Adsorption of Glucoamylase on DEAE‐Cellulose

Jach, M.; Sugier, H.

doi:10.1002/star.19830351206

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…Therefore, under the sake bulk conditions (pH 4.0-4.5), cationic adsorbents may be used for adsorption of A. species GA from crude sake materials. It has already been reported that cationic adsorbents, such as DEAE-cellulose, [8] polyethyleneimine-coated Sepabeads, [9] catalytic filamentous carbon coated alumina-selica, [10] and metal-chelated polymer beads [11] were used as an adsorbent for the GA. However, it is expected that these cationic adsorbents having ligands of high pK a cannot selectively remove GA from a GA solution containing organic acids such as lactic acid and acetic acid because they highly adsorb both GA and organic acid.…”

Section: Introductionmentioning

confidence: 99%

EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

Sakata

Kurogi

Kai

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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& To selective adsorption of glucoamylase (GA) from the raw materials of the unpasteurized sake, various polymer beads were prepared by immobilization of cationic or anionic functional groups onto cellulose beads (Cellufine). Under conditions which mimic sake (GA 500 lg mL À1 , ethanol 20 vol%, pH 4), Aspergillus species GA (As-GA) (pI 3.5) was strongly adsorbed by cationic Cellufines with anion-exchange capacity (AEC) of 0.5-1.3 meq g À1 , but Rhizopus sp. GA (pI 6.5) was strongly adsorbed by anionic adsorbents with cation-exchange capacity of 0.3-1.1 meq g À1 . The As-GA selectivity of the cationic Cellufines depended on their apparent pK a (pK a,app ) but not necessarily on their AEC. The Cellufine-poly(ethyleneimine) (pK a,app 7.9, AEC 0.4 meq g À1 ) was effective at adsorbing As-GA (>99%) in a sake model solution (GA 250 lg ml À1 , ethanol 20 vol%, 0.016 M acetate buffer, pH 4), but it also adsorbed organic acid, such as acetic acid. By contrast, Cellufine-poly(e-lysine) (pK a,app 7.3-7.4, AEC 0.6-1.0 meq g À1 ) selectively removed the As-GA from the sake-model solution without adsorption of the organic acid; the residual As-GA concentration was lower than 1%, and the recovery of organic acid was more than 99% as total acidity.

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Section: Introductionmentioning

confidence: 99%

EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

Sakata

Kurogi

Kai

et al. 2013

Journal of Liquid Chromatography & Related Technologies

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Comparative Evaluation of Immobilized Glucoamylases

László

Holló

1986

Starch Stärke

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Immobilized enzymes are used today in various industrial technologies, and further widespread application may by expected in the years to come. An essential precondition to this is to compare methods described in the literature for a given enzyme and recently developed immobilization techniques from aspects of economic efficiency. Comparison may be drawn on the basis of results reported in scientific papers. The study of various immobilized enzymes led to the conclusion that only material expenses can be accepted as comparative fundamental data. On the basis of this, immobilized enzymes may be described by calculation and comparison of the following three characteristic values: 1. Specific material costs of immobilization; 2. initial productivity of unit volume of the reactor, or total product formation of the immobilized enzyme applied for one half‐life; 3. costs of enzymes in unit product formation. Applicability of the method is presented in the evaluation of amyloglucosidase enzymes.

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The Properties of Glucoamylase Soluble and Immobilized on DEAE‐Cellulose. Part I. Kinetics and Thermodynamics of Enzyme Reaction

Przybyt

Sugier

1988

Starch Stärke

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Adsorption of Glucoamylase on DEAE‐Cellulose

Cited by 7 publications

References 11 publications

EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

Comparative Evaluation of Immobilized Glucoamylases

The Properties of Glucoamylase Soluble and Immobilized on DEAE‐Cellulose. Part I. Kinetics and Thermodynamics of Enzyme Reaction

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Adsorption of Glucoamylase on DEAE‐Cellulose

Cited by 7 publications

References 11 publications

EFFECT OF ADSORBENT pKa ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

EFFECT OF ADSORBENT pKa ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

Comparative Evaluation of Immobilized Glucoamylases

The Properties of Glucoamylase Soluble and Immobilized on DEAE‐Cellulose. Part I. Kinetics and Thermodynamics of Enzyme Reaction

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EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS

EFFECT OF ADSORBENT pK_a ON SELECTIVE REMOVAL OF GLUCOAMYLASE FROM UNPASTEURIZED SAKE MATERIALS