Adult multipotent stromal cell cryopreservation: Pluses and pitfalls

Duan, Wei; Lopez, Mandi J.; Hicok, Kevin C.

doi:10.1111/vsu.12730

Cited by 19 publications

(19 citation statements)

References 69 publications

(184 reference statements)

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“…The MSCs are cooled at −1°C/min down to −80°C and then transferred into the liquid nitrogen tank. And the MSCs can be used after thawed in complete medium at 37°C [ 37 ]. Although this conventional cryopreservation method increases the access and availability of MSCs, there remain great challenges which need to be overcome.…”

Section: Discussionmentioning

confidence: 99%

Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Chen

Deng

et al. 2018

Oxidative Medicine and Cellular Longevity

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Nucleus pulposus-derived mesenchymal stem cells (NPMSCs) have shown a good prospect in the regeneration of intervertebral disc (IVD) tissues. However, fresh NPMSCs are not always readily available for basic research and clinical applications. Therefore, there is a need for an effective long-term cryopreservation method for NPMSCs. The aim of this study was to determine whether adding icariin (ICA) to the conventional cryoprotectant containing dimethyl sulfoxide (DMSO) had a better cryoprotective effect for NPMSCs. The results showed that the freezing solution containing ICA along with DMSO significantly increased the postthawed cell viability, decreased the apoptosis rate, improved cell adherence, and maintained the mitochondrial functions, as compared to the freezing solution containing DMSO alone. And the inhibition of oxidative stress and upregulation of heat shock proteins (HSPs) in the presence of ICA also confirmed the beneficial effect of ICA. Furthermore, ICA had no cytotoxicity and did not alter the characteristics of postthawed NPMSCs. In conclusion, these results suggested that the addition of ICA to the conventional freezing medium could improve the viability and function of the cryopreserved human NPMSCs and provided an optimal formulated freezing solution for human NPMSC cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Chen

Deng

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…Cells in culture were subjected to enzymatic detachment with 0.25% trypsin-EDTA solution, collection and automatic counting. OM-MSCs were cryopreserved with culture medium and 10% Dimethyl sulfoxide (DMSO) (SigmaAldrich®) [49] in cryovials with at least 1 × 10 6 cells. e cryovials were then transferred to a freezing container with Isopropyl alcohol (Nalgene® Mr. Frosty®, ermoScientific®) for slow freezing (−1°C/minute) at −80°C.…”

Section: Cryopreservation and Awing Om Tissue Fragmentsmentioning

confidence: 99%

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

Alvites

Branquinho

Amorim

et al. 2020

International Journal of Cell Biology

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Stem/stromal cell-based therapies are a branch of regenerative medicine and stand as an attractive option to promote the repair of damaged or dysfunctional tissues and organs. Olfactory mucosa mesenchymal stem/stromal cells have been regarded as a promising tool in regenerative therapies because of their several favorable properties such as multipotency, high proliferation rate, helpful location, and few associated ethical issues. These cells are easily accessible in the nasal cavity of most mammals, including the rat, can be easily applied in autologous treatments, and do not cope with most of the obstacles associated with the use of other stem cells. Despite this, its application in preclinical trials and in both human and animal patients is still limited because of the small number of studies performed so far and to the nonexistence of a standard and unambiguous protocol for collection, isolation, and therapeutic application. In the present work a validation of a protocol for isolation, culture, expansion, freezing, and thawing of olfactory mucosa mesenchymal stem/stromal cells was performed, applied to the rat model, as well as a biological characterization of these cells. To investigate the therapeutic potential of OM-MSCs and their eventual safe application in preclinical trials, the main characteristics of OMSC stemness were addressed.

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“…In veterinary medicine, everything regarding tissue sampling, MSC culture, expansion, and cryopreservation is essentially similar to what is commonly applied in human medicine [7][8][9]. A summary panel of the main procedure is shown in Figure 1.…”

Section: Mesenchymal Stromal Cells and Veterinary Regenerative Medicimentioning

confidence: 99%

“…In association, FBS is used as a source of protein with synergic cryoprotectant effect [38]. A mixture of DMSO and FBS is considered as the standard cryo-protectant in veterinary medicine, even if the oncogenic and xenogeneic properties of DMSO and FBS, respectively, may alter cells and impact in vitro and in vivo behaviour after implantation [8].…”

Section: Cryopreservationmentioning

confidence: 99%

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Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?

Mocchi

Dotti

Bue³

et al. 2020

Cells

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Regenerative medicine aims to restore the normal function of diseased or damaged cells, tissues, and organs using a set of different approaches, including cell-based therapies. In the veterinary field, regenerative medicine is strongly related to the use of mesenchymal stromal cells (MSCs), which belong to the body repair system and are defined as multipotent progenitor cells, able to self-replicate and to differentiate into different cell types. This review aims to take stock of what is known about the MSCs and their use in the veterinary medicine focusing on clinical reports on dogs and horses in musculoskeletal diseases, a research field extensively reported in the literature data. Finally, a perspective regarding the use of the secretome and/or extracellular vesicles (EVs) in the veterinary field to replace parental MSCs is provided. The pharmaceuticalization of EVs is wished due to the realization of a Good Manufacturing Practice (GMP product suitable for clinical trials.

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Adult multipotent stromal cell cryopreservation: Pluses and pitfalls

Cited by 19 publications

References 69 publications

Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Icariin Improves the Viability and Function of Cryopreserved Human Nucleus Pulposus‐Derived Mesenchymal Stem Cells

Rat Olfactory Mucosa Mesenchymal Stem/Stromal Cells (OM-MSCs): A Characterization Study

Veterinary Regenerative Medicine for Musculoskeletal Disorders: Can Mesenchymal Stem/Stromal Cells and Their Secretome Be the New Frontier?

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