The use of digital technology in education is currently important as for increased access to education through the perform various digital platforms, as well as by providing website as online learning media. The website can load text information, images, videos, animation, sound or a combination of these, and can be widely accessed. This research aims to develop an interactive website of Rawa Danau natural reserve, in Banten, to support conservation education through online learning. The 3D research and development (R & D) methodology approach was used which includes 3 stages; define, design, and develop. The define stage was the process of collecting data through the requirements analysis, curriculum analysis for biology conservation, and content analysis. The website of CARD biology conservation was developed in the design stage. In the develop stage, the website was assessed by the expert on both content material and learning media specialist. The results of the expert’s validations showed that the website was feasible to be used as online learning media for biology conservation education on both content material and learning media.