Adsorption of lipase on polypropylene powder

Gitlesen, Thomas; Bauer, Michael; Adlercreutz, Patrick

doi:10.1016/s0005-2760(96)00176-2

Cited by 158 publications

(108 citation statements)

References 18 publications

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“…3.1.1.3), which hydrolyze ester linkages of glycerides at water-oil interface. It is well known that lipases are the most widely used enzymes in organic synthesis and more than 20% biotransformation are performed with lipases (Gitlesen et al, 1997). In addition to their role in synthetic organic chemistry, these also find extensive applications in chemical, pharmaceutical, food and leather industries (Gulati et al, 2005;Gunstone, 1999).…”

Section: Introductionmentioning

confidence: 99%

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Liman¹,

Egwin²,

Vunchi³

et al. 2010

Nig J Bas App Sci

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ABSTRACT:The activity of lipase in fermented Africa locust bean, Castor seed, and Africa oil bean was determined. The peak lipase activity for fermented Africa locust bean, Castor seed, and African oil bean were 13.3x10 -3 µmol/sec, 7.2 x10 -3 µmol/sec and 10.6 x10 -3 µmol/sec at day 5, 4 and 4 respectively. The optimum temperature and pH were 30 0 C and 7.0 respectively for all the fermented seeds above. Increasing NaCl concentration decreases the activity of Lipase indicating that NaCl is an inhibitor of lipase. The effect of substrate concentration on lipase activity in the fermenting oil seeds shows the normal Michaelis-Menten curve. The K m for African locust bean is 0.065664mM, Castor seed is 0.067625mM, and African oil bean is 0.075848mM. While the V max are: 15.229x10 -3 µmol/sec, 14.787x10 -3 µmol/sec and 13.184x10 -3 µmol/sec respectively. The findings of this work indicates that fermented African locust bean, Castor seed and Africa oil bean could be a source of lipase for industrial application.

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Section: Introductionmentioning

confidence: 99%

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Liman¹,

Egwin²,

Vunchi³

et al. 2010

Nig J Bas App Sci

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“…An important characteristic of lipases is their ability not only to hydrolyze the ester bonds, trans-esterify triglycerides and resolve racemic mixture, but also to synthesize ester bonds in non-aqueous media (21,29). It is well known that lipases are the most widely used enzymes in organic synthesis and more than 20% biotransformations are performed with lipases (12). To date, a large number of lipases from bacteria and fungi have been extensively studied, both from the biochemical and from the genetic point of view (3,38,41).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9

Borkar¹,

Bodade²,

Rao³

et al. 2009

Braz. J. Microbiol.

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An extra cellular lipase was isolated and purified from the culture broth of Pseudomonas aeruginosa SRT 9 to apparent homogeneity using ammonium sulfate precipitation followed by chromatographic techniques on phenyl Sepharose CL-4B and Mono Q HR 5/5 column, resulting in a purification factor of 98 fold with specific activity of 12307.8 U/mg. The molecular weight of the purified lipase was estimated by SDS-PAGE to be 29 kDa with isoelectric point of 4.5. Maximum lipase activity was observed in a wide range of temperature and pH values with optimum temperature of 55ºC and pH 6.9. The lipase preferably acted on triacylglycerols of long chain (C14-C16) fatty acids. The lipase was inhibited strongly by EDTA suggesting the enzyme might be metalloprotein. SDS and metal ions such as Hg , Ag 2+ and Fe 2+ decreased the lipase activity remarkedly. Its marked stability and activity in organic solvents suggest that this lipase is highly suitable as a biotechnological tool with a variety of applications including organo synthetic reactions and preparation of enantiomerically pure pharmaceuticals. The Km and Vmax value of the purified enzyme for triolein hydrolysis were calculated to be 1.11 mmol/L and 0.05 mmol/L/min respectively.

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“…Lipases are being exploited due to their low cost of extraction, thermal and pH stability, substrate specificity and activity in organic solvents. Lipases are the most widely used enzymes in organic synthesis and more than 20% biotransformations are performed with lipases (Gitleson et al, 1997). The field of industrial enzymes is now experiencing major R and D initiatives, resulting in both the development of a number of new products and in improvement in the process and performance of several existing products.…”

Section: Applications Of Lipasesmentioning

confidence: 99%

Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site

Patel¹,

Mistry²,

Desai³

et al. 2016

Int.J.Curr.Microbiol.App.Sci

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Adsorption of lipase on polypropylene powder

Cited by 158 publications

References 18 publications

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Lipase Activity in Fermented Oil Seeds of Africa Locust Bean, (Parkia Biglobosa), Castor Seeds (Ricinu Communis) and African Oil Bean (Pentaclethra Macrophylla).

Purification and characterization of extracellular lipase from a new strain: Pseudomonas aeruginosa SRT 9

Isolation and Characterization of Lipase producing Bacteria from Vegetable Oil Spillage Site

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