Adult rat brain synaptic vesicles II. Lipid composition

Breckenridge, W. C.; Morgan, Ian G.; Jp, Zanetta; Vincendon, G.

doi:10.1016/0304-4165(73)90148-7

Cited by 180 publications

(85 citation statements)

References 28 publications

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“…terol-rich lipid microdomains (Breckenridge et al, 1973;Thiele et al, 2000;Chamberlain et al, 2001;Lang et al, 2001) that might mediate the synaptic localization of ␣-synuclein. Drugs that extract cholesterol from membranes (such as ␤-methylcyclodextrin) have been used to disrupt lipid rafts in primary neuronal culture, but also perturb synapse morphology (Hering et al, 2003).…”

Section: Resultsmentioning

confidence: 99%

Lipid Rafts Mediate the Synaptic Localization of α-Synuclein

Fortin¹,

Troyer²,

Nakamura³

et al. 2004

J. Neurosci.

493

458

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␣-Synuclein contributes to the pathogenesis of Parkinson's disease (PD), but its precise role in the disorder and its normal function remain poorly understood. Consistent with a presumed role in neurotransmitter release and its prominent deposition in the dystrophic neurites of PD, ␣-synuclein localizes almost exclusively to the nerve terminal. In brain extracts, however, ␣-synuclein behaves as a soluble, monomeric protein. Using a binding assay to characterize the association of ␣-synuclein with cell membranes, we find that ␣-synuclein binds saturably and with high affinity to characteristic intracellular structures that double label for components of lipid rafts. Biochemical analysis demonstrates the interaction of ␣-synuclein with detergent-resistant membranes and reveals a shift in electrophoretic mobility of the raft-associated protein. In addition, the A30P mutation associated with PD disrupts the interaction of ␣-synuclein with lipid rafts. Furthermore, we find that both the A30P mutation and raft disruption redistribute ␣-synuclein away from synapses, indicating an important role for raft association in the normal function of ␣-synuclein and its role in the pathogenesis of PD.

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Section: Resultsmentioning

confidence: 99%

Lipid Rafts Mediate the Synaptic Localization of α-Synuclein

Fortin¹,

Troyer²,

Nakamura³

et al. 2004

J. Neurosci.

493

458

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“…By the early 1970s, the very high concentration in brain synaptosomal plasma membranes (6) and synaptic vesicles (7) was described by Breckenridge and co-workers. Table 1 presents the DHA composition in the aminophospholipids of brain and other selected mammalian tissues (1,(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). It is apparent that the DHA content of the nervous system is very high.…”

Section: Dha Compositionmentioning

confidence: 99%

Mechanisms of action of docosahexaenoic acid in the nervous system

Salem

Litman

Kim

et al. 2001

Lipids

825

508

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This review describes (from both the animal and human literature) the biological consequences of losses in nervous system docosahexaenoate (DHA). It then concentrates on biological mechanisms that may serve to explain changes in brain and retinal function. Brief consideration is given to actions of DHA as a nonesterified fatty acid and as a docosanoid or other bioactive molecule. The role of DHA-phospholipids in regulating G-protein signaling is presented in the context of studies with rhodopsin. It is clear that the visual pigment responds to the degree of unsaturation of the membrane lipids. At the cell biological level, DHA is shown to have a protective role in a cell culture model of apoptosis in relation to its effects in increasing cellular phosphatidylserine (PS); also, the loss of DHA leads to a loss in PS. Thus, through its effects on PS, DHA may play an important role in the regulation of cell signaling and in cell proliferation. Finally, progress has been made recently in nuclear magnetic resonance studies to delineate differences in molecular structure and order in biomembranes due to subtle changes in the degree of phospholipid unsaturation.Paper no. L8776 in Lipids 36, 945-959 (September 2001)

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“…Phosphatidylcholine (PC) lipids account for more than half of phospholipids of most eukaryotic cellular and subcellular membranes [32][33][34]. Among them, POPC is the most abundant [35][36][37] and has been extensively used as a model compound for representation of natural PC mixtures [38]. While the MHF method has been used in synthesis of liposomes with other PC lipids [25][26][27]31], these studies have used molecules with two identical saturated fatty acid chains attached to the glycerol backbone, whereas lipid molecules of natural membranes usually contain two different fatty acids [39].…”

Section: Introductionmentioning

confidence: 99%

Microfluidic hydrodynamic focusing based synthesis of POPC liposomes for model biological systems

Mijajlovic

Wright

Živković

et al. 2013

Colloids and Surfaces B: Biointerfaces

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Elsevier's AAM Policy: Authors retain the right to use the accepted author manuscript for personal use, internal institutional use and for permitted scholarly posting provided that these are not for purposes of commercial use or systematic distribution.Elsevier believes that individual authors should be able to distribute their AAMs for their personal voluntary needs and interests, e.g. posting to their websites or their institution's repository, e-mailing to colleagues. However, our policies differ regarding the systematic aggregation or distribution of AAMs to ensure the sustainability of the journals to which AAMs are submitted. Therefore, deposit in, or posting to, subjectoriented or centralized repositories (such as PubMed Central), or institutional repositories with systematic posting mandates is permitted only under specific agreements between Elsevier and the repository, agency or institution, and only consistent with the publisher's policies concerning such repositories. AbstractLipid vesicles have received significant attention in areas ranging from pharmaceutical and biomedical engineering to novel materials and nanotechnology. Microfluidic-based synthesis of liposomes offers a number of advantages over the more traditional synthesis methods such as extrusion and sonication. One such microfluidic approach is microfluidic hydrodynamic focusing (MHF), which has been used to synthesize nanoparticles and vesicles of various lipids. We show here that this method can be utilized in synthesis of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) vesicles with controllable size. Since POPC is among the primary constituents of cellular membranes, this work is of direct applicability to modelling of biological systems and development of nanocontainers with higher biologic compatibility for pharmaceutical and medical applications.

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Adult rat brain synaptic vesicles II. Lipid composition

Cited by 180 publications

References 28 publications

Lipid Rafts Mediate the Synaptic Localization of α-Synuclein

Lipid Rafts Mediate the Synaptic Localization of α-Synuclein

Mechanisms of action of docosahexaenoic acid in the nervous system

Microfluidic hydrodynamic focusing based synthesis of POPC liposomes for model biological systems

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