Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

Gao, Wendi; Zhao, Libo; Jiang, Zhuangde; Sun, Dong

doi:10.3390/app10207308

Cited by 9 publications

(3 citation statements)

References 170 publications

(194 reference statements)

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“…5k . However, optical observation suffers from imaging blurs and noises, which can lead to difficulty in distinguishing the sensing structure from backgrounds, especially for cases that are not at the focal plane 171 . To improve measurement accuracy, optical sensors usually use flexible materials or low-rigidity structures.…”

Section: Control Methodsmentioning

confidence: 99%

Advanced tools and methods for single-cell surgery

et al. 2022

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Highly precise micromanipulation tools that can manipulate and interrogate cell organelles and components must be developed to support the rapid development of new cell-based medical therapies, thereby facilitating in-depth understanding of cell dynamics, cell component functions, and disease mechanisms. This paper presents a literature review on micro/nanomanipulation tools and their control methods for single-cell surgery. Micromanipulation methods specifically based on laser, microneedle, and untethered micro/nanotools are presented in detail. The limitations of these techniques are also discussed. The biological significance and clinical applications of single-cell surgery are also addressed in this paper.

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Section: Control Methodsmentioning

confidence: 99%

Advanced tools and methods for single-cell surgery

et al. 2022

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“…Obtaining high-resolution, three-dimensional, information pertaining to intracellular architecture is a key task required for understanding complex biological mechanisms in a variety of research disciplines in cell biology and developmental biology 1 . In order to study and understand the diverse anatomical and functional complexities of living organisms it is important to be able to visualize how living cells dynamically organize their intracellular organelles while they undergo certain processes 2 . As such, single cell imaging of live cells, with high spatial and temporal resolution, is required.…”

Section: Acoustic Prison For Single Live Cell 3d Multi-imaging Enable...mentioning

confidence: 99%

Acoustic Prison for Single Live Cell 3D Multi-imaging Enabled by Light-sheet Microscopy

Richard

Vargas-Ordaz

Cadarso

et al. 2023

Preprint

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Having a system capable of obtaining high resolution, quantitative, subcellular information of indi- vidual live cells through imaging, while minimizing cell damage, is of great important in many fields of research. In recent years, optofluidic light-sheet fluorescent microscopy (LSFM) has emerged as a powerful tool providing a low photo-toxic imaging method utilising the fluidic environment offered by microfluidics. Here, the benefits of LSFM were integrated with an acoustic single cell prison for precise single cell handling and 3D multi-imaging in a semi-automated manner. The compact, monolithic, acousto-optofluidic platform employed standing surface acoustic waves (SSAWs) to sequentially trap an individual cell on either side of an imaging region, which gathered planar, cross-sectional images of the cell. Through post-image processing, 3D volumetric images of the cell were reconstructed and the results between the first and second rounds of imaging were directly comparable. Furthermore, the acoustic prison advantageously positions the cells in the upper region of the channel, enabling the ability to accurately compare temporal changes in cell morphology; a capability that can lead to advancements in therapeutics and drug delivery to access responses of cells to stimuli over time.

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“…To increase automation extent and reduce operation damage in cell surgery, force and inclination sensing provide robotic manipulation tasks with a real-time feedback via interaction force regulation [5], [6] and operation orientation control [7], [8]. However, biological signals do not only suffer from small amplitude but they are also accompanied by strong background noise from the cellular environment [9], [10]; therefore, force sensing techniques with high sensitivity and resolution are in urgent demand.…”

Section: Introductionmentioning

confidence: 99%

A high resolution capacitive MEMS force sensor with novel bionic swallow comb arrays for multiphysics measurement

Gao¹

2022

Preprint

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Precise force sensing is essential for the mechanical characterization and robotic micromanipulation of biological<br>targets. In this work, a high-resolution MEMS capacitive force sensor was proposed for measuring ultralow multiphysics. A bionic swallow structure that contained multiple feathered comb arrays was designed for reducing chip dimension and eliminating undesirable mechanical cross-coupling effect. The comb structure was optimized for maximum sensitivity, linearity, and compact chip size. Utilizing a novel interconnection configuration, interferences derived from parasitic capacitance and electrostatic forces exerted negligible effects on the sensor output. The proposed bionic force sensor was fabricated following a simple three-mask process and integrated with ASIC readouts. Its measuring sensitivity was 7.151 fF/nm, 0.529 aF/nN, and 4.247 pF/g for displacement, force, and inclination measurements, respectively. The proposed sensor had a large measurement range of 1000.00 nm and 13.83 µN with a high linearity of 0.9998. The 1-σ resolution was 0.0328 nm and 0.4436 nN, and the noise floor resolution was 0.0044 nm √? and 0.0597 nN/ √? for<br>displacement and force measurements, respectively. The bias stability of Allan deviance was 0.0050 nm and 0.0678 nN at an integration time of 0.65 s. The proposed bionic swallow sensor exhibited considerable improvement over existing capacitive sensors and feasibility for ultralow multiphysics measurement in biomedical applications. <br>

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Advanced Biological Imaging for Intracellular Micromanipulation: Methods and Applications

Cited by 9 publications

References 170 publications

Advanced tools and methods for single-cell surgery

Advanced tools and methods for single-cell surgery

Acoustic Prison for Single Live Cell 3D Multi-imaging Enabled by Light-sheet Microscopy

A high resolution capacitive MEMS force sensor with novel bionic swallow comb arrays for multiphysics measurement

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