Adsorption of lysozyme and trypsin onto mesoporous silica materials

Yang, Ji; Daehler, Antje; Stevens, Geoffrey W.; O’Connor, Andrea J.

doi:10.1016/s0167-2991(03)80498-6

Cited by 16 publications

(8 citation statements)

References 20 publications

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“…The adsorption of peptides on surfaces has been recognized to be critical in numerous fields such as biomaterials, biological separations, and biosensors. In this context, the exchange of proteins at the surface with molecules in the bulk has been investigated experimentally in the absence of a porous structure. , Studies concerning the adsorption of proteins on molecular sieves such as MCM-41 have also been reported, the problems addressed here being the efficiency of immobilization as a function of pH, concentration of the solution, and pore size, , and the catalytic activity in the case that the protein is an enzyme. − …”

Section: Introductionmentioning

confidence: 99%

NMR Study of the Adsorption−Desorption Kinetics of Dissolved Tetraalanine in MCM-41 Mesoporous Material

et al. 2005

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In this study we show how deuterium magic-angle spinning NMR spectroscopy can be used to investigate the adsorption-desorption kinetics of molecules in solution at surface-liquid interfaces. An aqueous solution of deuterium-labeled tetraalanine is inserted in the pores of MCM-41 mesoporous material, and its 2H MAS NMR spectrum is measured as a function of temperature and fraction of filling of the pores. Prior to this study, the different types of water in MCM-41 are characterized as a function of water loading of the pores. Analysis of 2H MAS sideband line shapes enabled the determination of the adsorption and desorption rates and the activation energies of desorption.

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Section: Introductionmentioning

confidence: 99%

NMR Study of the Adsorption−Desorption Kinetics of Dissolved Tetraalanine in MCM-41 Mesoporous Material

et al. 2005

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“…Yang, Daehler, Stevens, and O'Connor (2003) reported the adsorption kinetics of lysozyme on MCM-41 and SBA-15. For both materials, equilibrium was reached within about 48 h, with somewhat faster kinetics seen for the uptake on SBA-15 (Fig.…”

Section: Adsorption Capacity and Kineticsmentioning

confidence: 99%

“…Lysozyme adsorption kinetics on MCM-41("Mobil Composition of Matter 41") represented by the triangles (pore diameter = 3.3 nm) and SBA-15 ("Santa Barbara Amorphous 15") represented by the squares (pore diameter = 4.9 nm)(Yang et al, 2003).…”

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confidence: 99%

Hierarchical mesoporous silica materials for separation of functional food ingredients — A review

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Woonton

Gee

et al. 2008

Innovative Food Science & Emerging Technologies

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“…Continuous loading and realtime measurements of BSA concentration in the outlet minimized the errors associated with mixing and washing in batch mode, thus the effectiveness and efficiency of BSA loading on MPS in a scalable, continuous process was displayed. Moreover, continuous loading also reduced the time needed to reach equilibrium adsorption, 28 which was less than 2 h on average. Figures 3 and 4 show the characteristics of the MPS before and after BSA adsorption.…”

Section: Adsorption Studiesmentioning

confidence: 99%