Adult plant stem rust resistance in durum wheat Glossy Huguenot: mapping, marker development and validation

Abstract

An F 3 population from a Glossy Huguenot (GH)/Bansi cross used in a previous Australian study was advanced to F 6 for molecular mapping of adult plant stem rust resistance. Maturity differences among F 6 lines confounded assessments of stem rust response. GH was crossed with a stem rust susceptible F 6 recombinant inbred line (RIL), GHB14 (M14), with similar maturity and an F 6:7 population was developed through single seed descent method. F 7 and F 8 RILs were tested along with the parents at different locations. The F 6 individual plants and both parents were genotyped using the 90K single nucleotide polymorphism (SNP) wheat array. Stem rust resistance QTL on the long arms of chromosomes 1B (QSrGH.cs-1BL) and 2A (QSrGH.cs-2AL) were detected. QSrGH.cs-1BL and QSrGH.cs-2AL were both contributed by GH and explained 22% and 18% adult plant stem rust response variation, respectively, among GH/M14 RIL population. RILs carrying combinations of these QTL reduced more than 14% stem rust severity compared to those that possessed QSrGH.cs-1BL and QSrGH.cs-2AL individually. QSrGH.cs1BL was demonstrated to be the same as Sr58/Lr46/Yr29/Pm39 through marker genotyping. Lines lacking QSrGH.cs-1BL were used to Mendelise QSrGH.cs-2AL. Based on genomic locations of previously catalogued stem rust resistance genes and the QSrGH.cs-2AL map, it appeared to represent a new APR locus and was permanently named Sr63. SNP markers associated with Sr63 were converted to kompetetive allele speci c PCR (KASP) assays and were validated on a set of durum cultivars. Key MessageAdult plant stem rust resistance locus, QSrGH.cs-2AL, was identi ed in durum wheat Glossy Huguenot and Mendelised as Sr63. Markers closely linked with Sr63 were developed.

Relocation of Sr48 to Chromosome 2D Using an Alternative Mapping Population and Development of a Closely Linked Marker Using Diverse Molecular Technologies

“…The identification and use of stem rust resistance genes has been an important strategy to effectively control this disease since the 1950s [7,25]. Sr48 is one of the race-specific resistance genes among over 63 formally designated resistance genes to date [26] https://shigen.nig.ac.jp/wheat/komugi/genes/symbolClassList.jsp, accessed on 20 September 2022) that is effective against Ug99 (H.S. Bariana, unpublished), and can, therefore, be useful when combined with other stem rust resistance genes.…”

Fighting wheat rusts in China: a look back and into the future

“…So far, 63 wheat stem rust resistance genes (Sr) have been identified worldwide (Mago et al 2022). In China, eight Sr genes, including Sr9e, Sr26,Sr31,Sr33,Sr37,Sr38,Sr47,and SrTt3, are still resistant to local Pgt races.…”

A Review on Major Rust Resistance Gene and Amino Acid Changes on Wheat (Triticum aestivum L)

“…Some of these genes are derived from monococcum wheat such as SrTm4 A m , Sr20, Sr21, Sr22b (Sr2+Fhb1 rec), Sr23, Sr35, Sr60, SrTm5, and some of these resistance genes are derived from durum wheat (Triticum turgidum) such as Sr2, Sr9e, Sr9g, Sr12, Sr14, Sr17, Sr63; similarly, some genes are derived from hexaploidy wheat of different cultivars such as Sr2, Sr5, Sr6, Sr7a, Sr7b, Sr8a, Sr8b, Sr9a, Sr9b, Sr9d, and some of resistance genes are derived from wild species such as Secale cereal, T. comosa, Aegilops speltoides, Triticum timopheevii ssp. Araraticum, Aegilops tauschii, inopyrum intermedium, rye cultivar, Aegilops searsii, Dasypyrum villosum, Aegilops geniculate such as Sr27, Sr34, Sr32, Sr33, Sr47, Sr53, and whole information is given in Table S1 [26][27][28][29][30][31][32][33][34][35][36]. be found almost anywhere wheat is farmed, and causes severe yield and economic losses [20].…”