniya

Aguilar

et al. 2005

Naturwissenschaften

164

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Tannins (hydrolyzable and condensed) are water-soluble polyphenolic compounds that exert antinutritional effects on ruminants by forming complexes with dietary proteins. They limit nitrogen supply to animals, besides inhibiting the growth and activity of ruminal microflora. However, some gastrointestinal microbes are able to break tannin-protein complexes while preferentially degrading hydrolyzable tannins (HTs). Streptococcus gallolyticus, Lonepinella koalarum and Selenomonas ruminantium are the dominant bacterial species that have the ability to degrade HTs. These tanninolytic microorganisms possess tannin-degrading ability and have developed certain mechanisms to tolerate tannins in feeds. Hence, selection of efficient tanninolytic microbes and transinoculation among animals for long-term benefits become areas of intensive interest. Here, we review the effects of tannins on ruminants, the existence and significance of tannin-degrading microorganisms in diverse groups of animals and the mechanisms that tannin-degrading microorganisms have developed to counter the toxic effects of tannin.

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“…have tannin degrading activity and the enzyme tannase plays a prominent role in the degradation of gallotannins (LEKHA and LONSANE 1996). Microbial, especial fungal treatments have been tried for the reduction of tannin content and nutritive enhancement of the treated material (GAMBLE et al 1996, RAKESH et al 2000.…”

mentioning

confidence: 99%

Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425

Bhardwaj

Singh

Bhat

2003

J. Basic Microbiol.

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The present investigation was carried out for increasing the yield of tannase of Aspergillus niger and the physico-chemical characterization of this enzyme. the extraction of enzyme protein. However, extraction of fungal pigments and proteins was observed to have high pH dependence, and maximum enzyme extraction was obtained at pH 5.5. The two-step purification protocol gave 51-fold purified enzyme with a yield of 20%. The total tannase activity was made up of nearly equal activity of esterase and depsidase. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of purified tannase protein indicated it to be made up of two polypeptides of molecular weight 102 and 83 kDa. Based on the Michaelis-Menten constant (Km) of tannase for three substrates tested, tannic acid was the best substrate with Km of 2.8 x 10(-4) M, followed by methyl gallate and propyl gallate. The inhibition was maximum for CaCl2 (58%) whereas EDTA had no modulatory effect on tannase activity. The inhibitor binding constant (KI) of CaCl2 was 5.9 x 10(-4) M Homogenization and detergent pretreatments did not have any remarkable effect on and the inhibition was of noncompetitive type.

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Effect of fungal treatment on composition, tannin levels, and digestibility of black locust (Robinia pseudoacacia) leaves.

Cited by 12 publications

References 14 publications

Value Addition of Feed and Fodder by Alleviating the Antinutritional Effects of Tannins

Value Addition of Feed and Fodder by Alleviating the Antinutritional Effects of Tannins

Interaction of gut microflora with tannins in feeds

Purification and characterization of tannin acyl hydrolase from Aspergillus niger MTCC 2425

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