Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity

Zhang, Su‐Chun; Ge, Bin; Duncan, Ian D.

doi:10.1073/pnas.96.7.4089

Cited by 189 publications

(109 citation statements)

References 39 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…Multiple approaches have been developed for OPC purification; each has its own merits. Compared to other OPC isolation methods such as costly immunopanning 5,9,10 and technically demanding differential gradient centrifugation 7,8,12 , the method for OPC preparation through oligosphere generation from both mouse and rat neural progenitor cells is simple and straightforward 20,21,26 . It can be easily followed by a researcher without prior OPC isolation experience.…”

Section: Anticipated Resultsmentioning

confidence: 99%

Isolation and culture of rat and mouse oligodendrocyte precursor cells

et al. 2007

View full text Add to dashboard Cite

Section: Anticipated Resultsmentioning

confidence: 99%

Isolation and culture of rat and mouse oligodendrocyte precursor cells

et al. 2007

View full text Add to dashboard Cite

“…The presence of Ulip6/CRMP5and Ulip2/CRMP2 in oligodendrocytes might be related to different functions of these cells, depending on their localization or degree of maturation. In fact, some Ulip/CRMPexpressing cells might be oligodendrocyte progenitors, retaining the capacity of migration/differentiation in adult brain (Zhang et al, 1999). However, differentiated oligodendrocytes expressed Ulip6/CRMP5, because loops around axons belonging to myelinating oligodendrocytes were labeled by anti-Ulip6/CRMP5 antibodies.…”

Section: Molecular and Cellular Characterization Of Ulip6/crmp5mentioning

confidence: 99%

Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Ricard¹,

Rogemond²,

Charrier³

et al. 2001

J. Neurosci.

124

104

View full text Add to dashboard Cite

The Unc-33-like phosphoprotein/collapsin response mediator protein (Ulip/CRMP) family consists of four homologous phosphoproteins considered crucial for brain development. Autoantibodies produced against member(s) of this family by patients with paraneoplastic neurological diseases have made it possible to clone a fifth human Ulip/CRMP and characterize its cellular and anatomical distribution in developing brain. This protein, referred to as Ulip6/CRMP5, is highly expressed during rat brain development in postmitotic neural precursors and in the fasciculi of fibers, suggesting its involvement in neuronal migration/differentiation and axonal growth. In the adult, Ulip6/ CRMP5 is still expressed in some neurons, namely in areas that retain neurogenesis and in oligodendrocytes in the midbrain, hindbrain, and spinal cord. Ulip2/CRMP2 and Ulip6/CRMP5 are coexpressed in postmitotic neural precursors at certain times during development and in oligodendrocytes in the adult. Because Ulip2/CRMP2 has been reported to mediate semaphorin-3A (Sema3A) signal in developing neurons, in studies to understand the function of Ulip6/CRMP5 and Ulip2/ CRMP2 in the adult, purified adult rat brain oligodendrocytes were cultured in a Sema3A-conditioned medium. Oligodendrocytes were found to have Sema3A binding sites and to express neuropilin-1, the major Sema3A receptor component. In the presence of Sema3A, these oligodendrocytes displayed a dramatic reduction in process extension, which was reversed by removal of Sema3A and prevented by anti-neuropilin-1, antiUlip6/CRMP5, anti-Ulip2/CRMP2 antibodies, or VEGF-165, another neuropilin-1 ligand. These results indicate the existence in the adult brain of a Sema3A signaling pathway that modulates oligodendrocyte process extension mediated by neuropilin-1, Ulip6/CRMP5, and Ulip2/CRMP2, and they open new fields of investigation of neuron/oligodendrocyte interactions in the normal and pathological brain.

show abstract

“…To migrate widely throughout the CNS, OPCs need to respond to various growth factors (Barres and Raff, 1999;Miller, 2002). In the adult brain, OPCs may also be guided to sites of injury, where they contribute to myelin repair (Blakemore and Keirstead, 1999;Zhang et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Cdk5 Phosphorylation of WAVE2 Regulates Oligodendrocyte Precursor Cell Migration through Nonreceptor Tyrosine Kinase Fyn

Miyamoto¹,

Yamauchi²,

Tanoue³

2008

J. Neurosci.

109

100

View full text Add to dashboard Cite

Myelin formation of the CNS is a complex and dynamic process. Before the onset of myelination, oligodendrocytes (OLs), the myelinforming glia of the CNS, proliferate and migrate along axons. Little is known about the molecular mechanisms underlying the early myelination processes. Here, we show that platelet-derived growth factor (PDGF), the crucial physiological ligand in early OL development, controls the migration of oligodendrocyte precursor cells (

show abstract

Adult brain retains the potential to generate oligodendroglial progenitors with extensive myelination capacity

Cited by 189 publications

References 39 publications

Isolation and culture of rat and mouse oligodendrocyte precursor cells

Isolation and culture of rat and mouse oligodendrocyte precursor cells

Isolation and Expression Pattern of Human Unc-33-Like Phosphoprotein 6/Collapsin Response Mediator Protein 5 (Ulip6/CRMP5): Coexistence with Ulip2/CRMP2 in Sema3A- Sensitive Oligodendrocytes

Cdk5 Phosphorylation of WAVE2 Regulates Oligodendrocyte Precursor Cell Migration through Nonreceptor Tyrosine Kinase Fyn

Contact Info

Product

Resources

About