Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution

Goldstein, Bradley J.; Hare, Joshua M.; Lieberman, Seth; Casiano, Roy R.

doi:10.1002/alr.21153

Cited by 24 publications

(34 citation statements)

References 20 publications

(30 reference statements)

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“…[Kim et al, 2012]. They have also been found in the niche of olfactory mucosa which is adjacent to the respiratory mucosa [Goldstein et al, 2013]. Our previous work also indicated the existence of EMSCs in the lamina propria of the rat respiratory mucosa.…”

Section: Introductionmentioning

confidence: 71%

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Liu

Chen

Zhang

et al. 2013

Cells Tissues Organs

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Fibrin has been widely used in wound healing. However, its benefit for spinal cord injury (SCI) is limited. In this study, we investigated the impact of fibrin scaffolds containing ectomesenchymal stem cells (EMSCs) on histological and behavioral recovery after SCI and compared it with fibrin alone. To achieve this, EMSCs derived from adult rat nasal respiratory mucosa were cultured, characterized and transfected with green fluorescent protein adenovirus before transplantation. Then, Sprague-Dawley host rats were randomly assigned into four groups: the control group (laminectomy); the SCI group (laminectomy and transection of spinal cords); the fibrin group (fibrin was transplanted immediately after SCI), and the fibrin cell (FC) group (fibrin scaffolds containing EMSCs were transplanted after SCI). Three days after the operation, a TUNEL assay indicated less apoptotic cells in the FC group than in the fibrin group. Two weeks after SCI, fluorescence staining demonstrated not only the survival and migration of EMSCs into the lesion sites, but also a higher number of nerve fibers in the FC group than in the fibrin group. Histological examination including immunohistochemistry and transmission electron microscopy 12 weeks after the operation showed more nerve fibers and a thicker myelin sheath in the FC group compared to the fibrin group. Western blotting confirmed these morphological results. Consistent with the histological results, Basso, Beattie and Bresnahan locomotor scores of the FC group were higher than those of the fibrin group. These results suggest that fibrin scaffolds containing EMSCs can improve the behavioral and histological recovery after SCI better than fibrin alone.

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Section: Introductionmentioning

confidence: 71%

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Liu

Chen

Zhang

et al. 2013

Cells Tissues Organs

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“…This raises the prospects of (a) exploiting the neurogenic properties of olfactory tissue or (b) possibly delivering novel therapies directly to olfactory mucosa. However, despite the relative ease with which nasal lamina propria mesenchymal-like cells can be propagated in vitro (Murrell et al, 2005;Goldstein et al, 2013), techniques to efficiently expand OE basal stem and progenitor cells from adult mammals have remained challenging (Krolewski et al, 2011). Thus, efforts to better define the biology of adult olfactory stem and progenitors cells are significant.…”

Section: Translational Considerationsmentioning

confidence: 99%

Adult c‐Kit(+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons

Goldstein

Goss

Hatzistergos

et al. 2014

J of Comparative Neurology

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The olfactory epithelium houses chemosensory neurons, which transmit odor information from the nose to the brain. In adult mammals, the olfactory epithelium is a uniquely robust neuroproliferative zone, with the ability to replenish its neuronal and non-neuronal populations due to the presence of germinal basal cells. The stem and progenitor cells of these germinal layers, and their regulatory mechanisms, remain incompletely defined. Here we show that progenitor cells expressing c-Kit, a receptor tyrosine kinase marking stem cells in a variety of embryonic tissues, are required for maintenance of the adult neuroepithelium. Mouse genetic fate-mapping analyses show that embryonically, a c-Kit (+) population contributes to olfactory neurogenesis. In adults under conditions of normal turnover, there is relatively sparse c-Kit (+) progenitor cell (ckPC) activity. However, after experimentally induced neuroepithelial injury, ckPCs are activated such that they reconstitute the neuronal population. There are also occasional non-neuronal cells found to arise from ckPCs. Moreover, the selective depletion of the ckPC population, utilizing temporally controlled targeted diphtheria toxin A expression, results in failure of neurogenesis after experimental injury. Analysis of this model indicates that most ckPCs reside among the globose basal cell populations and act downstream of horizontal basal cells, which can serve as stem cells. Identification of the requirement for olfactory c-Kit expressing progenitors in olfactory maintenance provides new insight into the mechanisms involved in adult olfactory neurogenesis. Additionally, we define an important and previously unrecognized site of adult c-Kit activity.

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“…New nerves generate from GBS in the olfactory epithelium, which are guided to their correct position in the olfactory bulb by OECs in the olfactory mucosa. GBCs, derived from HBCs, were initially thought to be the exclusive source of ON and support cells [74] but it is currently known that MSCs are also capable of producing neurons in vitro [75].…”

Section: General Featuresmentioning

confidence: 99%

“…The olfactory epithelium or olfactory bulb tissue sample is then cut into small pieces, digested with collagenase and DNase [80,82] or dispase [78] to separate the cells from their extracellular matrix. Then, they are cultured in lasks containing appropriate medium like Dulbecco's modiied Eagle's medium or HAM´s F-12 supplemented with fetal calf serum or fetal bovine serum, antibiotics, antifungals and growth factors [75]. After removal of non-adherent cells, the remaining cells can be trypsinized, expanded [82,88] and then banked in liquid nitrogen or induced to form for differentiation.…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

“…Phenotypic analysis reveals the presence of Stro-1 [75][76][77], CD29 [80,88], CD44 [80,88], CD49b [90], CD54 [13,77,82], CD73 [77,82,90], CD90 [13, 75-78, 80, 82, 88, 90], CD105 [13, 75-78, 80, 82], CD106 [77,78] and CD166 [77,78,80,82,90] markers and the absence of haematopoietic stem cell markers such as CD34 [13, 75-78, 80, 88, 90], CD45 [13, 75-78, 80, 88] and CD11b [88]. Strangely, one work indicates lack of Stro-1 [82].…”

Section: Isolation Characterization and Diferentiationmentioning

confidence: 99%

See 1 more Smart Citation

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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The peripheral nerve injury after trauma is a common occurrence in both human and veterinary medicine and has severe consequences for the survival and quality of life of the patients. Despite the continuous eforts and the creation of diverse medical and surgical techniques, the harmful efects of this type of injury are far from being overcome. Regenerative medicine has been growing in the scientiic milieu as a new therapeutic approach for diferent situations. Among the cell-based therapies explored, the mesenchymal stem cells are evidenced by their features, versatility and potential applications. The olfactory mucosa mesenchymal stem cells, components of the olfactory system and identiied in the lamina propria, were newly identiied and are still undergoing characterization, appearing as a new promise in the regenerative therapy of several tissues but with special emphasis on the nervous system in general and the peripheral nervous system in particular, for which they appear to have special regenerative aptitude.

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Adult human nasal mesenchymal stem cells have an unexpected broad anatomic distribution

Abstract: The unanticipated broad anatomic distribution of nasal MSCs has implications for cell-based therapy research.

Cited by 24 publications

References 20 publications

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Fibrin Scaffolds Containing Ectomesenchymal Stem Cells Enhance Behavioral and Histological Improvement in a Rat Model of Spinal Cord Injury

Adult c‐Kit(+) progenitor cells are necessary for maintenance and regeneration of olfactory neurons

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

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