Adsorption of bovine serum albumin on nanosized magnetic particles

Preparation and characteristics of novel porous hydrogel films based on chitosan and glycerophosphate

“…BSA (pI = 4.7, M w = 68 kDa) is an amphiphilic protein due to the presence of a NH 2 and a COOH group in its molecular structure. The pH of the environment has a great effect on BSA adsorption (Peng, Hidajat, & Uddin, 2004), and under the experimental condition (pH 7.4 buffer) in this study it carries a negative charge. While lysozyme (pI = 11, M w = 14.6 kDa), a globular protein, contains a large number of OH and NH 2 groups showed positive charge.…”

Immobilization of glucose oxidase and lactate dehydrogenase onto magnetic nanoparticles for bioprocess monitoring system

“…4. From the spectra for the magnetic nanoparticles, GOD-immobilized nanoparticles and LDH-immobilized nanoparticles, the peaks at 2,932 and 1,631 cm −1 might originate from amide groups (−NH 2 ) due to the use of concentrated ammonia solution during the co-precipitation of the Fe 2+ and Fe 3+ ions [34]. The increase of the peaks for the GOD-and LDH-immobilized nanoparticles at 2,361 cm −1 might be due to C≡N stretching vibrations, resulting from the binding of the magnetic nanoparticles to carbodiimide.…”

Protein aggregation in foam fractionation of bovine serum albumin: Effect of protein concentration

“…The results indicate that increasing C BSA made the structure of BSA more folded and the effect was significant when C BSA was lower than 0.20 g/L. At pH 7.0, the BSA molecules, of which their isoelectric point was pH 4.7, were negatively charged [23]. As C BSA increased, the BSA molecules became more crowded in the solution to enhance the intermolecular Coulomb repulsion forces, so the BSA structure had to 11 become more folded to decrease the molecular size [24].…”