Adsorption of fibrinogen on tantalum oxide, titanium oxide and gold studied by the QCM-D technique

Quantitative measurement of p53-p53 antibody interactions by quartz crystal microbalance: A modelsystem for immunochemical calibration

“…According to this model, increase in antibody protein coverage may lead to molecular cross linking which would result in surface film stiffening as indicated by R decreases (Figure 2(c)). Moreover, the ratio of anti-p53 IgG/p53 protein in the film (median value for the three antibody types = 0.18, see also Tables 2 and 3) suggests that one antibody molecule could be attached to several surface p53 proteins effectively cross linking them [19]. Hook et al [7] have shown earlier that protein film cross linking leads to a more rigid and compact film as reflected by lower values in the energy dissipation parameter D. On the other hand, the lower ratio of p53 antibody binding may also be due to steric hindrance of the exposed p53 protein epitopes.…”

Effects of dynamics of water molecules at hydrophilic polymer brush surfaces on protein adsorption behavior

“…The adsorbed amount of proteins from 100% fetal bovine serum (FBS) on the polymer brush surfaces at 37 °C was quantified using QCM-D measurement (Q-Sense, Gothenburg, Sweden) [14,15,17]. To measure the protein adsorption mass on the surface, the polymer brush layers were formed at the surface of QCM-D gold sensor.…”

“…Hydration state at the surfaces was evaluated by measurement of relaxation time and diffusion coefficient using proton nuclear magnetic resonance spectroscopy ( 1 H-NMR) [12,13]. Protein adsorption onto the surfaces was measured with a quartz crystal microbalance with dissipation monitoring (QCM-D) measurement [14,15]. Finally, the relationship between protein adsorption and dynamics of water was discussed.…”

Optical Detection of Protein Adsorption on Doped Titanium Surface

“…The structure and composition of the adsorbed protein layer determine the type and extent of the subsequent biological reactions, such as activation of coagulation and immune response and osseointegration (Nygren et al, 1997). Thus, the initially adsorbed protein layer is a factor determining the biocompatibility (Cai et al, 2006;Galli et al, 2001;Garguilo et al, 2004;Hemmersam et al, 2005;Kidoaki & Matsuda, 1999;Ma et al, 2007;Rouahi et al, 2006;Van De Keere et al, 2008;Wang et al, 2003), and also in recent years interest has been focused to preparation of hydrocarbons doped with Ti and used different methods to analyzing of biocompatibility for important proteins (Choukourov et al, 2008;Grinevich et al, 2009;Silvennoinen, Hason, Vetterl, Penttinen, Silvennoinen, Myller, Cernochova, Bartakova, Prachar & Cvrcek, 2010;Silvennoinen, Vetterl, Hason, Tuononen, Silvennoinen, Myller, Cvrcek, Vanek & Prachar, 2008). The production and application of doped titanium surfaces are under intensive research, and the results have shown the positive views on the adaptation of these materials as a biomaterial, as equal or even better than the bulk titanium.…”