Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium

Tanksley, Steven D.; Grandillo, Silvana; Fulton, T. M.; Zamir, Dani; Eshed, Yuval; Pétiard, Vincent; López, J.; Beck-Bunn, T.

doi:10.1007/bf00223378

Cited by 358 publications

(135 citation statements)

References 12 publications

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“…Genetic resources promise to contribute favorable alleles to breeding programs, even if their performance with respect to the trait under consideration is worse than that of elite breeding material (Tanksley et al, 1996;Xiao et al, 1998;McCouch 2004;Falke et al, 2009b). NIL libraries provide the possibility of detecting such favorable QTL alleles as experimentally shown for plant height in rye (Falke et al, 2009b).…”

Section: Additional Quantitative Genetic Scenariosmentioning

confidence: 99%

Power and false-positive rate in QTL detection with near-isogenic line libraries

Falke

Frisch

2010

Heredity

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Libraries of near-isogenic lines (NILs) were used for quantitative trait locus (QTL) detection in model species and economically important crops. The experimental design and genetic architecture of the considered traits determine the statistical properties of QTL detection. The objectives of our simulation study were to (i) investigate the population sizes required to develop NIL libraries in barley and maize, (ii) compare NIL libraries with nonoverlapping and overlapping donor segments and (iii) study the number of QTLs and the size of their effects with respect to the power and the false-positive rate of QTL detection. In barley, the development of NIL libraries with target segment lengths of 10 cM and marker distances of 5 cM was possible using a BC 3 S 2 backcrossing scheme and population sizes of 140. In maize, population sizes larger than 200 were required. Selection for the recipient parent genome at markers flanking the target segments with distances between 5 and 10 cM was required for an efficient control of the false-positive rate. NIL libraries with nonoverlapping donor chromosome segments had a greater power of QTL detection and a smaller false-positive rate than libraries with overlapping segments. Major genes explaining 30% of the genotypic difference between the donor and recipient were successfully detected even with low heritabilities of 0.5, whereas for minor genes explaining 5 !or 10%, high heritabilities of 0.8 or 0.9 were required. The presented results can assist geneticists and breeders in the efficient development of NIL libraries for QTL detection.

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Section: Additional Quantitative Genetic Scenariosmentioning

confidence: 99%

Power and false-positive rate in QTL detection with near-isogenic line libraries

Falke

Frisch

2010

Heredity

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“…A previous study had examined the genetic control of domestication traits in the same population (Doganlar et al 2002b). The use of interspecific F 2 and advanced backcross populations has proven to be an effective and efficient way of mapping agronomic and horticultural traits in eggplant's relatives: tomato, potato and pepper (e.g., Bonierbale et al 1994;Tanksley et al 1996;Ben Chaim et al 2001). In addition to providing adequate phenotypic and DNA polymorphism which may be limiting in intraspecific eggplant populations (Nunome et al 2001), interspecific populations can supply the starting material for the introgression of valuable traits from wild species into eggplant cultivars.…”

Section: Introductionmentioning

confidence: 99%

QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species

et al. 2003

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An interspecific F 2 population from a cross between cultivated eggplant, Solanum melongena, and its wild relative, S. linnaeanum, was analyzed for quantitative trait loci (QTL) affecting leaf, flower, fruit and plant traits. A total of 58 plants were genotyped for 207 restriction fragment length polymorphism (RFLP) markers and phenotyped for 18 characters. One to eight loci were detected for each trait with a total of 63 QTL identified. Overall, 46% of the QTL had allelic effects that were the reverse of those predicted from the parental phenotypes. Wild alleles that were agronomically superior to the cultivated alleles were identified for 42% of the QTL identified for flowering time, flower and fruit number, fruit set, calyx size and fruit glossiness. Comparison of the map positions of eggplant loci with those for similar traits in tomato, potato and pepper revealed that 12 of the QTL have putative orthologs in at least one of these other species and that putative orthology was most often observed between eggplant and tomato. Traits showing potential orthology were: leaf length, shape and lobing; days to flowering; number of flowers per inflorescence; plant height and apex, leaf and stem hairiness. The functionally conserved loci included a major leaf lobing QTL (llob6.1) that is putatively orthologous to the potato leaf (c) and/or Petroselinum (Pts) mutants of tomato, two flowering time QTL (dtf1.1, dtf2.1) that also have putative counterparts in tomato and four QTL for trichomes that have potential orthologs in tomato and potato. These results support the mounting evidence of conservation of gene function during the evolution of eggplant and its relatives from their last common ancestor and indicate that this conservation was not limited to domestication traits.

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“…Recombination was lower at chromosome 2 and higher at chromosomes 1, 6, 7, 9, 11 and 12. Fragmentation of the introgressed segments after some selfing or backcross generations has been also demonstrated by Eshed et al (1992) and Tanksley et al (1996) respectively. Fulton et al (1997) also found the progressive decrease of the size of the introgressed fragments of S. peruvianum in S. lycopersicum.…”

Section: Discussionmentioning

confidence: 92%

Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato: exploitation of resistance to viruses

et al. 2013

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A set of introgression lines from S. peruvianum PI 126944 into the genetic background of cultivated tomato (S. lycopersicum) is being developed. Several generations were derived from three interspecific hybrids previously obtained. A lot of crosses and embryo rescue were required to obtain until the third backcross, due to the high incompatibility existing between tomato and PI 126944. Crosses between F1 plants allowed the obtaining of a pseudo-F2 generation. The same procedure was followed until pseudo-F6 generation. Additional crosses between plants of different generations were made in order to increase progeny. Among a total number of 263 molecular markers tested, 105 resulted polymorphic between tomato and PI 126944. This set of polymorphic markers consisted in 90 Simple Sequence Repeats and 15 Cleaved Amplified Polymorphic Sequences. Generations available were genotyped with these markers, observing a progressive reduction in the S. peruvianum genome in the most advanced. A reduction of incompatibility was achieved as a consequence of the S. peruvianum genome reduction. In addition, S. peruvianum genome was almost completely represented considering the different plants of the most advanced generations, so the set of ILs will be basically developed from them. Tomato yellow leaf curl virus (TYLCV) and Tomato spotted wilt virus (TSWV) resistance was evaluated in some generations, having been successfully introgressed and expressed into tomato background.

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Advanced backcross QTL analysis in a cross between an elite processing line of tomato and its wild relative L. pimpinellifolium

Cited by 358 publications

References 12 publications

Power and false-positive rate in QTL detection with near-isogenic line libraries

Power and false-positive rate in QTL detection with near-isogenic line libraries

QTL analysis of morphological traits in eggplant and implications for conservation of gene function during evolution of solanaceous species

Initial development of a set of introgression lines from Solanum peruvianum PI 126944 into tomato: exploitation of resistance to viruses

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