Adsorption of temperate phages of<i>Lactobacillus delbrueckii</i>strains and phage resistance linked to their cell diversity

Trucco, Verónica Milagros; Reinheimer, Jorge Alberto; Quiberoni, Andrea del Luján; Suárez, Viviana Beatriz

doi:10.1111/j.1365-2672.2011.04945.x

Cited by 12 publications

(5 citation statements)

References 36 publications

(79 reference statements)

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“…Nevertheless, Suárez et al. (2008) and Trucco et al. (2011) obtained phage‐resistant derivatives from Lact.…”

Section: Discussionmentioning

confidence: 99%

Isolation and phenotypic characterization of Lactobacillus casei and Lactobacillus paracasei bacteriophage-resistant mutants

Capra¹,

Mercanti²,

Rossetti³

et al. 2011

Journal of Applied Microbiology

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Aims: To isolate and characterize bacterial strains derived from Lactobacillus casei and Lactobacillus paracasei strains and resistant to phage MLC‐A. Methods and Results: Two of nine assayed strains rendered resistant mutants with recovery efficiencies of 83% (Lact. paracasei ATCC 27092) and 100% (Lact. casei ATCC 27139). DNA similarity coefficients (RAPD–PCR) confirmed that no significant genetic changes occurred while obtaining resistant mutants. Neither parent nor mutant strains spontaneously released phages. Phage‐resistant mutants were tested against phages PL‐1, J‐1, A2 and MLC‐A8. Lactobacillus casei ATCC 27092 mutants showed, overall, lower phage resistance than Lact. paracasei ATCC 27092 ones, but still higher than that of the parent strain. Lactobacillus paracasei ATCC 27092 mutants moderately adsorbed phage MLC‐A only in calcium presence, although their parent strain successfully did it with or without calcium. Adsorption rates for Lact. casei ATCC 27139 and its mutants were highly influenced by calcium. Again, phage adsorption was higher on the original strain. Conclusions: Several isolates derived from two Lact. casei and Lact. paracasei strains showed resistance to phage MLC‐A but also to other Lact. casei and Lact. paracasei phages. Significance and Impact of the Study: This study highlights isolation of spontaneous bacteriophage‐resistant mutants from Lact. casei and Lact. paracasei as a good choice for use in industrial rotation schemes.

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“…Nevertheless, Suárez et al. (2008) and Trucco et al. (2011) obtained phage‐resistant derivatives from Lact.…”

Section: Discussionmentioning

confidence: 99%

Isolation and phenotypic characterization of Lactobacillus casei and Lactobacillus paracasei bacteriophage-resistant mutants

Capra¹,

Mercanti²,

Rossetti³

et al. 2011

Journal of Applied Microbiology

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“…MRS medium supplemented with 10 mM CaCl 2 and 10 mM MgSO 4 (MRS-Ca-Mg), only with 10 mM CaCl 2 (MRS-Ca) (Ebrecht et al 2010;Trucco et al 2011;Mariángeles et al 2012) and only with 10 mM MgSO 4 (MRS-Mg) were used. Solid and soft agar media were MRS-Ca-Mg supplemented with 1.5 % and 0.5-0.6 % agar, respectively.…”

Section: Bacterial Strains and Culture Conditionsmentioning

confidence: 99%

Isolation and Characterization of a Novel Virulent Phage of Lactobacillus casei ATCC 393

Zhang

Jiao

et al. 2015

Food Environ Virol

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A new virulent phage (Lcb) of Lactobacillus casei ATCC 393 was isolated from Chinese sauerkraut. It was specific to L. casei ATCC 393. Electron micrograph revealed that it had an icosahedral head (60.2 ± 0.8 nm in diameter) and a long tail (251 ± 2.6 nm). It belonged to the Siphoviridae family. The genome of phage Lcb was estimated to be approximately 40 kb and did not contain cohesive ends. One-step growth kinetics of its lytic development revealed latent and burst periods of 75 and 45 min, respectively, with a burst size of 16 PFU per infected cell. The phage was able to survive in a pH range between 4 and 11. However, a treatment of 70 °C for 30 min and 75% ethanol or isopropanol for 20 min was observed to inactivate phage Lcb thoroughly. The presence of both Ca(2+) and Mg(2+) showed a little influence on phage adsorption, but they were indispensable to gain complete lysis and improve plaque formation. The adsorption kinetics were similar on viable or nonviable cells, and high adsorption rates maintained between 10 and 37 °C. The highest adsorption rate was at 30 °C. This study increased the knowledge on phages of L. casei. The characterization of phage Lcb is helpful to establish a basis for adopting effective strategies to control phage attack in industry.

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“…Trucco et al found that the maximum adsorption rates of phages CB1/204 and Cb1342 were achieved at pH 4-7, and when the pH increased to 8, the adsorption rates of both phages decreased [45]. The adsorption of L. paracasei phages ΦiLp84 and ΦiLp1308 was strongly inhibited at pH 4 and relatively stable at pH 5-8 [29].…”

Section: Influence Of Ph On Phage Adsorptionmentioning

confidence: 99%

Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

Gao

Zhang

et al. 2023

Foods

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Limosilactobacillus fermentum is a bacterium widely used in food production, medicine, and industrial fermentation. However, fermentation could fail due to phage contamination. L. fermentum bacteriophage LFP02 can be induced from L. fermentum IMAU 32579 using mitomycin C. To better understand the characteristics of this phage, its physiological and genomic characteristics were evaluated. The results showed that its optimal multiplicity of infection was 0.01, and the burst size was 148.03 ± 2.65 pfu/infective center. Compared to temperature, pH had a more obvious influence on phage viability, although its adsorption capacity was not affected by the divalent cations (Ca2+ and Mg2+) or chloramphenicol. Its genome size was 43,789 bp and the GC content was 46.06%, including 53 functional proteins. Compared to other L. fermentum phages, phage LFP02 had chromosome deletion, insertion, and inversion, which demonstrated that it was a novel phage. This study could expand the knowledge of the biological characteristics of L. fermentum bacteriophages and provide some theoretical basis for bacteriophage prevention during fermentation.

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Adsorption of temperate phages ofLactobacillus delbrueckiistrains and phage resistance linked to their cell diversity

Cited by 12 publications

References 36 publications

Isolation and phenotypic characterization of Lactobacillus casei and Lactobacillus paracasei bacteriophage-resistant mutants

Isolation and phenotypic characterization of Lactobacillus casei and Lactobacillus paracasei bacteriophage-resistant mutants

Isolation and Characterization of a Novel Virulent Phage of Lactobacillus casei ATCC 393

Characteristics and Whole-Genome Analysis of Limosilactobacillus fermentum Phage LFP02

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