Adsorption and activity of Thermomyces lanuginosus lipase on hydrophobic and hydrophilic surfaces measured with dual polarization interferometry (DPI) and confocal microscopy

Sonesson, Andreas W.; Callisen, Thomas H.; Brismar, Hjalmar; Elofsson, Ulla

doi:10.1016/j.colsurfb.2007.08.005

Cited by 24 publications

(23 citation statements)

References 33 publications

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“…Interactions of lipases with a range of different interfaces including lipid bilayers have been investigated using many experimental and computational techniques [20] , [50] , [56] , [57] , [58] , [59] . However, the mechanisms of their association with lipid bilayer and related interfaces remain incompletely understood, especially in the context of lipase activation, although electrostatic interactions are generally considered important.…”

Section: Discussionmentioning

confidence: 99%

Interfacial activation of M37 lipase: A multi-scale simulation study

Willems

Lelimousin

Koldsø

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Lipases are enzymes of biotechnological importance that function at the interface formed between hydrophobic and aqueous environments. Hydrophobic interfaces can induce structural transitions in lipases that result in an increase in enzyme activity, although the detailed mechanism of this process is currently not well understood for many lipases. Here, we present a multi-scale molecular dynamics simulation study of how different interfaces affect the conformational dynamics of the psychrophilic lipase M37. Our simulations show that M37 lipase is able to interact both with anionic lipid bilayers and with triglyceride surfaces. Interfacial interactions with triglyceride surfaces promote large-scale motions of the lid region of M37, spanning residues 235–283, revealing an entry pathway to the catalytic site for substrates. Importantly, these results suggest a potential activation mechanism for M37 that deviates from other related enzymes, such as Thermomyces lanuginosus lipase. We also investigated substrate binding in M37 by using steered MD simulations, confirming the open state of this lipase. The exposure of hydrophobic residues within lid and active site flap regions (residues 94–110) during the activation process provides insights into the functional effect of hydrophobic surfaces on lipase activation.

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Section: Discussionmentioning

confidence: 99%

Interfacial activation of M37 lipase: A multi-scale simulation study

Willems

Lelimousin

Koldsø

et al. 2017

Biochimica et Biophysica Acta (BBA) - Biomembranes

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“…Adsorption can also stabilize the structure of some proteins and hence improve their resistance to denaturation as compared to dissolved proteins [ 115 ]. Also, it was shown experimentally that the activity of enzymes after surface adsorption can be indifferent [ 116 ] or reduced [ 117 ] compared to dissolve enzymes if the orientation of the active sites is directed toward the solution or toward the surface, respectively. Some proteins or peptides exhibit their function only after adsorption [ 118 ].…”

Section: Probing Transmembrane Proteins On Solid Surfacesmentioning

confidence: 99%

Engineering Lipid Bilayer Membranes for Protein Studies

Khan

Dosoky

Williams

2013

IJMS

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Lipid membranes regulate the flow of nutrients and communication signaling between cells and protect the sub-cellular structures. Recent attempts to fabricate artificial systems using nanostructures that mimic the physiological properties of natural lipid bilayer membranes (LBM) fused with transmembrane proteins have helped demonstrate the importance of temperature, pH, ionic strength, adsorption behavior, conformational reorientation and surface density in cellular membranes which all affect the incorporation of proteins on solid surfaces. Much of this work is performed on artificial templates made of polymer sponges or porous materials based on alumina, mica, and porous silicon (PSi) surfaces. For example, porous silicon materials have high biocompatibility, biodegradability, and photoluminescence, which allow them to be used both as a support structure for lipid bilayers or a template to measure the electrochemical functionality of living cells grown over the surface as in vivo. The variety of these media, coupled with the complex physiological conditions present in living systems, warrant a summary and prospectus detailing which artificial systems provide the most promise for different biological conditions. This study summarizes the use of electrochemical impedance spectroscopy (EIS) data on artificial biological membranes that are closely matched with previously published biological systems using both black lipid membrane and patch clamp techniques.

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“…Swann et al [ 81 ] proposed an elegant matrix that assists interpreting DPI data by correlating the different parameters (thickness, density, mass coverage) calculated from both the TM and TE responses. This type of detailed information can be extremely helpful for characterizing the conformational changes of macromolecular interactions [ 81 , 84 , 85 ] and the design of surfaces for optical biosensors [ 38 , 86 ].…”

Section: Dual Polarization Interferometry Biosensormentioning

confidence: 99%

Theory and Applications of Surface Plasmon Resonance, Resonant Mirror, Resonant Waveguide Grating, and Dual Polarization Interferometry Biosensors

Daghestani

Day

2010

Sensors

195

119

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Biosensors have been used extensively in the scientific community for several purposes, most notably to determine association and dissociation kinetics, protein-ligand, protein-protein, or nucleic acid hybridization interactions. A number of different types of biosensors are available in the field, each with real or perceived benefits over the others. This review discusses the basic theory and operational arrangements of four commercially available types of optical biosensors: surface plasmon resonance, resonant mirror, resonance waveguide grating, and dual polarization interferometry. The different applications these techniques offer are discussed from experiments and results reported in recently published literature. Additionally, recent advancements or modifications to the current techniques are also discussed.

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Adsorption and activity of Thermomyces lanuginosus lipase on hydrophobic and hydrophilic surfaces measured with dual polarization interferometry (DPI) and confocal microscopy

Cited by 24 publications

References 33 publications

Interfacial activation of M37 lipase: A multi-scale simulation study

Interfacial activation of M37 lipase: A multi-scale simulation study

Engineering Lipid Bilayer Membranes for Protein Studies

Theory and Applications of Surface Plasmon Resonance, Resonant Mirror, Resonant Waveguide Grating, and Dual Polarization Interferometry Biosensors

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