Adsorption of Bovine Hemoglobin by Sulfonated Polystyrene Nanospheres

Chicken ovalbumin (OVA) and lysozyme (LZ) are important biological products, which come from the egg‐white. However, the separation of OVA and LZ is realized by traditional salting‐out method needing large amount of ammonium sulfate, leading to lysozyme enzyme activity reducing. In this work, we used a tropine‐based ionic liquid (IL) hydrogel to separate OVA and LZ. The adsorption isotherms, thermodynamics and kinetics were investigated. The IL gel showed maximum adsorption capacity of 926 mg g−1 for OVA, nearly 152 % higher than that reported in other literature. Moreover, the secondary structure of desorbed OVA was confirmed by fluorescence spectroscopy (FL), circular dichroism spectra (CD) and differential scanning calorimetry (DSC). The material presented high OVA adsorption capacity, excellent OVA selectivity and good biocompatibility in OVA‐LZ protein system. It could be considered as a promising material for preferential protein adsorption, at least for separation of OVA and LZ.

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“…1] [22] and Langmuir adsorption equation [Eq. 2] [23] are two classic models of isothermal adsorption.…”

Section: Study Of Adsorption Isothermsmentioning

confidence: 99%

Tropine‐Based Ionic Liquid Gel for Adsorption of Protein Ovalbumin: High Capacity, Selectivity and Biocompatibility

Lin

Yao

et al. 2020

ChemistrySelect

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“…The purified protein is crucial for advancing life science technologies and has found extensive use in immunotherapy, medicinal therapy, food industry, and health detection [1][2][3]. Protein absorption and separation has been demonstrated to be one of the most efficient methods for obtaining highly purified proteins due to its high precision and convenient operation [4][5][6]. Currently, the conventional absorption and separation process is proceeded by microparticle media-packed chromatography columns.…”

Section: Introductionmentioning

confidence: 99%

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Lu,

Jiang,

Qiao

et al. 2024

Polymers

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Developing high-performance and low-cost protein purification materials is of great importance to meet the demands for highly purified proteins in biotechnological industries. Herein, a facile strategy was developed to design and construct high-efficiency protein absorption and separation media by combining aerogels’ molding techniques and impregnation processes. Poly (ethylene-co-vinyl alcohol) (EVOH) nanofibrous aerogels (NFAs) were modified by grafting butane tetracarboxylic acid (BTCA) over them in situ. This modification was carried out using polyphosphoric acid as a catalyst. The resulting EVOH/BTCA NFAs exhibited favorable comprehensive properties. Benefiting from the highly interconnected porous structure, good underwater compressive properties, and abundant absorption ligands, the obtained EVOH/BTCA NFAs possessed a high static absorption capacity of 1082.13 mg/g to lysozyme and a short absorption equilibrium time of about 6 h. A high saturated dynamic absorption capacity for lysozyme (716.85 mg/g) was also realized solely by gravity. Furthermore, EVOH/BTCA NFAs displayed excellent reusability, good acid and alkaline resistance, and unique absorption selectivity performance. The successful synthesis of such aerogels can provide a potential candidate for next-generation protein absorbents for bio-separation and purification engineering.

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Bacterial cellulose nanofibrous aerogels grafted with citric acid for absorption and separation of protein

Lu,

Jiang,

Wen

et al. 2023

Cellulose

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Adsorption of Bovine Hemoglobin by Sulfonated Polystyrene Nanospheres

Cited by 7 publications

References 47 publications

Tropine‐Based Ionic Liquid Gel for Adsorption of Protein Ovalbumin: High Capacity, Selectivity and Biocompatibility

Tropine‐Based Ionic Liquid Gel for Adsorption of Protein Ovalbumin: High Capacity, Selectivity and Biocompatibility

Butane Tetracarboxylic Acid Grafted on Polymeric Nanofibrous Aerogels for Highly Efficient Protein Absorption and Separation

Bacterial cellulose nanofibrous aerogels grafted with citric acid for absorption and separation of protein

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