Adsorption of Beta-Adrenergic Agonists Used in Sport Doping on Metal Nanoparticles: A Detection Study Based on Surface-Enhanced Raman Scattering

Izquierdo-Lorenzo, Irene; Sánchez-Cortés, Santiago; Garcı́a-Ramos, José Vicente

doi:10.1021/la102590f

Cited by 75 publications

(49 citation statements)

References 33 publications

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“…It is worth mentioning that the shape of the corresponding curves allows getting insight into peptide−peptide and peptide−metal interactions, thus revealing the differences between the adsorption mechanisms corresponding to different colloid types. 28,29 As a consequence, a strong peptide−peptide interaction can be deduced in the case of SST-14 adsorbed on Ag NPs.…”

Section: ■ Results and Discussionmentioning

confidence: 98%

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles

et al. 2015

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International audienceDespite numerous investigations devoted to the control of peptide and protein self-assemblies using nanostructured materials, the molecular details of the peptide-material interaction sites remain still under debate. Here, we suggest a solution by using jointly the middle- and low wavenumber regions of surface-enhanced Raman spectra. To achieve our goal, adequately prepared gold and silver colloids, of which the nanofabrication was controlled by means of zeta potential and extinction spectra, were used to enhance the Raman signal arising from a natural fibrillogenic peptide hormone, somatostatin-14, at very low (10–6-to-10–8 M) concentrations. Transmission electron microscopy has revealed that the interacting partners are involved in a mutual aggregation process. In fact, while the presence of plasmonic colloids facilitates the aggregation of the peptide, leading to the formation of the so-called “corona” around nanoparticles, we could observe the appearance of relatively large size peptide-nanoparticle agglomerates in solution. However, the comparison between the data obtained from gold and silver nanoparticles is consistent with the fact that the nature of metal surface, as well as its ionic coverage, considerably affect the peptide binding and subsequent aggregation process. Furthermore, this work led us to conclude that the adsorption of somatostatin-14 on silver nanoparticles is rendered possible through an ionic pair interaction, whereas its anchoring on gold nanoparticles occurs by a direct binding, in which the metal atom and the nitrogen of the unique peptide tryptophan residue are involved

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Section: ■ Results and Discussionmentioning

confidence: 98%

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles

et al. 2015

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“…In addition to narcotics, doping in athletics calls for quick and simple testing. Doping drugs, such as clenbuterol, salbutamol, and terbutaline, can be detected using SERS and AuNPs [201].…”

Section: Sers Of Small Moleculesmentioning

confidence: 99%

Fundamentals and applications of SERS-based bioanalytical sensing

et al. 2017

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Abstract:Plasmonics is an emerging field that examines the interaction between light and metallic nanostructures at the metal-dielectric interface. Surface-enhanced Raman scattering (SERS) is a powerful analytical technique that uses plasmonics to obtain detailed chemical information of molecules or molecular assemblies adsorbed or attached to nanostructured metallic surfaces. For bioanalytical applications, these surfaces are engineered to optimize for high enhancement factors and molecular specificity. In this review we focus on the fabrication of SERS substrates and their use for bioanalytical applications. We review the fundamental mechanisms of SERS and parameters governing SERS enhancement. We also discuss developments in the field of novel SERS substrates. This includes the use of different materials, sizes, shapes, and architectures to achieve high sensitivity and specificity as well as tunability or flexibility. Different fundamental approaches are discussed, such as label-free and functional assays. In addition, we highlight recent relevant advances for bioanalytical SERS applied to small molecules, proteins, DNA, and biologically relevant nanoparticles. Subsequently, we discuss the importance of data analysis and signal detection schemes to achieve smaller instruments with low cost for SERS-based point-of-care technology developments. Finally, we review the main advantages and challenges of SERS-based biosensing and provide a brief outlook.

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“…Due to the ionic and coordination bonds, adsorption of clenbuterol and ractopamine on the AuNPs surface could decrease the negative charge of AuNPs and the stability of citrate-stabilized AuNPs against aggregation. 26 Besides, the N-donors have a stronger affinity with AuNPs than the carboxyl groups, because compounds with electron-rich nitrogen atoms easily bind onto the surface of AuNPs via Au-N bonds and shorten the average distance between AuNPs, which results in the aggregation of the AuNPs. 27 Thus an obvious color change of AuNPs from wine red to blue could be observed with the naked eye or a UV-vis spectrometer.…”

Section: Introductionmentioning

confidence: 99%

Visual Screening and Colorimetric Determination of Clenbuterol and Ractopamine Using Unmodified Gold Nanoparticles as Probe

Luo¹,

Liu²,

Gao³

et al. 2016

j nanosci nanotechnol

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In this paper, a sensitive method for the colorimetric detection of clenbuterol and ractopamine using citrate-stabilized gold nanoparticles (AuNPs) as probe was developed. The concentration of clenbuterol and ractopamine could be determined with naked eyes or a UV-vis spectrometer. By optimizing the influence of NaHSO₄ and incubation time, clenbuterol could be detected in the linear range of 0.1-4 µg/mL with the detection limit of 0.0158 µg/mL, and ractopamine could be detected in the linear range of 1-9 µg/mL with the detection limit of 0.0229 µg/mL. The proposed method could be successfully applied to detect clenbuterol and ractopamine in pig urines by a simple pre-treatment with excellent recoveries. The proposed colorimetric assay exhibits good reproducibility and accuracy, providing a simple and rapid method for the analysis of clenbuterol and ractopamine.

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Adsorption of Beta-Adrenergic Agonists Used in Sport Doping on Metal Nanoparticles: A Detection Study Based on Surface-Enhanced Raman Scattering

Cited by 75 publications

References 33 publications

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles

Fundamentals and applications of SERS-based bioanalytical sensing

Visual Screening and Colorimetric Determination of Clenbuterol and Ractopamine Using Unmodified Gold Nanoparticles as Probe

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