Adsorption of protein antigen to the cationic liposome adjuvant CAF®01 is required for induction of Th1 and Th17 responses but not for antibody induction

Wørzner, Katharina; Hvannastein, Jóhanna; Schmidt, S.; Foged, Camilla; Rosenkrands, Ida; Pedersen, Gabriel Kristian; Christensen, Dennis

doi:10.1016/j.ejpb.2021.05.020

Cited by 13 publications

(11 citation statements)

References 39 publications

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“…Furthermore, only at 7 days after immunization, the levels of IgG and SIgA of the mice immunized with SeL or HiSeL and vaccine had a better promotion effect than those immunized with L. brevis 23017 and vaccine, whereas the mice immunized with L. brevis 23017 and vaccine were slightly increased when compared with the mice immunized with vaccine alone, suggesting that SeL and HiSeL induced immune response faster than L. brevis 23017, which also shows that SeL and HiSeL effectively made up for the weaknesses of slow IgG antibody production ( 58 ) and no SIgA production of alum adjuvant. We speculate that the reason for inducing a more rapid antibody production is that lactic acid bacteria and selenium have a synergistic effect in improving the antibody level ( 59 ), and then, they work together to accelerate the production of IgG antibody by B lymphocytes and the increment of SIgA antibody by the intestinal mucosa.…”

Section: Discussionmentioning

confidence: 96%

Nano selenium-enriched probiotic Lactobacillus enhances alum adjuvanticity and promotes antigen-specific systemic and mucosal immunity

Liu

Sun²,

Sun³

et al. 2023

Front. Immunol.

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Nano selenium-enriched probiotics have been identified to improve immune responses, such as alleviating inflammation, antioxidant function, treatment of tumors, anticancer activity, and regulating intestinal flora. However, so far, there is little information on improving the immune effect of the vaccine. Here, we prepared nano selenium-enriched Levilactobacillus brevis 23017 (SeL) and heat-inactivated nano selenium-enriched L. brevis 23017 (HiSeL) and evaluated their immune enhancing functions on the alum-adjuvanted, inactivated Clostridium perfringens type A vaccine in mouse and rabbit models, respectively. We found that SeL enhanced immune responses of the vaccine by inducing a more rapid antibody production, eliciting higher immunoglobulin G (IgG) antibody titers, improving secretory immunoglobulin A (SIgA) antibody level and cellular immune response, and regulating Th1/Th2 immune response, thus helping to induce better protective efficacy after challenge. Moreover, we confirmed that the immunoenhancement effects are related to regulating oxidative stress, cytokine secretion, and selenoprotein expression. Meanwhile, similar effects were observed in HiSeL. In addition, they show enhanced humoral immune response at 1/2 and 1/4 standard vaccine doses, which confirms their prominent immune enhancement effect. Finally, the effect of improving vaccine immune responses was further confirmed in rabbits, which shows that SeL stimulates the production of IgG antibodies, generates α toxin–neutralizing antibodies rapidly, and reduces the pathological damage to intestine tissue. Our study demonstrates that nano selenium-enriched probiotics improve the immune effect of the alum adjuvants vaccine and highlight its potential usage in remedying the disadvantages of alum adjuvants.

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Section: Discussionmentioning

confidence: 96%

Nano selenium-enriched probiotic Lactobacillus enhances alum adjuvanticity and promotes antigen-specific systemic and mucosal immunity

Liu

Sun²,

Sun³

et al. 2023

Front. Immunol.

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“…In the case of the CAF®01 formulation, we also cannot rule out the effect of the antigen adsorption into the liposome formulation. Indeed, a recent study demonstrated that changes of the electrostatic interactions between antigens and CAF®01 can lead to different immune responses [48] . Thus, the authors associated efficient Th1/Th17-type responses to higher adsorption rates of the antigen into the vaccine formulation, in inverse correlation with antibody titers.…”

Section: Discussionmentioning

confidence: 99%

Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDA

et al. 2023

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“…The optimal dose of an antigen, whether protein-, peptide- or nucleic-acid-based, varies from antigen to antigen and (unfortunately) from species to species. Most important, however, is that the optimum antigen dose is not the same for all vaccine technologies, and for some technologies, it has a major impact on the T/B cell induction balance, as illustrated with CAF01 by Wørzner et al [ 38 ], meaning that antigen dose optimization should be linked to the expected correlates of protection for the vaccine. Sampling timepoint.…”

Section: What To Think About When Designing Comparative Vaccine Techn...mentioning

confidence: 99%

Head-to-Head Comparison of Novel Vaccine Technologies Comes with a Minefield of Challenges

Christensen

2023

Pharmaceutics

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Modern vaccine development is having a golden period, with a variety of novel subunit technologies being introduced into clinical development in recent years. This opens the opportunity to find the best platform to use for novel vaccine antigen candidates through head-to-head comparative studies. Seldom appreciated is, however, the fact that these different technologies often do not have the same optimal antigen dose ratio, prime-boost regime and peak timepoint for measuring immunity. Instead, the preclinical studies that make the basis for platform selection use standard protocols not optimized for individual vaccines and fail to make selection on an informed basis. Here, I discuss the opportunities we have to optimize vaccine platform technologies through a better understanding of vaccine priming kinetics, the optimal antigen dose and sampling time and location.

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Adsorption of protein antigen to the cationic liposome adjuvant CAF®01 is required for induction of Th1 and Th17 responses but not for antibody induction

Cited by 13 publications

References 39 publications

Nano selenium-enriched probiotic Lactobacillus enhances alum adjuvanticity and promotes antigen-specific systemic and mucosal immunity

Nano selenium-enriched probiotic Lactobacillus enhances alum adjuvanticity and promotes antigen-specific systemic and mucosal immunity

Immune responses following neonatal vaccination with conserved F4 fragment of VtaA proteins from virulent Glaesserella parasuis adjuvanted with CAF®01 or CDA

Head-to-Head Comparison of Novel Vaccine Technologies Comes with a Minefield of Challenges

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